Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,916,944 A→G K229K (AAA→AAG yebS → inner membrane PqiA domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,916,9440AG100.0% 170.3 / NA 48K229K (AAA→AAGyebSinner membrane PqiA domain protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG  >  NC_000913/1916820‑1917062
                                                                                                                            |                                                                                                                      
gtgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTgtctgtct                                                                                                          >  1:446412/1‑139 (MQ=255)
 tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt                                                                                                         >  1:64818/1‑139 (MQ=255)
 tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGAAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt                                                                                                         >  1:827758/1‑139 (MQ=255)
             ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             <  2:827758/139‑1 (MQ=255)
             ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             <  1:137638/139‑1 (MQ=255)
                ttttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc                                                                                          <  1:302217/139‑1 (MQ=255)
                ttttGTGGCGTTGGTGATTCTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc                                                                                          <  2:630710/139‑1 (MQ=255)
                  ttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        <  1:960293/139‑1 (MQ=255)
                            ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct                                                                                                              <  2:259404/107‑1 (MQ=255)
                            ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct                                                                                                              >  1:259404/1‑107 (MQ=255)
                                 ttttAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCg                                                                         <  2:446412/139‑1 (MQ=255)
                                       cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             >  1:221540/1‑113 (MQ=255)
                                       cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             <  2:221540/113‑1 (MQ=255)
                                          cgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTg                                                                >  1:743969/1‑139 (MQ=255)
                                            gTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc                                                              >  1:729003/1‑139 (MQ=255)
                                              gACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg                                                            <  1:823460/139‑1 (MQ=255)
                                                cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg                                                          <  2:1212328/139‑1 (MQ=255)
                                                cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg                                                          <  1:137132/139‑1 (MQ=255)
                                                   tGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTg                                                       <  2:15283/139‑1 (MQ=255)
                                                         aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt                                                                     <  1:566084/119‑1 (MQ=255)
                                                         aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt                                                                     >  2:566084/1‑119 (MQ=255)
                                                                   cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg                                                                                                                 >  2:137370/1‑65 (MQ=255)
                                                                   cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg                                                                                                                 <  1:137370/65‑1 (MQ=255)
                                                                   cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt                                                                                    <  2:124798/94‑1 (MQ=255)
                                                                   cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt                                                                                    >  1:124798/1‑94 (MQ=255)
                                                                     aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt                                     >  1:946743/1‑139 (MQ=255)
                                                                     aagaaCTGTGGGAGAGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt                                     >  1:751585/1‑139 (MQ=255)
                                                                                   cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa                       <  2:780138/139‑1 (MQ=255)
                                                                                     aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        <  1:257257/72‑1 (MQ=255)
                                                                                     aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        >  2:257257/1‑72 (MQ=255)
                                                                                       tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        >  2:1217791/1‑70 (MQ=255)
                                                                                       tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        <  1:1217791/70‑1 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             >  2:973485/1‑56 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt                                                                                             <  1:973485/56‑1 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt                                 >  2:253143/1‑116 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt                                 <  1:253143/116‑1 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg          >  1:442645/1‑139 (MQ=255)
                                                                                                aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCACTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg          >  2:826193/1‑139 (MQ=255)
                                                                                                  cgcCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCt        >  2:946447/1‑139 (MQ=255)
                                                                                                    ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa                       >  1:1316850/1‑122 (MQ=255)
                                                                                                    ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa                       <  2:1316850/122‑1 (MQ=255)
                                                                                                     cccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTggg     >  2:679817/1‑139 (MQ=255)
                                                                                                        gCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGggcgg  <  2:743969/139‑1 (MQ=255)
                                                                                                                 aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg                                                            >  2:580156/1‑72 (MQ=255)
                                                                                                                 aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg                                                            <  1:580156/72‑1 (MQ=255)
                                                                                                                      cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        <  2:624540/39‑1 (MQ=255)
                                                                                                                      cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg                                                                                        >  1:624540/1‑39 (MQ=255)
                                                                                                                         gaAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg                                                            >  1:1284003/1‑64 (MQ=255)
                                                                                                                            |                                                                                                                      
GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG  >  NC_000913/1916820‑1917062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: