Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,916,944 | A→G | K229K (AAA→AAG) | yebS → | inner membrane PqiA domain protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,916,944 | 0 | A | G | 100.0% | 170.3 / NA | 48 | K229K (AAA→AAG) | yebS | inner membrane PqiA domain protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24) |
GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG > NC_000913/1916820‑1917062 | gtgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTgtctgtct > 1:446412/1‑139 (MQ=255) tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt > 1:64818/1‑139 (MQ=255) tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGAAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt > 1:827758/1‑139 (MQ=255) ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 2:827758/139‑1 (MQ=255) ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 1:137638/139‑1 (MQ=255) ttttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc < 1:302217/139‑1 (MQ=255) ttttGTGGCGTTGGTGATTCTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc < 2:630710/139‑1 (MQ=255) ttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:960293/139‑1 (MQ=255) ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct < 2:259404/107‑1 (MQ=255) ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct > 1:259404/1‑107 (MQ=255) ttttAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCg < 2:446412/139‑1 (MQ=255) cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt > 1:221540/1‑113 (MQ=255) cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 2:221540/113‑1 (MQ=255) cgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTg > 1:743969/1‑139 (MQ=255) gTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc > 1:729003/1‑139 (MQ=255) gACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg < 1:823460/139‑1 (MQ=255) cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg < 2:1212328/139‑1 (MQ=255) cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg < 1:137132/139‑1 (MQ=255) tGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTg < 2:15283/139‑1 (MQ=255) aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:566084/119‑1 (MQ=255) aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt > 2:566084/1‑119 (MQ=255) cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg > 2:137370/1‑65 (MQ=255) cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg < 1:137370/65‑1 (MQ=255) cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt < 2:124798/94‑1 (MQ=255) cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt > 1:124798/1‑94 (MQ=255) aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt > 1:946743/1‑139 (MQ=255) aagaaCTGTGGGAGAGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt > 1:751585/1‑139 (MQ=255) cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa < 2:780138/139‑1 (MQ=255) aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:257257/72‑1 (MQ=255) aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 2:257257/1‑72 (MQ=255) tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 2:1217791/1‑70 (MQ=255) tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:1217791/70‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt > 2:973485/1‑56 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 1:973485/56‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt > 2:253143/1‑116 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt < 1:253143/116‑1 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg > 1:442645/1‑139 (MQ=255) aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCACTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg > 2:826193/1‑139 (MQ=255) cgcCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCt > 2:946447/1‑139 (MQ=255) ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa > 1:1316850/1‑122 (MQ=255) ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa < 2:1316850/122‑1 (MQ=255) cccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTggg > 2:679817/1‑139 (MQ=255) gCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGggcgg < 2:743969/139‑1 (MQ=255) aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg > 2:580156/1‑72 (MQ=255) aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg < 1:580156/72‑1 (MQ=255) cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 2:624540/39‑1 (MQ=255) cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 1:624540/1‑39 (MQ=255) gaAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg > 1:1284003/1‑64 (MQ=255) | GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG > NC_000913/1916820‑1917062 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |