Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,782	G→A	*221* (TAG→TAA)  D686D (GAC→GAT)	exoX → ptrB ←	exodeoxyribonuclease 10, DNA exonuclease X protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,782	0	G	A	100.0%	167.1 / NA	52	*221* (TAG→TAA)  D686D (GAC→GAT)	exoX ptrB	exodeoxyribonuclease 10, DNA exonuclease X protease II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/28);  total (24/28)

TTGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATA  >  NC_000913/1926646‑1926915                                                                                                                                         |                                                                                                                                      ttGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATc                                                                                                                                     <  2:281212/139‑1 (MQ=255) ttGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATc                                                                                                                                     <  2:313251/139‑1 (MQ=255) ttGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATc                                                                                                                                     >  1:716358/1‑139 (MQ=255)                                                  gggCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCa                                                                                    <  1:1063560/139‑1 (MQ=255)                                                          tGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCAt                                                                            <  2:954683/139‑1 (MQ=255)                                                                ggTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTAc                                                                      >  2:967291/1‑139 (MQ=255)                                                                  ttATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGc                                                                    >  2:1000657/1‑139 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTa                                                                                                                           <  1:1307522/75‑1 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTa                                                                                                                           >  2:1307522/1‑75 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                         <  2:209725/77‑1 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGc                                                                                                                         >  1:209725/1‑77 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTgcg                                                                                                        <  1:320400/94‑1 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTgcg                                                                                                        >  2:320400/1‑94 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAc                                                                                              <  1:1317864/104‑1 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAc                                                                                              >  2:1317864/1‑104 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              >  1:580195/1‑120 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              <  2:580195/120‑1 (MQ=255)                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACg                                                           >  1:75258/1‑139 (MQ=255)                                                                               tGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTcc                                                                                                             <  2:1354854/85‑1 (MQ=255)                                                                               tGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGAttt                                                       >  2:1305511/1‑139 (MQ=255)                                                                               tGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAAAATTcc                                                                                                             >  1:1354854/1‑85 (MQ=255)                                                                                 gACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGTGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTaa                                                     <  1:967291/139‑1 (MQ=255)                                                                                  aCAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTaaa                                                    <  1:1305511/139‑1 (MQ=255)                                                                                  aCAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTaaa                                                    <  1:745365/139‑1 (MQ=255)                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              >  2:418361/1‑109 (MQ=255)                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              <  1:418361/109‑1 (MQ=255)                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGa                                                >  2:1040731/1‑139 (MQ=255)                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGAATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGa                                                >  2:371461/1‑139 (MQ=255)                                                                                      cATGAGCCCGGAGCTGCGTATAACACTGAGACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGATCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGa                                                >  2:913718/1‑139 (MQ=255)                                                                                       aTGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAc                                               <  2:716358/139‑1 (MQ=255)                                                                                        tGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGa                                                                                          >  1:383750/1‑95 (MQ=255)                                                                                        tGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGa                                                                                          <  2:383750/95‑1 (MQ=255)                                                                                                gAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTAc                                      >  2:768560/1‑139 (MQ=255)                                                                                                gAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTAc                                      >  1:80797/1‑139 (MQ=255)                                                                                                          tAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGAATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGcc                            <  1:371461/139‑1 (MQ=255)                                                                                                           aaCACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                              <  1:977979/104‑1 (MQ=255)                                                                                                           aaCACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                              >  2:977979/1‑104 (MQ=255)                                                                                                                tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGa                                                                                          >  2:427549/1‑71 (MQ=255)                                                                                                                tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGa                                                                                          <  1:427549/71‑1 (MQ=255)                                                                                                                tGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                      <  1:768560/139‑1 (MQ=255)                                                                                                                  aaaCATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                    <  1:1069337/139‑1 (MQ=255)                                                                                                                     cATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt                 <  1:424546/139‑1 (MQ=255)                                                                                                                        tATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              >  2:300163/1‑75 (MQ=255)                                                                                                                        tATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                              <  1:300163/75‑1 (MQ=255)                                                                                                                           cTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc           <  2:1201707/139‑1 (MQ=255)                                                                                                                             ggAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                      >  2:495046/1‑126 (MQ=255)                                                                                                                             ggAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                      <  1:495046/126‑1 (MQ=255)                                                                                                                              gAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGCCCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga        <  1:913718/139‑1 (MQ=255)                                                                                                                                  aTACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCaa    <  1:511163/139‑1 (MQ=255)                                                                                                                                    aCTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGAtt                                         <  2:168597/100‑1 (MQ=255)                                                                                                                                    aCTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGAtt                                         >  1:168597/1‑100 (MQ=255)                                                                                                                                    aCTTAATCCGCAGGCGAAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTACAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATa  >  1:368657/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      TTGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATA  >  NC_000913/1926646‑1926915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑