Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,874 T→C T481A (ACC→GCC)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,8740TC100.0% 153.6 / NA 44T481A (ACC→GCC) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGC  >  NC_000913/1934738‑1934999
                                                                                                                                        |                                                                                                                             
cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCaa                                                                                                                             >  2:43387/1‑139 (MQ=255)
         aTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCa                                                                                                                    <  2:884130/139‑1 (MQ=255)
                  aGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCaa                                                                                                           >  2:1001423/1‑139 (MQ=255)
                     ccTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACg                                                                                                         <  2:1231600/138‑1 (MQ=255)
                     ccTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACg                                                                                                         >  1:1231600/1‑138 (MQ=255)
                               tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGt                                                                                              >  1:889175/1‑139 (MQ=255)
                                   aaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                          <  1:43387/139‑1 (MQ=255)
                                       gTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGc                                                                                                             <  1:1021352/116‑1 (MQ=255)
                                       gTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGc                                                                                                             >  2:1021352/1‑116 (MQ=255)
                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                                                                         <  1:130779/139‑1 (MQ=255)
                                                                   ccGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                          >  1:1099545/1‑107 (MQ=255)
                                                                   ccGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                          <  2:1099545/107‑1 (MQ=255)
                                                                       tAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                      >  2:1184211/1‑139 (MQ=255)
                                                                          aaTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc                                                   >  2:464610/1‑139 (MQ=255)
                                                                                    aaGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTaa                                         <  2:889175/139‑1 (MQ=255)
                                                                                         tAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa                                                    >  2:1168084/1‑123 (MQ=255)
                                                                                         tAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa                                                    <  1:1168084/123‑1 (MQ=255)
                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                                                                         >  2:752248/1‑101 (MQ=255)
                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                                                                         <  1:752248/101‑1 (MQ=255)
                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       >  2:434081/1‑103 (MQ=255)
                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       <  1:434081/103‑1 (MQ=255)
                                                                                              gcAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTAc                               <  1:1184211/139‑1 (MQ=255)
                                                                                                     ttACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTc                        <  1:1001423/139‑1 (MQ=255)
                                                                                                       aCTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                      >  2:497504/1‑139 (MQ=255)
                                                                                                            aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       >  2:15076/1‑85 (MQ=255)
                                                                                                            aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       <  1:15076/85‑1 (MQ=255)
                                                                                                            aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGAGCATCCGTGTCCATCGCCCCCGCCTCAGTAATGGAGCTTACCCATTTCCAGGctt                 >  1:1335852/1‑139 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                  >  1:217090/1‑57 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                  <  2:217090/57‑1 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       >  2:763814/1‑84 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       <  1:763814/84‑1 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCt                                               >  1:125656/1‑108 (MQ=255)
                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCt                                               <  2:125656/108‑1 (MQ=255)
                                                                                                                  ttCCAGGAACGACCATCACGGGCAATCATCGCCAcc                                                                                                                  <  1:93992/36‑1 (MQ=38)
                                                                                                                  ttCCAGGAACGACCATCACGGGCAATCATCGCCAcc                                                                                                                  >  2:93992/1‑36 (MQ=255)
                                                                                                                         aaCGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                      <  1:16440/89‑1 (MQ=255)
                                                                                                                         aaCGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                      >  2:16440/1‑89 (MQ=255)
                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                                        >  2:1167437/1‑101 (MQ=255)
                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                                        <  1:1167437/101‑1 (MQ=255)
                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc  <  1:497504/139‑1 (MQ=255)
                                                                                                                            gACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       >  2:1320722/1‑69 (MQ=255)
                                                                                                                            gACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                       <  1:1320722/69‑1 (MQ=255)
                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGcc                                                                                       >  1:1131646/1‑52 (MQ=255)
                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGcc                                                                                       <  2:1131646/52‑1 (MQ=255)
                                                                                                                                        |                                                                                                                             
CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGC  >  NC_000913/1934738‑1934999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑