Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,993	G→A	G111E (GGG→GAG)	znuC →	zinc ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,993	0	G	A	100.0%	125.0 / NA	39	G111E (GGG→GAG)	znuC	zinc ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/18);  total (21/18)

ACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGC  >  NC_000913/1942859‑1943111                                                                                                                                       |                                                                                                                       aCTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCAc                                                                                                                    <  2:908012/139‑1 (MQ=255)          cGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAAc                                                                                                           >  1:1221386/1‑139 (MQ=255)              tATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCAc                                                                                                       <  2:1221386/139‑1 (MQ=255)                                          ccacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAAc                                                                           >  1:209135/1‑139 (MQ=255)                                             cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        >  1:404911/1‑139 (MQ=255)                                                       gACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCAc                                                                                                                    >  2:938469/1‑85 (MQ=255)                                                       gACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCAc                                                                                                                    <  1:938469/85‑1 (MQ=255)                                                        aCCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCtggtg                                                                                  <  1:1230548/118‑1 (MQ=255)                                                        aCCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCtggtg                                                                                  >  2:1230548/1‑118 (MQ=255)                                                                    tttttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        >  1:1321721/1‑116 (MQ=255)                                                                    tttttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        <  2:1321721/116‑1 (MQ=255)                                                                      tttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGTAAAAg                                                                                            >  2:867499/1‑94 (MQ=255)                                                                      tttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                            <  1:867499/94‑1 (MQ=255)                                                                              tacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCg                                       >  1:1166481/1‑139 (MQ=255)                                                                               acgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                      <  2:209135/139‑1 (MQ=255)                                                                                               aaaGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGc                      >  2:1278742/1‑139 (MQ=255)                                                                                                       tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        >  1:985712/1‑81 (MQ=255)                                                                                                       tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        <  2:985712/81‑1 (MQ=255)                                                                                                       tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGc                                                   >  1:373712/1‑102 (MQ=255)                                                                                                       tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGc                                                   <  2:373712/102‑1 (MQ=255)                                                                                                       tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGGTGaa              >  2:902080/1‑139 (MQ=255)                                                                                                       tatGTTGCCTGCAATGAAACGTGGCCAGGCCGAGCACCTGATTAACGCACCGATACAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGTGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGaa              <  1:902080/139‑1 (MQ=255)                                                                                                           ttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                    >  2:1221484/1‑97 (MQ=255)                                                                                                           ttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                    <  1:1221484/97‑1 (MQ=255)                                                                                                            tGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTa                                                                                                             >  1:993386/1‑39 (MQ=255)                                                                                                            tGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTa                                                                                                             <  2:993386/39‑1 (MQ=255)                                                                                                             gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACt        >  2:120514/1‑139 (MQ=255)                                                                                                              ccTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTc       <  2:1166481/139‑1 (MQ=255)                                                                                                                  cACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAgg   >  2:933301/1‑139 (MQ=255)                                                                                                                  cACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACACAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAgg   >  1:1155903/1‑139 (MQ=255)                                                                                                                   aCTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGc  <  1:1138010/139‑1 (MQ=255)                                                                                                                       aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCtggtg                                                                                  <  2:241326/55‑1 (MQ=255)                                                                                                                       aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCtggtg                                                                                  >  1:241326/1‑55 (MQ=255)                                                                                                                            tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                         >  2:1347415/1‑59 (MQ=255)                                                                                                                            tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                         <  1:1347415/59‑1 (MQ=255)                                                                                                                            tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                       >  2:891748/1‑61 (MQ=255)                                                                                                                            tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                       <  1:891748/61‑1 (MQ=255)                                                                                                                                   gCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        <  2:470689/53‑1 (MQ=255)                                                                                                                                   gCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                        >  1:470689/1‑53 (MQ=255)                                                                                                                                       |                                                                                                                       ACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGC  >  NC_000913/1942859‑1943111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑