Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 185,465 G→A G161G (GGC→GGT dapD ← 2,3,4,5‑tetrahydropyridine‑2‑carboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913185,4650GA100.0% 147.1 / NA 46G161G (GGC→GGTdapD2,3,4,5‑tetrahydropyridine‑2‑carboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

CATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACGCCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCAC  >  NC_000913/185336‑185584
                                                                                                                                 |                                                                                                                       
cATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGaa                                                                                                                >  2:732712/1‑139 (MQ=255)
cATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGaa                                                                                                                >  1:1278910/1‑139 (MQ=255)
  tGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAg                                                                                                              >  2:537385/1‑139 (MQ=255)
                    cttcGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCAGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAAt                                                                                            <  1:548133/139‑1 (MQ=255)
                                           aCTTCAGAGCGCGCGCCGATGAAGTAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCg                                                                     >  1:928412/1‑139 (MQ=255)
                                           aCTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCg                                                                     >  1:887361/1‑139 (MQ=255)
                                                 gagCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGa                                                                                                         >  1:1167696/1‑97 (MQ=255)
                                                 gagCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGa                                                                                                         <  2:1167696/97‑1 (MQ=255)
                                                      cgcgcCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCa                                                          >  2:1197419/1‑139 (MQ=255)
                                                             aTGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATgg                                                   <  2:391509/139‑1 (MQ=255)
                                                              tGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATc                                                           >  2:1356319/1‑130 (MQ=255)
                                                              tGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATc                                                           <  1:1356319/130‑1 (MQ=255)
                                                              tGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGt                                                  >  1:597745/1‑139 (MQ=255)
                                                               gAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTg                                                 <  2:480659/139‑1 (MQ=255)
                                                               gAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTg                                                 <  2:1278910/139‑1 (MQ=255)
                                                                 aGCAATTATCTTCAATCATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCTCAGGTATCAACCATGGCGcc                                               <  2:928412/139‑1 (MQ=255)
                                                                 aGCAATTATCTCCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGcc                                               <  2:887361/139‑1 (MQ=255)
                                                                     aTTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGcc                                               >  2:398825/1‑135 (MQ=255)
                                                                     aTTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGcc                                               <  1:398825/135‑1 (MQ=255)
                                                                           ttCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTg                                                 <  1:1325121/127‑1 (MQ=255)
                                                                           ttCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTg                                                 >  2:1325121/1‑127 (MQ=255)
                                                                           ttCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACa                                     >  2:824713/1‑139 (MQ=255)
                                                                            tCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACat                                    >  2:624228/1‑139 (MQ=255)
                                                                             cAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                          >  1:633561/1‑68 (MQ=255)
                                                                             cAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTgg                                                                                                          <  2:633561/68‑1 (MQ=255)
                                                                             cAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACata                                   >  1:998617/1‑139 (MQ=255)
                                                                                tgatgGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATgc                                >  2:112843/1‑139 (MQ=255)
                                                                                   tgGTTGGGTTAGCCTGCAGCGGTTCCAGTACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGccc                                                                  >  2:1334973/1‑102 (MQ=255)
                                                                                   tgGTTGGGTTAGCCTGCAGCGGTTCCAGTACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGccc                                                                  <  1:1334973/102‑1 (MQ=255)
                                                                                            tAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACaa                                                                                  <  1:446911/77‑1 (MQ=255)
                                                                                            tAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACaa                                                                                  >  2:446911/1‑77 (MQ=255)
                                                                                              gCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTAcc                                                                                               >  2:1231340/1‑62 (MQ=255)
                                                                                              gCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTAcc                                                                                               <  1:1231340/62‑1 (MQ=255)
                                                                                              gCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGt                  <  2:597745/139‑1 (MQ=255)
                                                                                               ccTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTg                                                                                         <  1:513654/67‑1 (MQ=255)
                                                                                               ccTGCAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTg                                                                                         >  2:513654/1‑67 (MQ=255)
                                                                                                   cAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAgg                                                               <  2:42850/89‑1 (MQ=255)
                                                                                                   cAGCGGTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAgg                                                               >  1:42850/1‑89 (MQ=255)
                                                                                                       ggTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCa         <  1:112843/139‑1 (MQ=255)
                                                                                                        gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATgcgc                              <  2:1344733/117‑1 (MQ=255)
                                                                                                        gTTCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATgcgc                              >  1:1344733/1‑117 (MQ=255)
                                                                                                         ttCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTgcg                                                                                       >  1:1145044/1‑59 (MQ=255)
                                                                                                         ttCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTgcg                                                                                       <  2:1145044/59‑1 (MQ=255)
                                                                                                         ttCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCAt        <  1:1314923/138‑1 (MQ=255)
                                                                                                         ttCCAGCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCAt        >  2:1314923/1‑138 (MQ=255)
                                                                                                              gCACGCCGCCGATGCCCACACCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCAc  >  2:1304001/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                       
CATGGAAATGACGGAACCTTCTTCGACAATCACCCCTTCAACCACTTCAGAGCGCGCGCCGATGAAGCAATTATCTTCAATGATGGTTGGGTTAGCCTGCAGCGGTTCCAGCACGCCGCCGATGCCCACGCCACCGGAAAGGTGGACGTTTTTACCAATCTGCGCACAAGAACCGACGGTCGCCCAGGTATCAACCATGGTGCCTTCATCAACATATGCGCCGATGTTGACGTAAGACGGCATCAGCAC  >  NC_000913/185336‑185584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: