Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,085,831 A→G L411P (CTG→CCG)  plaP ← putrescine importer, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,8310AG100.0% 706.7 / NA 194L411P (CTG→CCG) plaPputrescine importer, low affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (98/96);  total (98/96)

Only 100 of 194 total aligned reads displayed.

AGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085698‑2085964
                                                                                                                                      |                                                                                                                                     
        aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCACCGGt                                                                                                                          <  1:1005555/139‑1 (MQ=255)
        aTGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  2:441459/139‑1 (MQ=255)
               gTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGGTCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     >  1:978747/1‑137 (MQ=255)
               gTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGca                                                                                                                   >  2:607242/1‑139 (MQ=255)
                   tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGcaccac                                                                                                               <  2:979528/139‑1 (MQ=255)
                    cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcaca                                                                                                              >  2:119792/1‑139 (MQ=255)
                      tACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:7085/139‑1 (MQ=255)
                           cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  2:1228934/1‑139 (MQ=255)
                           cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  2:1335185/139‑1 (MQ=255)
                            ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      <  2:610013/139‑1 (MQ=255)
                            ggAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      <  2:1076524/139‑1 (MQ=255)
                              aaCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAGTCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                    <  2:915599/139‑1 (MQ=255)
                              aaCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                    <  1:296738/139‑1 (MQ=255)
                               aCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                   <  2:1034296/139‑1 (MQ=255)
                                ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGgcg                                                                                                                                    >  2:156318/1‑105 (MQ=255)
                                ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGgcg                                                                                                                                    <  1:156318/105‑1 (MQ=255)
                                ccGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     <  1:1152235/120‑1 (MQ=255)
                                 cGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAAc                                                                                                 <  1:235823/139‑1 (MQ=255)
                                     ttACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                       <  2:1157173/113‑1 (MQ=255)
                                     ttACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAg                                                                                                                       >  1:1157173/1‑113 (MQ=255)
                                     ttACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATc                                                                                                          <  2:1264478/126‑1 (MQ=255)
                                        cGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACt                                                                                          >  1:33269/1‑139 (MQ=255)
                                             gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            >  2:413115/1‑116 (MQ=255)
                                             gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:413115/116‑1 (MQ=255)
                                             gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       <  1:1004194/121‑1 (MQ=255)
                                             gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAg                                                                                     >  1:1209025/1‑139 (MQ=255)
                                             gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCAACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  2:1004194/1‑121 (MQ=255)
                                              ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAga                                                                                    >  2:954853/1‑138 (MQ=255)
                                              ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                    >  2:175971/1‑139 (MQ=255)
                                              ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTAAGCGCACCACACATCGGCAGGAACAGATAcctgtctc                                                                                    >  1:871632/1‑132 (MQ=255)
                                                ctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGgcg                                                                                                                                    <  1:1244766/89‑1 (MQ=255)
                                                  tGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATc                                                                                >  2:1271047/1‑139 (MQ=255)
                                                   ggTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGGTTAACCGACGGCGCACCAACGGTCAGCGCACCACACATCGGCAgg                                                                                                    <  1:610122/119‑1 (MQ=255)
                                                       aCGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAgaaagctcaat                                                                           <  1:954853/139‑11 (MQ=255)
                                                          cAAGCCAGGTAAATCAGATCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  1:1109215/139‑1 (MQ=255)
                                                             gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCt                                                                     <  2:33269/139‑1 (MQ=255)
                                                                       tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCtt                                                           >  2:1299159/1‑139 (MQ=255)
                                                                           aCCGATAGCAGCCCTGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  >  1:199043/1‑96 (MQ=255)
                                                                           aCCGATAGCAGCCCTGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:199043/96‑1 (MQ=255)
                                                                            ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGTTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      <  2:976757/139‑1 (MQ=255)
                                                                            ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                <  2:86805/97‑1 (MQ=255)
                                                                            ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        >  2:62033/1‑121 (MQ=255)
                                                                               aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATc                                                                                                          >  1:914730/1‑84 (MQ=255)
                                                                                tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:544825/91‑1 (MQ=255)
                                                                                tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  >  1:544825/1‑91 (MQ=255)
                                                                                tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  2:560863/1‑135 (MQ=255)
                                                                                   cagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                               <  2:57724/139‑1 (MQ=255)
                                                                                   cagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                               <  1:1268294/139‑1 (MQ=255)
                                                                                   cagcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                               <  1:672824/139‑1 (MQ=255)
                                                                                     gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     <  1:597260/67‑1 (MQ=255)
                                                                                          gATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                       >  2:1242504/1‑76 (MQ=255)
                                                                                           aTCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                       >  1:1300672/1‑139 (MQ=255)
                                                                                            tCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCga                                      <  2:632620/139‑1 (MQ=255)
                                                                                             cAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAgcg                                                                                                                     <  1:561224/59‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAAc                                                                                                                             <  1:25009/48‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:89606/1‑51 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGt                                                                                                                          >  1:1029715/1‑51 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:683902/65‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  2:1152719/65‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      <  2:508866/71‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                      >  1:508866/1‑71 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                        <  1:615421/101‑1 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                                  >  2:114090/1‑139 (MQ=255)
                                                                                                aCCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATg                                  >  2:1223038/1‑139 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGgcg                                                                                                                                    <  2:1024795/36‑1 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  2:1178570/46‑1 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAg                                                                                               >  2:706841/1‑73 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATa                                                                                            <  2:1098376/76‑1 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATa                                                                                            >  1:1098376/1‑76 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGa                                                                                       >  1:458831/1‑81 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             >  1:321740/1‑139 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             >  2:480296/1‑139 (MQ=255)
                                                                                                     gAACCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCACACCAACGGTCAGCGCACCACACATCgg                                                                                                        <  1:123169/64‑1 (MQ=255)
                                                                                                       aCCATTGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                  <  2:563169/68‑1 (MQ=255)
                                                                                                           ttGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              <  1:65761/100‑1 (MQ=255)
                                                                                                           ttGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACg                                                              >  2:65761/1‑100 (MQ=255)
                                                                                                           ttGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                      >  2:672984/1‑108 (MQ=255)
                                                                                                            tGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCa                                                                                                                        <  1:887672/41‑1 (MQ=255)
                                                                                                            tGAGCTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCa                                                                                                                        >  2:887672/1‑41 (MQ=255)
                                                                                                                cTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGt                                                                                                                          <  1:461762/35‑1 (MQ=255)
                                                                                                                cTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTGTTGTTACGCTTCTCAc                                                      >  2:904922/1‑103 (MQ=255)
                                                                                                                cTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGa                  >  2:414925/1‑139 (MQ=255)
                                                                                                                cTTTCTTCCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGa                  >  1:12873/1‑139 (MQ=255)
                                                                                                                  ttcttcCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        >  2:335862/1‑51 (MQ=255)
                                                                                                                  ttcttcCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        <  1:335862/51‑1 (MQ=255)
                                                                                                                   tcttcCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACcacaca                                                                                                            <  1:630044/46‑1 (MQ=255)
                                                                                                                     ttcCA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                   >  1:842298/1‑69 (MQ=255)
                                                                                                                       ccA‑GGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGaa           <  1:414925/139‑1 (MQ=255)
                                                                                                                           ggTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                           >  2:46087/1‑56 (MQ=255)
                                                                                                                              tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             <  2:58696/115‑1 (MQ=255)
                                                                                                                              tAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                             >  1:58696/1‑115 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                   >  2:614013/1‑59 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                   <  1:614013/59‑1 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                           <  1:768434/67‑1 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                >  2:722813/1‑78 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCgaga                                    >  2:264457/1‑106 (MQ=255)
                                                                                                                                aCCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGccacca   >  2:232408/1‑139 (MQ=255)
                                                                                                                                 cccACGGCGCACCAACGGTCAGCGCACCACACATCgg                                                                                                        >  2:825124/1‑37 (MQ=255)
                                                                                                                                 cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATAc                                                                                           <  1:1179635/50‑1 (MQ=255)
                                                                                                                                 cccACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                              <  1:567349/111‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCA‑GGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAG  >  NC_000913/2085698‑2085964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑