Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,597	G→A	*240* (TAG→TAA)	sanA →	DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,597	0	G	A	100.0%	108.7 / NA	35	*240* (TAG→TAA)	sanA	DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723                                                                                                                                       |                                                                                                                               tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            >  2:551664/1‑139 (MQ=255)          gAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAca                                                                                                                   >  1:893999/1‑139 (MQ=255)                          ggCCGCTGGTCCATATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAATTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGtt                                                                                                   <  1:877325/139‑1 (MQ=255)                            ccGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 <  2:1077411/139‑1 (MQ=255)                             cGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGcc                                                                                                >  1:1141771/1‑139 (MQ=255)                                            cGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                 <  2:680714/139‑1 (MQ=255)                                            cGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                 <  2:1141771/139‑1 (MQ=255)                                              gCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATccc                                                                               >  2:1150825/1‑139 (MQ=255)                                                 aTGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGat                                                                            >  2:988085/1‑139 (MQ=255)                                                    cACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAtt                                                                         >  1:1116439/1‑139 (MQ=255)                                                    cACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCATTGCCGTTTTTTTCCTTATCCCGATAtt                                                                         >  2:818675/1‑139 (MQ=255)                                                              cGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc                                                               >  2:1295858/1‑139 (MQ=255)                                                                   gATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGc                                                          >  1:32147/1‑139 (MQ=255)                                                                    aTGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAtt                                                                         <  1:694143/123‑1 (MQ=255)                                                                    aTGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAtt                                                                         >  2:694143/1‑123 (MQ=255)                                                                     tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCAt                                                        <  1:551664/139‑1 (MQ=255)                                                                       cgcAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATgg                                                      <  2:1116439/139‑1 (MQ=255)                                                                        gcAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGa                                                     <  1:1150825/139‑1 (MQ=255)                                                                                    cgccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgcgcg                                         <  1:1295858/139‑1 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCa                                       >  1:32715/1‑139 (MQ=255)                                                                                          cacaCCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                   <  1:988085/139‑1 (MQ=255)                                                                                                   aCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGa                                                                             <  1:450619/88‑1 (MQ=255)                                                                                                   aCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGa                                                                             >  2:450619/1‑88 (MQ=255)                                                                                                     aGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa                        <  2:1346313/139‑1 (MQ=255)                                                                                                        tACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCt                                                                                     <  1:220586/75‑1 (MQ=255)                                                                                                        tACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCt                                                                                     >  2:220586/1‑75 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAg                                                           >  2:107845/1‑92 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAg                                                           <  1:107845/92‑1 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt            >  1:922068/1‑139 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt            >  2:1186905/1‑139 (MQ=255)                                                                                                                  aCAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCACAGGCGCGATTGATAAACGGGTTAAAAATg           <  2:42217/139‑1 (MQ=255)                                                                                                                      aaGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAcc       <  2:32715/139‑1 (MQ=255)                                                                                                                        gAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCgg     >  2:385597/1‑139 (MQ=255)                                                                                                                          aaCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGaa   <  1:1186905/139‑1 (MQ=255)                                                                                                                           aCAAGGAAAGTCATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc  <  2:893999/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑