Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,350,162	A→G	L284L (TTG→CTG)	glpQ ←	periplasmic glycerophosphodiester phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,350,162	0	A	G	100.0%	168.9 / NA	48	L284L (TTG→CTG)	glpQ	periplasmic glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

TGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGT  >  NC_000913/2350026‑2350293                                                                                                                                         |                                                                                                                                    tGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCa                                                                                                                                   <  2:907473/139‑1 (MQ=255)   aTTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCata                                                                                                                                 >  1:1001531/1‑139 (MQ=255)   aTTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGATTATTCTGCTCAGCATCTTGCACCATGCCAGTGAGTTTGATATTAACCGGCTGCGATGTCTCCTCAATCAGCata                                                                                                                                 >  1:607699/1‑139 (MQ=255)           cAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                         <  2:1001531/139‑1 (MQ=255)              gAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCgg                                                                                                                      <  2:161524/139‑1 (MQ=255)                   tATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCaa                                                                                                                 >  1:1174127/1‑139 (MQ=255)                    aTTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                <  1:204144/139‑1 (MQ=255)                        aGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                            >  1:1079771/1‑139 (MQ=255)                        aGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCa                                                                                                            >  1:713068/1‑139 (MQ=255)                                      aCCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCg                                                                                                                       <  1:1016639/114‑1 (MQ=255)                                      aCCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCg                                                                                                                       >  2:1016639/1‑114 (MQ=255)                                          cACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                          >  2:122918/1‑139 (MQ=255)                                          cACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                          <  2:1091717/139‑1 (MQ=255)                                                       tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                             <  1:796645/139‑1 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                <  2:933101/102‑1 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                >  1:933101/1‑102 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                           <  1:462561/123‑1 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                           >  2:462561/1‑123 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                           >  2:600742/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                           >  1:779210/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                           >  1:599587/1‑139 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                             >  2:1040103/1‑137 (MQ=255)                                                         cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                             <  1:1040103/137‑1 (MQ=255)                                                                          gctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCAGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTc                                                          <  2:891163/139‑1 (MQ=255)                                                                            tgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGt                                                        >  1:554799/1‑139 (MQ=255)                                                                                cATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGtt                                                    <  2:713068/139‑1 (MQ=255)                                                                                cATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGtt                                                    <  1:674322/139‑1 (MQ=255)                                                                                   cTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                <  1:1315144/76‑1 (MQ=255)                                                                                   cTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                >  2:1315144/1‑76 (MQ=255)                                                                                            tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                        >  2:248577/1‑139 (MQ=255)                                                                                            tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                        >  1:989431/1‑139 (MQ=255)                                                                                            tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                        >  2:177048/1‑139 (MQ=255)                                                                                              ccAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                               >  1:1366749/1‑82 (MQ=255)                                                                                              ccAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                               <  2:1366749/82‑1 (MQ=255)                                                                                                  tGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                         >  2:154105/1‑52 (MQ=255)                                                                                                  tGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                         <  1:154105/52‑1 (MQ=255)                                                                                                      tttGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGAc                                                                                                                    >  2:909344/1‑53 (MQ=255)                                                                                                      tttGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGAc                                                                                                                    <  1:909344/53‑1 (MQ=255)                                                                                                                cccGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                         <  1:696056/102‑1 (MQ=255)                                                                                                                cccGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                         >  2:696056/1‑102 (MQ=255)                                                                                                                   ggCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTctgct                 <  1:1356361/139‑1 (MQ=255)                                                                                                                     cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                <  2:876781/42‑1 (MQ=255)                                                                                                                     cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAAt                                                                                                                >  1:876781/1‑42 (MQ=255)                                                                                                                     cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                           <  1:203288/63‑1 (MQ=255)                                                                                                                     cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                           >  2:203288/1‑63 (MQ=255)                                                                                                                             tctcCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                               >  2:756907/1‑51 (MQ=255)                                                                                                                             tctcCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCg                                                                                               <  1:756907/51‑1 (MQ=255)                                                                                                                                 ctcAATCAGCATATGGTAATCCGTACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCTCCCGGGTTAAACATCCGGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAg   <  2:607699/139‑1 (MQ=255)                                                                                                                                  tcaatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGt  <  2:554799/139‑1 (MQ=255)                                                                                                                                  tcaatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGt  <  2:1174127/139‑1 (MQ=255)                                                                                                                                    aatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCAt                                                                                  >  1:1108029/1‑57 (MQ=255)                                                                                                                                    aatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCAt                                                                                  <  2:1108029/57‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TGATTCACATCAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGT  >  NC_000913/2350026‑2350293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑