| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,441,742 | G→A | intergenic (‑137/‑22) | fabB ← / → mnmC | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,441,742 | 0 | G | A | 94.7% | 110.5 / ‑0.4 | 38 | intergenic (‑137/‑22) | fabB/mnmC | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/15); minor base . (1/1); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
CCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAG > NC_000913/2441612‑2441874 | ccTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAg < 2:1177014/139‑1 (MQ=255) ctGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACg > 1:1093436/1‑139 (MQ=255) tAAGTCGCACATAGAGTAAGTTTCGAATGCATAATAGCGTGCACTGGTACGGTGAACAAGTCCGATCAGCCACTTAATAGAGAAATTTGCGCAGCGTTACACACATCGCTAAGAGCGATCCACCACCTGTAAGACGAGt > 1:1100906/1‑139 (MQ=255) tAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGt > 2:794850/1‑139 (MQ=255) aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTCACTTACgcg > 1:805954/1‑137 (MQ=255) aTAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTg < 2:96490/139‑1 (MQ=255) tAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGa < 2:1093436/139‑1 (MQ=255) gagTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGaaa < 1:510182/139‑1 (MQ=255) gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac > 2:739966/1‑134 (MQ=255) gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACactac < 1:739966/134‑1 (MQ=255) gAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCAt > 2:1339521/1‑139 (MQ=255) tACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCt > 2:1066948/1‑139 (MQ=255) gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta > 2:1274579/1‑111 (MQ=255) gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta < 1:1274579/111‑1 (MQ=255) gTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAAttt > 2:625911/1‑139 (MQ=255) cGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCA‑‑‑CCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt > 2:192224/1‑139 (MQ=255) cGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCt < 1:775297/139‑1 (MQ=255) aCAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCA‑‑‑CCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGt < 1:192224/139‑1 (MQ=255) cGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCa > 2:566625/1‑112 (MQ=255) cGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCa < 1:566625/112‑1 (MQ=255) cAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGt > 1:436795/1‑139 (MQ=255) aaTAGAGAAATTTGCGCAGCCCTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTTCGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCCGTTTcctggga < 1:794850/139‑6 (MQ=255) gagaAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCGGAATtttt > 1:1329194/1‑139 (MQ=255) aTCGCTAATATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta < 1:966507/53‑1 (MQ=255) aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACacta > 2:966507/1‑53 (MQ=255) aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 2:200721/1‑139 (MQ=255) aTCGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 1:314567/1‑139 (MQ=255) aTCGCTAAGATCGAGCCACCACCTGTAAAACGAGAAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATa > 1:885767/1‑139 (MQ=255) cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATg < 2:1132503/119‑1 (MQ=255) cGCTAAGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATg > 1:1132503/1‑119 (MQ=255) aaGATCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGc < 2:436795/139‑1 (MQ=255) tCGAGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGa > 1:382724/1‑139 (MQ=255) aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGa > 1:1241720/1‑64 (MQ=255) aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGa < 2:1241720/64‑1 (MQ=255) aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga > 1:1081122/1‑139 (MQ=255) aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga > 1:244766/1‑139 (MQ=255) aGCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAaga < 1:1339521/139‑1 (MQ=255) gCCACCACCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAagag < 1:200721/139‑1 (MQ=255) | CCTCTGTAAGTCGCACATAGAGTAAGTTTCGAATGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAG > NC_000913/2441612‑2441874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |