Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,228 C→T *274* (TAG→TAA yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2280CT100.0% 78.8 / NA 26*274* (TAG→TAAyfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

GCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAC  >  NC_000913/2449093‑2449359
                                                                                                                                       |                                                                                                                                   
gCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTAc                                                                                                                                  <  1:299873/139‑1 (MQ=255)
            cgTTGTTGGGGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGCGGAGTTGAGCGGCTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATTTAAGGGTTACATCTCATTCAGt                                                                                                                      <  1:282847/139‑1 (MQ=255)
                         gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAAcc                                                                                                         >  1:122849/1‑139 (MQ=255)
                          attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACaa                                                                                                                                <  1:809248/115‑1 (MQ=255)
                          attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACaa                                                                                                                                >  2:809248/1‑115 (MQ=255)
                                                 gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAt                                                                                                                           <  2:452360/97‑1 (MQ=255)
                                                 gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAt                                                                                                                           >  1:452360/1‑97 (MQ=255)
                                                   cgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                               <  1:749451/139‑1 (MQ=255)
                                                                    gTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                              <  1:1340671/139‑1 (MQ=255)
                                                                      tGAGCGCGTTGCGGCAGGTACGGGTAATGGGAGGGGCGAATGCCCCTGCCTGGTGTATCGAAGGGTTACAACGCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                            <  2:1346691/139‑1 (MQ=255)
                                                                                              tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                    <  2:432881/139‑1 (MQ=255)
                                                                                                      ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                            <  2:225688/139‑1 (MQ=255)
                                                                                                       gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCg                                                                                                        >  2:82917/1‑62 (MQ=255)
                                                                                                       gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCg                                                                                                        <  1:82917/62‑1 (MQ=255)
                                                                                                       gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAg                           >  2:129296/1‑139 (MQ=255)
                                                                                                         gCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                            <  1:128678/136‑1 (MQ=255)
                                                                                                         gCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                            >  2:128678/1‑136 (MQ=255)
                                                                                                            aaTGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAtt                                                                                                                          <  1:640492/39‑1 (MQ=255)
                                                                                                            aaTGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCAtt                                                                                                                          >  2:640492/1‑39 (MQ=255)
                                                                                                            aaTGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCg                                 <  1:201253/128‑1 (MQ=255)
                                                                                                            aaTGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCg                                 >  2:201253/1‑128 (MQ=255)
                                                                                                                   ctgcctgGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGAAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTg               >  1:971096/1‑139 (MQ=255)
                                                                                                                        tgGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTg          <  2:971096/139‑1 (MQ=255)
                                                                                                                             tATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                      >  2:256380/1‑58 (MQ=255)
                                                                                                                             tATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                      <  1:256380/58‑1 (MQ=255)
                                                                                                                                cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc  >  1:343206/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAC  >  NC_000913/2449093‑2449359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: