Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,092	A→G	V82V (GTA→GTG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,092	0	A	G	100.0%	169.6 / NA	50	V82V (GTA→GTG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

GCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATT  >  NC_000913/2477956‑2478224                                                                                                                                         |                                                                                                                                     gCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTc                                                                                                                                    >  2:1136604/1‑139 (MQ=255)        cGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGcc                                                                                                                             <  1:281092/139‑1 (MQ=255)                      tgGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTgg                                                                                                               <  1:434520/139‑1 (MQ=255)                                gCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACtt                                                                                                     <  1:896942/139‑1 (MQ=255)                                             gTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                        <  1:1353919/139‑1 (MQ=255)                                                 aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                          >  2:517152/1‑133 (MQ=255)                                                 aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                    <  2:194523/139‑1 (MQ=255)                                                 aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                    >  1:56376/1‑139 (MQ=255)                                                 aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAACTGGTCTGACACTTCAACGCTGCCg                                                                                          <  1:517152/133‑1 (MQ=255)                                                  tGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCtt                                                                                   >  1:83496/1‑139 (MQ=255)                                                      aTTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAg                                                                               >  1:109631/1‑139 (MQ=255)                                                       ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                              >  1:482957/1‑139 (MQ=255)                                                          aaaGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                           <  1:631978/139‑1 (MQ=255)                                                                 aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAtt                                                                    >  2:654706/1‑139 (MQ=255)                                                                 aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAtt                                                                    >  2:570521/1‑139 (MQ=255)                                                                  aTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                   <  2:83496/139‑1 (MQ=255)                                                                    tGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATg                                                                 <  1:900664/139‑1 (MQ=255)                                                                           aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                            >  1:65891/1‑73 (MQ=255)                                                                           aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCg                                                                                                                            <  2:65891/73‑1 (MQ=255)                                                                           aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                        >  2:249808/1‑77 (MQ=255)                                                                           aCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCaga                                                                                                                        <  1:249808/77‑1 (MQ=255)                                                                            cGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAt                                                                     >  1:596018/1‑127 (MQ=255)                                                                            cGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCAt                                                                     <  2:596018/127‑1 (MQ=255)                                                                                              cGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTc                                                                                                             >  1:743153/1‑69 (MQ=255)                                                                                              cGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTc                                                                                                             <  2:743153/69‑1 (MQ=255)                                                                                              cGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCAt                                       <  2:482957/139‑1 (MQ=255)                                                                                                  tATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                   <  2:832736/139‑1 (MQ=255)                                                                                                    tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                 >  1:1192070/1‑139 (MQ=255)                                                                                                    tCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                 <  2:1192070/139‑1 (MQ=255)                                                                                                          ttGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                           >  2:759099/1‑91 (MQ=255)                                                                                                          ttGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                           <  1:759099/91‑1 (MQ=255)                                                                                                           tGGCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgtt                          >  2:10486/1‑139 (MQ=255)                                                                                                             gCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                   <  2:156257/128‑1 (MQ=255)                                                                                                             gCACGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                   >  1:156257/1‑128 (MQ=255)                                                                                                               aCGATACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAg                      <  2:56376/139‑1 (MQ=255)                                                                                                                  aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                        <  2:536481/70‑1 (MQ=255)                                                                                                                  aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                        >  1:536481/1‑70 (MQ=255)                                                                                                                  aTACTGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTgg                   >  1:279741/1‑139 (MQ=255)                                                                                                                      tGGGAAAAATGATGGAAGTGTCCGGGGCCGTAGAAAGAATTGGTCTGa                                                                                                          <  2:856374/48‑1 (MQ=255)                                                                                                                      tGGGAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGa                                                                                                          >  1:856374/1‑48 (MQ=255)                                                                                                                         gAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                                    >  2:56524/1‑115 (MQ=255)                                                                                                                         gAAAAATGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCAc                                    <  1:56524/115‑1 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                         <  1:545435/58‑1 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGc                                                                                         >  2:545435/1‑58 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                           >  1:1346833/1‑104 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                           <  2:1346833/104‑1 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                   >  1:1309695/1‑112 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                   <  2:1309695/112‑1 (MQ=255)                                                                                                                             aaTGATGGAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTa        >  2:653827/1‑139 (MQ=255)                                                                                                                                   ggAAGTGTCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGAtt  >  1:1286980/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     GCTTCTTCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATT  >  NC_000913/2477956‑2478224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑