Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,694	T→C	intergenic (‑292/‑4)	yfdX ← / → ypdI	uncharacterized protein/putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,694	0	T	C	100.0%	156.8 / NA	46	intergenic (‑292/‑4)	yfdX/ypdI	uncharacterized protein/putative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/19);  total (27/19)

CCTGTCGCAGAGAACTATTCTTATATACCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTTTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCTATAAAGAGACTGAAAAA  >  NC_000913/2494563‑2494817                                                                                                                                    |                                                                                                                            ccTGTCGCAGAGAACTATTCTTATATACCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGa                                                                                                                      >  1:1199004/1‑139 (MQ=255)   tGTCGCAGAGAACTATTCTTATATACCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGaaa                                                                                                                    >  1:884209/1‑139 (MQ=255)     tCGCAGAGAACTATTCTTATATACCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGt                                                                                                                  <  2:884209/139‑1 (MQ=255)                       atataCCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAg                                                                                                >  2:657501/1‑139 (MQ=255)                          taCCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCtt                                                                                             >  1:415983/1‑139 (MQ=255)                          taCCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCtt                                                                                             <  2:945552/139‑1 (MQ=255)                               caAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTAtttt                                                                                        >  2:246937/1‑139 (MQ=255)                                aaaGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTAttttt                                                                                       >  2:381777/1‑139 (MQ=255)                                 aaGAGATGTCATTTATTATATTGATGTAAATACTGAATAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTa                                                                                      <  2:746709/139‑1 (MQ=255)                                 aaGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTa                                                                                      <  2:415983/139‑1 (MQ=255)                                  agagATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTAt                                                                                     <  2:392039/139‑1 (MQ=255)                                        gTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGtct                                                                               <  1:246937/139‑1 (MQ=255)                                                 tataTCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATgg                                                                      >  2:506464/1‑139 (MQ=255)                                                        aTGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCtatt                                                                          >  2:204751/1‑128 (MQ=255)                                                        aTGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCtatt                                                                          <  1:204751/128‑1 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAACCTTAATAATTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCATTGTTTTTTCAt                                                    >  1:727893/1‑139 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAg                                                                                                >  2:175248/1‑95 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAg                                                                                                <  1:175248/95‑1 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTAtt                                                                                    <  2:1312698/107‑1 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTAtt                                                                                    >  1:1312698/1‑107 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCAt                                                    >  1:1232873/1‑139 (MQ=255)                                                                   gATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGCAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCAt                                                    >  1:982066/1‑139 (MQ=255)                                                                    attaattaAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGt                                                                                                                  >  1:54449/1‑76 (MQ=255)                                                                    attaattaAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGt                                                                                                                  <  2:54449/76‑1 (MQ=255)                                                                    attaattaAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCAtt                                                   >  2:624981/1‑139 (MQ=255)                                                                     ttaattaaATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCAttt                                                  <  1:657501/139‑1 (MQ=255)                                                                                  aaTACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTATTACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGggtg                                     >  2:881881/1‑139 (MQ=255)                                                                                        gCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGt                                                                                 >  2:1169736/1‑89 (MQ=255)                                                                                        gCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGt                                                                                 <  1:1169736/89‑1 (MQ=255)                                                                                         ccATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGaa                               >  1:956881/1‑138 (MQ=255)                                                                                         ccATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGaa                               <  2:956881/138‑1 (MQ=255)                                                                                          cATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCaa                                                               >  1:38140/1‑105 (MQ=255)                                                                                          cATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCaa                                                               <  2:38140/105‑1 (MQ=255)                                                                                               cATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGagag                        <  1:381777/139‑1 (MQ=255)                                                                                                  cGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGt                                                                                 >  1:1190074/1‑79 (MQ=255)                                                                                                  cGTAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGt                                                                                 <  2:1190074/79‑1 (MQ=255)                                                                                                    tAGGTTCTCAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCt                   >  2:398904/1‑139 (MQ=255)                                                                                                           tcAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGtc                                                                                <  2:1258792/71‑1 (MQ=255)                                                                                                           tcAGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGtc                                                                                >  1:1258792/1‑71 (MQ=255)                                                                                                             aGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCTATAAAgaga          >  2:606304/1‑139 (MQ=255)                                                                                                             aGTGAACCATTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCTATAAAgaga          >  2:252244/1‑139 (MQ=255)                                                                                                                     aTTTTCAGGATAACTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCTATAAAGAGACTGaaaaa  >  2:1210102/1‑139 (MQ=255)                                                                                                                                aaCTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGagag                        <  2:824696/106‑1 (MQ=255)                                                                                                                                aaCTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGagag                        >  1:946152/1‑106 (MQ=255)                                                                                                                                aaCTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGagag                        >  1:824696/1‑106 (MQ=255)                                                                                                                                aaCTCTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGagag                        <  2:946152/106‑1 (MQ=255)                                                                                                                                    |                                                                                                                            CCTGTCGCAGAGAACTATTCTTATATACCACAAAGAGATGTCATTTATTATATCGATGTAAATACTGATTAATTAAATGTAAATACAGCCATAACATCGTAGGTTCTCAGTGAACCATTTTCAGGATAACTTTCTATGAAAGTAAACTTAATACTTTTCAGCTTATTTTTATTGGTCTCTATTATGGCATGCAATGTTTTTGCATTTTCCATTTCGGGTGGTGGAAGTGAGAGGAGCTATAAAGAGACTGAAAAA  >  NC_000913/2494563‑2494817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑