| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,518,271 | G→A | intergenic (‑40/‑196) | alaW ← / → yfeC | tRNA‑Ala/DUF1323 family putative DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,518,271 | 0 | G | A | 100.0% | 134.5 / NA | 42 | intergenic (‑40/‑196) | alaW/yfeC | tRNA‑Ala/DUF1323 family putative DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20) | |||||||||||
CTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTG > NC_000913/2518142‑2518404 | cTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGcc > 1:1211721/1‑139 (MQ=255) gTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGaa > 1:309743/1‑139 (MQ=255) tGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGc < 1:1187354/133‑1 (MQ=255) tGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGc > 2:1187354/1‑133 (MQ=255) ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCaa > 2:384687/1‑139 (MQ=255) cTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTg > 1:665331/1‑139 (MQ=255) gggATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgt < 1:384687/139‑1 (MQ=255) aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGttt > 2:912108/1‑139 (MQ=255) cTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtg < 2:1267035/128‑1 (MQ=255) cTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtg > 1:1267035/1‑128 (MQ=255) tGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCg < 2:1211721/139‑1 (MQ=255) gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAgg < 1:432080/139‑1 (MQ=255) tgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCaaa > 1:432657/1‑139 (MQ=255) aGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGaa < 2:432657/139‑1 (MQ=255) gCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGaaa > 2:266702/1‑139 (MQ=255) tataGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTc > 2:318467/1‑139 (MQ=255) cccGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTc < 2:665331/139‑1 (MQ=255) cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATgtgt > 2:827531/1‑59 (MQ=255) cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATgtgt < 1:827531/59‑1 (MQ=255) cGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCag < 1:1094836/139‑1 (MQ=255) gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt > 2:1030000/1‑139 (MQ=255) gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt > 1:175356/1‑139 (MQ=255) gTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCagt < 2:309743/139‑1 (MQ=255) gTAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTa > 2:580691/1‑139 (MQ=255) tAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtgt > 2:542855/1‑73 (MQ=255) tAAAGCTTGTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACgtgt < 1:542855/73‑1 (MQ=255) gTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCa < 1:1327878/59‑1 (MQ=255) gTCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCa > 2:1327878/1‑59 (MQ=255) tCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCAc > 2:967968/1‑139 (MQ=255) tCGAGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCAc > 1:153913/1‑139 (MQ=255) aGTTGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGt < 1:434220/139‑1 (MQ=255) tGACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTc < 2:175356/139‑1 (MQ=255) gACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTc > 1:786602/1‑125 (MQ=255) gACGGGCGGCATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTc < 2:786602/125‑1 (MQ=255) ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg < 1:580691/139‑1 (MQ=255) ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg < 1:266702/139‑1 (MQ=255) ggcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAg > 1:257568/1‑139 (MQ=255) gcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGc < 2:1152086/74‑1 (MQ=255) gcATCATATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGc > 1:1152086/1‑74 (MQ=255) catcatATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtg < 1:318467/139‑1 (MQ=255) tcatATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtggt > 2:121459/1‑139 (MQ=255) catATAAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAgtggtg < 2:153913/139‑1 (MQ=255) | CTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCCCGAATGTGTCAACGGCAAATTGCAACGTGTAGTTTCAATCGCTGAAAAATCAGGCAAATGAACAATTTTGGAAAACCGCTCGCATTCAGTAGTTATTCATGTCACGGTTTCCTGTAAAGTGGTG > NC_000913/2518142‑2518404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |