| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,534,031 | T→C | intergenic (+10/‑35) | ptsH → / → ptsI | phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,534,031 | 0 | T | C | 100.0% | 175.4 / NA | 50 | intergenic (+10/‑35) | ptsH/ptsI | phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/28); total (22/28) | |||||||||||
GAAAAGCCTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTG > NC_000913/2533895‑2534162 | gAAAAGCCTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTc < 2:934131/139‑1 (MQ=255) cTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑aaa < 2:1143549/139‑1 (MQ=255) aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAAATCAGt < 1:728646/139‑1 (MQ=255) aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAAt > 1:142720/1‑134 (MQ=255) aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAAt < 2:142720/134‑1 (MQ=255) aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTc < 2:390134/139‑1 (MQ=255) aGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAg < 2:775461/139‑1 (MQ=255) ctGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAgg < 2:427251/139‑1 (MQ=255) tGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt < 1:403964/139‑1 (MQ=255) aCTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAACCAGTc > 2:991750/1‑120 (MQ=255) aCTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAACCAGTc < 1:991750/120‑1 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACaa > 2:1192923/1‑114 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACaa < 1:1192923/114‑1 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAg < 1:911839/115‑1 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAg > 2:911839/1‑115 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAgg < 2:191710/138‑1 (MQ=255) ccGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAgg > 1:191710/1‑138 (MQ=255) aCTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTACGGt > 1:1332353/1‑113 (MQ=255) aCTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt < 2:1332353/113‑1 (MQ=255) gAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTc < 1:1216646/84‑1 (MQ=255) gAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTc > 2:1216646/1‑84 (MQ=255) cGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTa > 1:945677/1‑91 (MQ=255) cGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTa < 2:945677/91‑1 (MQ=255) aGCAGAAAGCGGTTGAACATCTGGTTAAATTGATGGCGGAACTCGGGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTa > 1:789674/1‑112 (MQ=255) aGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAg < 2:1155141/82‑1 (MQ=255) aGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAg > 1:1155141/1‑82 (MQ=255) aGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTa < 2:789674/112‑1 (MQ=255) aGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTaaa > 1:1159809/1‑139 (MQ=255) aGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGct < 2:733920/139‑1 (MQ=255) cATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAaaga < 2:811168/139‑1 (MQ=255) tCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt < 2:524243/69‑1 (MQ=255) tCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt > 1:524243/1‑69 (MQ=255) aaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAgg > 2:199563/1‑64 (MQ=255) aaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAgg < 1:199563/64‑1 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTc > 2:978565/1‑44 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTc < 1:978565/44‑1 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt > 1:1286985/1‑55 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGt < 2:1286985/55‑1 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGct < 2:247485/109‑1 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGct > 1:247485/1‑109 (MQ=255) tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCAtt > 1:857451/1‑139 (MQ=255) gAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACaa < 2:576828/43‑1 (MQ=255) gAACTCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACaa > 1:576828/1‑43 (MQ=255) tCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGt < 1:944606/41‑1 (MQ=255) tCGAGTAATTTCCCGGGCTCTTTT‑AAAAATCAGTCACAAGt > 2:944606/1‑41 (MQ=255) tCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTg > 2:565151/1‑139 (MQ=255) tCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGCAAAGct > 1:260573/1‑90 (MQ=255) tCCCGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGCAAAGct < 2:260573/90‑1 (MQ=255) cGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCAtt > 1:950778/1‑117 (MQ=255) cGGGCTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCAtt < 2:950778/117‑1 (MQ=255) | GAAAAGCCTGTTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTT‑AAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTG > NC_000913/2533895‑2534162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |