| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 247,461 | G→A | *250* (TAG→TAA) | yafL → | putative lipoprotein and C40 family peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 247,461 | 0 | G | A | 97.5% | 114.0 / NA | 40 | *250* (TAG→TAA) | yafL | putative lipoprotein and C40 family peptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/17); minor base T (0/1); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAA > NC_000913/247325‑247584 | tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa > 2:147568/1‑139 (MQ=255) tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACg > 2:1136366/1‑139 (MQ=255) aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGtt > 1:1363969/1‑139 (MQ=255) ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAc > 2:447884/1‑139 (MQ=255) aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgccg < 1:223947/139‑1 (MQ=255) ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa < 1:1136366/139‑1 (MQ=255) ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa < 2:39955/139‑1 (MQ=255) aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa < 2:1329923/139‑1 (MQ=255) aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa > 1:1046833/1‑139 (MQ=255) aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa > 2:1202673/1‑139 (MQ=255) tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa > 1:832786/1‑139 (MQ=255) aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGCCAGTTGTACGCAGGTGCCTGATGCGACGCTgg > 1:701810/1‑139 (MQ=255) aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa > 2:269864/1‑101 (MQ=255) aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCa < 1:269864/101‑1 (MQ=255) aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc < 1:591443/139‑1 (MQ=255) gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg > 2:842328/1‑126 (MQ=255) gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg < 1:842328/126‑1 (MQ=255) tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg > 1:954678/1‑139 (MQ=255) tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc < 1:42664/87‑1 (MQ=38) tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc > 2:42664/1‑87 (MQ=38) tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg < 2:954678/139‑1 (MQ=255) gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc < 1:755299/83‑1 (MQ=37) gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc > 2:755299/1‑83 (MQ=37) cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc < 2:169601/106‑1 (MQ=255) cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc > 1:169601/1‑106 (MQ=255) cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc > 2:1157185/1‑139 (MQ=255) aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc < 2:1363969/139‑1 (MQ=255) aTTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGa < 1:1202673/139‑1 (MQ=255) ttGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg > 1:672032/1‑52 (MQ=21) ttGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg < 2:672032/52‑1 (MQ=21) gaggagaCGATTTTGTATGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg < 1:354383/66‑1 (MQ=18) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg < 2:1004591/46‑1 (MQ=17) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg > 1:1004591/1‑46 (MQ=17) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg > 2:354383/1‑69 (MQ=21) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg > 2:778243/1‑69 (MQ=21) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg < 1:778243/69‑1 (MQ=25) gAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGGCGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca > 1:1265773/1‑139 (MQ=255) aaGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaa > 2:1028671/1‑139 (MQ=255) tGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg < 1:27735/43‑1 (MQ=14) tGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg > 2:27735/1‑43 (MQ=14) | TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAA > NC_000913/247325‑247584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |