Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,574,135 G→A D57D (GAC→GAT eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,574,1350GA100.0% 92.8 / NA 30D57D (GAC→GATeutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

CATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGA  >  NC_000913/2574000‑2574259
                                                                                                                                       |                                                                                                                            
cATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTtcatcatca                                                                                                                           >  1:1088244/1‑139 (MQ=255)
                 ggTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCGTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                          <  2:1302183/139‑1 (MQ=255)
                   tGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                        <  2:133829/139‑1 (MQ=255)
                             tATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATaaa                                                                                              >  1:1282916/1‑139 (MQ=255)
                                                         acacAGTTCGCAGCTCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGTCTACTGCTGTTCATCATCAACAAAAAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                  <  2:837440/139‑1 (MQ=255)
                                                                                gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                          <  1:1098117/76‑1 (MQ=255)
                                                                                gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                          >  2:1098117/1‑76 (MQ=255)
                                                                                gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAg                                           >  2:1307070/1‑139 (MQ=255)
                                                                                gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAg                                           >  2:682198/1‑139 (MQ=255)
                                                                                   gTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAggcgg                                                                                                               >  1:1369816/1‑68 (MQ=255)
                                                                                   gTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAggcgg                                                                                                               <  2:1369816/68‑1 (MQ=255)
                                                                                             aCTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                              <  1:682198/139‑1 (MQ=255)
                                                                                             aCTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                              >  2:217929/1‑139 (MQ=255)
                                                                                              cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                       >  2:731599/1‑65 (MQ=255)
                                                                                              cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                       <  1:731599/65‑1 (MQ=255)
                                                                                                ggTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAggg                           <  1:1307070/139‑1 (MQ=255)
                                                                                                     gCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATg                      <  1:217929/139‑1 (MQ=255)
                                                                                                      cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                       >  2:391801/1‑57 (MQ=255)
                                                                                                      cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                       <  1:391801/57‑1 (MQ=255)
                                                                                                           gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                        <  2:106342/51‑1 (MQ=255)
                                                                                                           gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                        >  1:106342/1‑51 (MQ=255)
                                                                                                               gggCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg                                                                                                                  <  2:1273369/37‑1 (MQ=38)
                                                                                                               gggCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg                                                                                                                  >  1:1273369/1‑37 (MQ=38)
                                                                                                                ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                              <  1:903958/120‑1 (MQ=255)
                                                                                                                ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                              >  2:903958/1‑120 (MQ=255)
                                                                                                                ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTgcgc           >  2:549637/1‑139 (MQ=255)
                                                                                                                    gcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCagag                                                   <  2:1131855/95‑1 (MQ=255)
                                                                                                                    gcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCagag                                                   >  1:1131855/1‑95 (MQ=255)
                                                                                                                         tgctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGa  <  1:549637/139‑1 (MQ=255)
                                                                                                                           ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCggg                                                        <  1:656598/83‑1 (MQ=255)
                                                                                                                           ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCggg                                                        >  2:656598/1‑83 (MQ=255)
                                                                                                                                       |                                                                                                                            
CATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGA  >  NC_000913/2574000‑2574259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: