Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,658,145 T→C Q264R (CAG→CGG)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,658,1450TC100.0% 212.2 / NA 60Q264R (CAG→CGG) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

TACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAA  >  NC_000913/2658012‑2658278
                                                                                                                                     |                                                                                                                                     
tACCAGTGGCGCGATCAGTTCATTGAATTCTTCACGGCTGATTTCGCCCTGCCAGACCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAAcg                                                                                                                                  <  2:1260148/139‑1 (MQ=255)
       ggCGCGATCAGTTCATTGAATTATTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTAt                                                                                                                           >  2:211009/1‑139 (MQ=255)
           cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  1:1226273/139‑1 (MQ=255)
           cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  1:749974/139‑1 (MQ=255)
                  ttCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTAc                                                                                                                     >  2:38775/1‑134 (MQ=255)
                  ttCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTAc                                                                                                                     <  1:38775/134‑1 (MQ=255)
                   tCATTGAATTATTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  1:211009/139‑1 (MQ=255)
                       tGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAAt                                                                                                           >  2:734473/1‑139 (MQ=255)
                             gTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGc                                                                                                     <  1:734473/139‑1 (MQ=255)
                              ttCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGcc                                                                                                    >  1:844679/1‑139 (MQ=255)
                               tCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCt                                                                                                   >  2:340244/1‑139 (MQ=255)
                                        attacGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTa                                                                                                                            >  2:819601/5‑105 (MQ=255)
                                        aTTACGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGtca                                                                                                                            <  1:819601/105‑3 (MQ=255)
                                             gCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTaa                                                                                     <  1:340244/139‑1 (MQ=255)
                                               ccTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  2:867948/103‑1 (MQ=255)
                                               ccTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  1:867948/1‑103 (MQ=255)
                                                  gCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  <  1:1013304/121‑1 (MQ=255)
                                                  gCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  >  2:1013304/1‑121 (MQ=255)
                                                         cGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGa                                                                         <  2:844679/139‑1 (MQ=255)
                                                            aaCGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATgg                                                                      <  1:1257744/139‑1 (MQ=255)
                                                                   aCGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAat                                                               >  1:375426/1‑139 (MQ=255)
                                                                        cACGGAGTGCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGaa                                                                                                                                    >  2:889206/1‑65 (MQ=255)
                                                                        cACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGaa                                                                                                                                    <  1:889206/65‑1 (MQ=255)
                                                                        cACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               <  1:846127/86‑1 (MQ=255)
                                                                        cACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATcg                                                          >  1:33255/1‑139 (MQ=255)
                                                                        cACGGAGTCCGCATCGCTCAGCGCGATTATGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAg                                                                                                               >  2:846127/1‑86 (MQ=255)
                                                                         aCGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATcgc                                                         >  2:642326/1‑139 (MQ=255)
                                                                              gTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGccgccg                                                    <  1:642326/139‑1 (MQ=255)
                                                                              gTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGccgccg                                                    <  2:375426/139‑1 (MQ=255)
                                                                                 cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGa                                                                         <  2:1311592/115‑1 (MQ=255)
                                                                                 cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGa                                                                         >  1:1311592/1‑115 (MQ=255)
                                                                                 cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCgccgcc                                                     >  1:1228068/1‑135 (MQ=255)
                                                                                 cGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCgccgcc                                                     <  2:1228068/135‑1 (MQ=255)
                                                                                        cTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAAt                                          >  2:607137/1‑139 (MQ=255)
                                                                                             cgcgATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCcagca                                                                           >  1:14516/1‑101 (MQ=255)
                                                                                             cgcgATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCcagca                                                                           <  2:14516/101‑1 (MQ=255)
                                                                                                  ttttGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       >  2:302745/1‑52 (MQ=255)
                                                                                                  ttttGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCt                                                                                                                       <  1:302745/52‑1 (MQ=255)
                                                                                                  ttttGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTcg                                                                                              >  2:213887/1‑77 (MQ=255)
                                                                                                  ttttGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTcg                                                                                              <  1:213887/77‑1 (MQ=255)
                                                                                                  ttttGGCTGCAATGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGt                                >  1:436493/1‑139 (MQ=255)
                                                                                                            aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  <  2:399288/63‑1 (MQ=255)
                                                                                                            aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg                                                                                                  >  1:399288/1‑63 (MQ=255)
                                                                                                            aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGc                         >  2:1321118/1‑136 (MQ=255)
                                                                                                            aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGc                         <  1:1321118/136‑1 (MQ=255)
                                                                                                            aaTGGCGGCATCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACt                      >  1:1182881/1‑139 (MQ=255)
                                                                                                                     aTCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGc                             <  1:559618/123‑1 (MQ=255)
                                                                                                                     aTCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGc                             >  2:559618/1‑123 (MQ=255)
                                                                                                                      tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGc                                                                                                     <  2:1228034/50‑1 (MQ=255)
                                                                                                                      tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGc                                                                                                     >  1:1228034/1‑50 (MQ=255)
                                                                                                                      tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGaa                                                                                          >  1:733884/1‑61 (MQ=255)
                                                                                                                      tCCAGCAGTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGaa                                                                                          <  2:733884/61‑1 (MQ=255)
                                                                                                                        cagcagTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGc                                                                                                     <  2:982141/48‑1 (MQ=255)
                                                                                                                        cagcagTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGc                                                                                                     >  1:982141/1‑48 (MQ=255)
                                                                                                                         agcagTTCACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACAcgccgc         >  2:1265857/1‑139 (MQ=255)
                                                                                                                                cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGcc                                                                                                    >  2:473686/1‑41 (MQ=255)
                                                                                                                                cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGcc                                                                                                    <  1:473686/41‑1 (MQ=255)
                                                                                                                                cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAg                                                  >  2:348518/1‑91 (MQ=255)
                                                                                                                                cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAg                                                  <  1:348518/91‑1 (MQ=255)
                                                                                                                                cACGCCGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTaa  >  2:802224/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAA  >  NC_000913/2658012‑2658278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑