Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,673,768	G→A	*141* (TAG→TAA)	yphA →	DoxX family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,673,768	0	G	A	100.0%	116.1 / NA	37	*141* (TAG→TAA)	yphA	DoxX family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

GTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGG  >  NC_000913/2673632‑2673897                                                                                                                                         |                                                                                                                                  gTCACCATTACTGGGATATGACCGGCGATGCGGTTGAGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGa                                                                                                                                 >  2:151454/1‑139 (MQ=255)      cATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGAt                                                                                                                            >  2:1192084/1‑139 (MQ=255)                atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATgcg                                                                                                                  <  1:1338840/139‑1 (MQ=255)                atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATgcg                                                                                                                  >  1:819486/1‑139 (MQ=255)                           gATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                       <  1:390185/139‑1 (MQ=255)                                  ttGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                <  1:1192084/139‑1 (MQ=255)                                                   aaTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGCGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACa                                                                               <  1:864900/139‑1 (MQ=255)                                                     tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                             >  1:1026318/1‑139 (MQ=255)                                                     tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                             <  2:753142/139‑1 (MQ=255)                                                     tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                             >  2:633651/1‑139 (MQ=255)                                                      ttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                                            >  1:998113/1‑107 (MQ=255)                                                      ttCTGGAAGAATGGCAGTATCGCGGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                                            <  2:998113/107‑1 (MQ=255)                                                                      gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                                            <  2:762116/139‑1 (MQ=255)                                                                            cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                       >  1:422382/1‑90 (MQ=255)                                                                            cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGcc                                                                                                       <  2:422382/90‑1 (MQ=255)                                                                            cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                              <  1:321465/99‑1 (MQ=255)                                                                            cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                              >  2:321465/1‑99 (MQ=255)                                                                                 gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg                                                 <  1:712397/139‑1 (MQ=255)                                                                                      cTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttgtt                                            <  1:653220/139‑1 (MQ=255)                                                                                        tGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa                                          <  1:633651/139‑1 (MQ=255)                                                                                         gCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                       <  2:973820/93‑1 (MQ=255)                                                                                         gCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                       >  1:973820/1‑93 (MQ=255)                                                                                               ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt                                   >  2:104232/1‑139 (MQ=255)                                                                                                   aTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGa                                                                                                                      >  1:205577/1‑52 (MQ=255)                                                                                                   aTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGa                                                                                                                      <  2:205577/52‑1 (MQ=255)                                                                                                    ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                    <  1:1219327/101‑1 (MQ=255)                                                                                                    ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                    >  2:1219327/1‑101 (MQ=255)                                                                                                       ccgggccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                           <  1:151454/139‑1 (MQ=255)                                                                                                           gccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAGATTACAGCCACGCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGATGCTAGCGCa                       <  1:145710/139‑1 (MQ=255)                                                                                                            ccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                                            <  2:76984/53‑1 (MQ=255)                                                                                                            ccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGa                                                                                                            >  1:76984/1‑53 (MQ=255)                                                                                                            ccgggGGCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                      <  1:104232/139‑1 (MQ=255)                                                                                                                 ggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                                                   >  1:821508/1‑73 (MQ=255)                                                                                                                 ggCAATTTCTCTCGATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAt                                                                                   <  2:821508/73‑1 (MQ=255)                                                                                                                              gATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                                            >  2:35583/1‑83 (MQ=255)                                                                                                                              gATCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa                                                            <  1:35583/83‑1 (MQ=255)                                                                                                                                tCGGCGTTAAGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCggg  >  2:60625/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  GTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGG  >  NC_000913/2673632‑2673897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑