| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,758,359 | C→T | S81N (AGC→AAC) | yfjH ← | CP4‑57 prophage, uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,758,359 | 0 | C | T | 98.7% | 245.2 / NA | 77 | S81N (AGC→AAC) | yfjH | CP4‑57 prophage, uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (38/38); minor base A (0/1); total (38/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGT > NC_000913/2758237‑2758493 | cTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGaa < 2:417983/130‑1 (MQ=255)cTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGaa > 1:417983/1‑130 (MQ=255) aTCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 2:91004/139‑1 (MQ=255) aTCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 2:798175/139‑1 (MQ=255) aTCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 2:514931/139‑1 (MQ=255) aTCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 1:879746/139‑1 (MQ=255) ttCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGc > 2:632840/1‑139 (MQ=255) tgtATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAAt > 2:288976/1‑139 (MQ=255) tATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGctct < 1:1073806/117‑1 (MQ=255) tATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGctct > 2:1073806/1‑117 (MQ=255) cttcCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt > 1:129847/1‑119 (MQ=255) cttcCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 2:129847/119‑1 (MQ=255) aTCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGgat < 2:1042579/139‑1 (MQ=255) cAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCt < 1:632840/139‑1 (MQ=255) cccATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCctct > 2:920058/1‑139 (MQ=255) ttCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 1:288976/139‑1 (MQ=255) ttCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 2:490563/139‑1 (MQ=255) tGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGt > 2:448544/1‑109 (MQ=255) tGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGt < 1:448544/109‑1 (MQ=255) tATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCt < 2:1140819/99‑1 (MQ=255) tATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCt > 1:1140819/1‑99 (MQ=255) tGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACa > 2:293934/1‑132 (MQ=255) tGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACa < 1:293934/132‑1 (MQ=255) tGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGcc > 2:1315970/1‑89 (MQ=255) tGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGcc < 1:1315970/89‑1 (MQ=255) tGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCctc > 1:989000/1‑122 (MQ=255) tGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCctc < 2:989000/122‑1 (MQ=255) tGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCa > 2:617932/1‑139 (MQ=255) ccTAGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 2:984224/125‑1 (MQ=255) ccTAGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa > 1:984224/1‑125 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCa > 2:1282419/1‑79 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCa < 1:1282419/79‑1 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCt > 1:876213/1‑91 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCt < 2:876213/91‑1 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCa > 1:505860/1‑93 (MQ=255) cTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCa < 2:505860/93‑1 (MQ=255) tttCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATc < 1:455484/109‑1 (MQ=255) tttCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATc > 2:455484/1‑109 (MQ=255) ttCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCcat < 1:617932/139‑1 (MQ=255) aTCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTg > 2:408121/1‑54 (MQ=255) aTCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTg < 1:408121/54‑1 (MQ=255) aTCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGAc < 1:187642/139‑1 (MQ=255) cGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGgat < 2:551423/90‑1 (MQ=255) cGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGgat > 1:551423/1‑90 (MQ=255) gAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 2:599851/100‑1 (MQ=255) gAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa > 1:599851/1‑100 (MQ=255) gAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGAt > 1:1073468/1‑139 (MQ=255) gAATATAACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGAt > 1:875258/1‑139 (MQ=255) tataACCTCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAgg < 2:1073468/139‑1 (MQ=255) tCACACGAGTACCTATTTTCTCAGAGTTCCAGAAt > 2:695001/1‑35 (MQ=255) tCACACGAGTACCTATTTTCTCAGAGTTCCAGAAt < 1:695001/35‑1 (MQ=255) tCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCaa > 1:1265700/1‑60 (MQ=255) tCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCaa < 2:1265700/60‑1 (MQ=255) tCACACGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATc < 2:1260988/139‑1 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTcc > 2:905473/1‑82 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCAc > 2:945938/1‑102 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCAc < 1:945938/102‑1 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCa > 1:1121462/1‑139 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCa > 1:891560/1‑139 (MQ=255) cacaCGAGTACCTATTTTCTCAGAGATCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTcc < 1:905473/82‑1 (MQ=255) tACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa > 2:133759/1‑81 (MQ=255) tACCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 1:133759/81‑1 (MQ=255) aCCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt < 2:152747/37‑1 (MQ=255) aCCTATTTTCTCAGAGTTCCAGAATTGCTCTCGCAtt > 1:152747/1‑37 (MQ=255) aTTTTCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCACGTCGTTTTCTAc > 1:226523/1‑139 (MQ=255) tttCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGa > 1:437986/1‑112 (MQ=255) tttCTCAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGa < 2:437986/112‑1 (MQ=255) tctcAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCctc < 1:1315925/67‑1 (MQ=255) tctcAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCctc > 2:1315925/1‑67 (MQ=255) tctcAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAAt > 1:129892/1‑139 (MQ=255) ctcAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCcattcgcatt < 2:1078352/98‑1 (MQ=255) ctcAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCcattcgcatt > 1:1078352/1‑98 (MQ=255) cAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa > 1:1251121/1‑69 (MQ=255) cAGAGTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGaa < 2:1251121/69‑1 (MQ=255) agagTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCa > 2:391593/1‑37 (MQ=255) agagTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCa < 1:391593/37‑1 (MQ=255) agagTTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGt > 2:990029/1‑139 (MQ=255) | CTGGATCATATAATTCAGTGTATTCTTCCTTTATCTCCAGTTGCCCATTCACTGTATGGTGCCTTGTTTCCCATGGTACATCGCAGGAATATAACCTCACACGAGTACCTATTTTCTCAGAGCTCCAGAATTGCTCTCGCATTCTGCCTAGCTCAATCCGTATTTCCGGATGATCTTCCTCTAGAACATCAGTATCCACCGCCCATTCGCATTCGGACATGTGATAGGCGAGATCACGTCGTTTTCTACAAATTTGT > NC_000913/2758237‑2758493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |