Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,800,475 G→A *110* (TAG→TAA ygaM → putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,4750GA100.0% 130.7 / NA 41*110* (TAG→TAAygaMputative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800344‑2800606
                                                                                                                                   |                                                                                                                                   
gtgcAGCAAGCCGCGCGCGATGCCGTTGGCTCCGCGGATTCTTTTGGTCGTGAGAGACCCTGGTATAGCGTGGCTACAGCAGCTGCGGTAGGTATGTTTCTCGGCGCACTGTTAAGCATGCGCACATCGTAATGCaaca                                                                                                                              >  1:903692/4‑137 (MQ=255)
   cAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATg                                                                                                                           >  2:465397/1‑139 (MQ=255)
                              tGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGa                                                                                                >  1:147853/1‑139 (MQ=255)
                                cgcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAg                                                                                              >  2:857699/1‑139 (MQ=255)
                                 gcgGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                             >  1:589502/1‑139 (MQ=255)
                                                         cccTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAAAACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                     <  2:101930/139‑1 (MQ=255)
                                                                  aGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATc                                                            <  2:589502/139‑1 (MQ=255)
                                                                   gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                           >  2:932294/1‑139 (MQ=255)
                                                                    cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAcgc                                                                                                              >  2:735118/1‑87 (MQ=255)
                                                                    cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAcgc                                                                                                              <  1:735118/87‑1 (MQ=255)
                                                                    cGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                          >  1:273594/1‑139 (MQ=255)
                                                                        ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                      <  1:465397/139‑1 (MQ=255)
                                                                        ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                      >  1:1151165/1‑139 (MQ=255)
                                                                         gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaaa                                                     >  2:140154/1‑139 (MQ=255)
                                                                                 gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTa                                             <  2:740117/139‑1 (MQ=255)
                                                                                  cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCCCaaaaa                                                     >  2:448753/1‑130 (MQ=255)
                                                                                  cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCCCaaaaa                                                     <  1:448753/130‑1 (MQ=255)
                                                                                    gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAccc                                          >  1:733260/1‑139 (MQ=255)
                                                                                       gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                       <  1:145688/139‑1 (MQ=255)
                                                                                         aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  1:932294/139‑1 (MQ=255)
                                                                                              tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCGTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                <  2:903692/139‑1 (MQ=255)
                                                                                                     cGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCa                         <  2:733260/139‑1 (MQ=255)
                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                        >  1:731948/1‑139 (MQ=255)
                                                                                                      ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                        >  1:268356/1‑139 (MQ=255)
                                                                                                        cgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg                      <  2:147853/139‑1 (MQ=255)
                                                                                                                 aaGCATGCACAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGa             <  1:764671/139‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCg                                                                                                 <  1:412689/49‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCg                                                                                                 >  2:412689/1‑49 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCt                                                                                           >  2:347283/1‑55 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCt                                                                                           <  1:347283/55‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                           <  2:1266814/119‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                           >  1:1266814/1‑119 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        >  2:1369862/1‑138 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        <  1:1369862/138‑1 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa       >  2:104924/1‑139 (MQ=255)
                                                                                                                       gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa       >  1:1083598/1‑139 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:273594/139‑1 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:731948/139‑1 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  1:140154/139‑1 (MQ=255)
                                                                                                                        cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  1:857699/139‑1 (MQ=255)
                                                                                                                            aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc  <  2:268356/139‑1 (MQ=255)
                                                                                                                            aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc  <  2:1151165/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800344‑2800606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: