Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,563	G→A	R354H (CGT→CAT)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,563	0	G	A	100.0%	185.5 / NA	58	R354H (CGT→CAT)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

GACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGG  >  NC_000913/262430‑262694                                                                                                                                      |                                                                                                                                    gACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTg                                                                                                                                >  1:164043/1‑139 (MQ=255) gACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTg                                                                                                                                <  1:948632/139‑1 (MQ=255)       gAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACg                                                                                                                          >  1:1264414/1‑139 (MQ=255)        aGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGa                                                                                                                         >  1:449714/1‑139 (MQ=255)               gTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGAt                                                                                                                  <  1:107841/139‑1 (MQ=255)                      gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  1:176107/139‑1 (MQ=255)                                   aaaTCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTaa                                                                                              <  1:1062405/139‑1 (MQ=255)                                     aTCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACg                                                                                            <  2:527874/139‑1 (MQ=255)                                          cAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCctct                                                                                       <  2:735150/139‑1 (MQ=255)                                                       gATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGtt                                                                                                                              <  2:554745/87‑1 (MQ=255)                                                       gATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGtt                                                                                                                              >  1:554745/1‑87 (MQ=255)                                                          gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                             >  2:207227/1‑85 (MQ=255)                                                          gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                             <  1:207227/85‑1 (MQ=255)                                                          gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGc                                                                                           <  2:644734/119‑1 (MQ=255)                                                          gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGc                                                                                           >  1:644734/1‑119 (MQ=255)                                                            cATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGAATCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggc                                                                     >  2:264097/1‑139 (MQ=255)                                                                  ccATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  1:96239/95‑1 (MQ=255)                                                                  ccATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           >  2:96239/1‑95 (MQ=255)                                                                   cATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGt                                                              >  1:1163452/1‑139 (MQ=255)                                                                     tatTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                            >  2:722271/1‑75 (MQ=255)                                                                     tatTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                            <  1:722271/75‑1 (MQ=255)                                                                        tCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                         <  2:164043/139‑1 (MQ=255)                                                                         cGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTc                                                        >  2:1371746/1‑139 (MQ=255)                                                                          gTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCt                                                       <  1:462660/139‑1 (MQ=255)                                                                           tGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  2:1195180/86‑1 (MQ=255)                                                                           tGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           >  1:1195180/1‑86 (MQ=255)                                                                               cacGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTg                                                  >  2:1086732/1‑139 (MQ=255)                                                                                acGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                            <  1:1137448/64‑1 (MQ=255)                                                                                acGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                            >  2:1137448/1‑64 (MQ=255)                                                                                    cacacaACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                             >  2:1292052/1‑139 (MQ=255)                                                                                         aacaCTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  1:477647/72‑1 (MQ=255)                                                                                         aacaCTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           >  2:477647/1‑72 (MQ=255)                                                                                         aacaCTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGc                                        >  1:521454/1‑139 (MQ=255)                                                                                              tCCGATGCGCTCCTGACCCGCGATATGCCCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                     >  1:269363/1‑73 (MQ=255)                                                                                              tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                     <  2:269363/73‑1 (MQ=255)                                                                                              tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAcg                                                                                    <  1:249540/90‑1 (MQ=255)                                                                                              tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAcg                                                                                    >  2:249540/1‑90 (MQ=255)                                                                                                       aTCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaa                          >  1:324750/1‑139 (MQ=255)                                                                                                         ccTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaaaa                        <  1:1371746/139‑1 (MQ=255)                                                                                                             aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  1:1017087/52‑1 (MQ=255)                                                                                                             aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           >  2:1017087/1‑52 (MQ=255)                                                                                                             aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTcc                    >  2:53150/1‑139 (MQ=255)                                                                                                               ccGCGATATGAGCAACGCCCCGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTAGCGGTAGGCACACAAAAACTCCAc                  >  1:1085882/1‑139 (MQ=255)                                                                                                                 gcgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                     >  2:131612/1‑54 (MQ=255)                                                                                                                 gcgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                     <  1:131612/54‑1 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGAt                                                                                                                  <  2:132557/40‑1 (MQ=39)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGAt                                                                                                                  >  1:132557/1‑40 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                             <  1:1216357/45‑1 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                             >  2:1216357/1‑45 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           >  2:412858/1‑47 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                           <  1:412858/47‑1 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                   >  1:365627/1‑135 (MQ=255)                                                                                                                  cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                   <  2:365627/135‑1 (MQ=255)                                                                                                                       tGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                   >  1:865187/1‑82 (MQ=255)                                                                                                                       tGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                   <  2:865187/82‑1 (MQ=255)                                                                                                                            aaCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCaa     <  2:521454/139‑1 (MQ=255)                                                                                                                              cGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATg   >  1:1074563/1‑139 (MQ=255)                                                                                                                               gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATgg  >  1:207104/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    GACGATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGG  >  NC_000913/262430‑262694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑