Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,351	G→A	*230* (TAG→TAA)	mutH →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,351	0	G	A	100.0%	160.2 / NA	50	*230* (TAG→TAA)	mutH	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

GAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970216‑2970476                                                                                                                                        |                                                                                                                              gAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                            <  2:15250/139‑1 (MQ=255) gAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                            <  2:943491/139‑1 (MQ=255)                       cTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTcc                                                                                                      <  1:888672/139‑1 (MQ=255)                        ttACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                     <  2:1197463/139‑1 (MQ=255)                          aCCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:41264/139‑1 (MQ=255)                                    ttGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:977101/129‑1 (MQ=255)                                    ttGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  1:977101/1‑129 (MQ=255)                                                  gAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                     <  2:595845/113‑1 (MQ=255)                                                  gAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                     >  1:595845/1‑113 (MQ=255)                                                               cGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   <  2:757051/134‑1 (MQ=255)                                                               cGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAggg                                                                   >  1:757051/1‑134 (MQ=255)                                                                   gCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGa                                                          >  1:490423/1‑139 (MQ=255)                                                                        gcgGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTa                                                     <  2:48677/139‑1 (MQ=255)                                                                              tttATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCa                                               >  1:458882/1‑139 (MQ=255)                                                                                      aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCt                                                                                                                       <  2:652387/59‑1 (MQ=255)                                                                                      aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCt                                                                                                                       >  1:652387/1‑59 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                            >  1:580342/1‑38 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                            <  2:580342/38‑1 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:1307336/63‑1 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  1:1307336/1‑63 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTgg                       >  1:705964/1‑139 (MQ=255)                                                                                                      gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTgg                       >  1:417549/1‑139 (MQ=255)                                                                                                         cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       <  1:940551/56‑1 (MQ=255)                                                                                                         cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       >  2:940551/1‑56 (MQ=255)                                                                                                         cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  1:261409/60‑1 (MQ=255)                                                                                                         cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  2:261409/1‑60 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       >  1:122045/1‑48 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       <  1:561470/48‑1 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       <  2:122045/48‑1 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       >  1:387877/1‑48 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       <  2:387877/48‑1 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                       >  2:561470/1‑48 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  1:940056/52‑1 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  2:940056/1‑52 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAg                                                           <  2:481748/92‑1 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAg                                                           >  1:481748/1‑92 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCgg            >  1:4300/1‑139 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCgg            >  2:367871/1‑139 (MQ=255)                                                                                                                 gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCgg            >  2:687731/1‑139 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCttt                                                               >  1:871308/1‑83 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTg                                                 >  2:242831/1‑97 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTg                                                 <  1:242831/97‑1 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgc       >  2:445657/1‑139 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgc       >  2:1275922/1‑139 (MQ=255)                                                                                                                      tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGACATATAATTACCGCTTCATTTTTTTGGCAGGGCttt                                                               <  2:871308/83‑1 (MQ=255)                                                                                                                           ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   <  2:558481/42‑1 (MQ=255)                                                                                                                           ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                                   >  1:558481/1‑42 (MQ=255)                                                                                                                           ttCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg  <  1:367871/139‑1 (MQ=255)                                                                                                                                aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCtt                    <  2:46540/116‑1 (MQ=255)                                                                                                                                aTCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCtt                    >  1:46540/1‑116 (MQ=255)                                                                                                                                        |                                                                                                                              GAAAGCAGCGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACG  >  NC_000913/2970216‑2970476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑