Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	30,616	T→C	P322P (CCT→CCC)	carA →	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	30,616	0	T	C	100.0%	170.1 / NA	49	P322P (CCT→CCC)	carA	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGTT  >  NC_000913/30481‑30747                                                                                                                                        |                                                                                                                                    ggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCa                                                                                                                                  <  2:290268/139‑1 (MQ=255)               aGACTGTCAAAATGAAATTTGGTCACCACGGCGACAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCAc                                                                                                                    <  2:428278/139‑1 (MQ=255)                               aTTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTc                                                                                                    >  1:716362/1‑139 (MQ=255)                                 ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                                  <  2:141962/139‑1 (MQ=255)                                      caccacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                                             <  2:156153/139‑1 (MQ=255)                                       accacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                     <  2:984877/114‑1 (MQ=255)                                       accacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                     >  1:984877/1‑114 (MQ=255)                                              cggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAg                                                                                     >  1:38578/1‑139 (MQ=255)                                                              aaaGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCg                                                                     >  2:1186564/1‑139 (MQ=255)                                                                            aaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                                  >  1:430830/1‑96 (MQ=255)                                                                            aaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                                  <  2:430830/96‑1 (MQ=255)                                                                            aaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAAc                                                               <  1:1020672/131‑1 (MQ=255)                                                                            aaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAAc                                                               >  2:1020672/1‑131 (MQ=255)                                                                             aaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCa                                                      <  2:716362/139‑1 (MQ=255)                                                                                          tCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCa                                         >  2:317974/1‑139 (MQ=255)                                                                                           cACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAc                                        >  2:123948/1‑139 (MQ=255)                                                                                            aCCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                                <  1:229312/82‑1 (MQ=255)                                                                                            aCCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                                >  2:229312/1‑82 (MQ=255)                                                                                            aCCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAcc                                       >  2:191241/1‑139 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc                                                                                                                      <  1:726853/58‑1 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTc                                                                                                                      >  2:726853/1‑58 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCa                                                                                 >  2:1181566/1‑95 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCa                                                                                 <  1:1181566/95‑1 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                                     >  2:442838/1‑139 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                                     >  1:676808/1‑139 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                     <  2:849457/57‑1 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                     >  1:849457/1‑57 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATCAATCCCTGTTCGACGGTa                                                                                             <  2:899962/81‑1 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGACACGCATAAATCCCTGTTCGACGGTa                                                                                             >  1:899962/1‑81 (MQ=255)                                                                                                   aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                                <  1:191241/139‑1 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAAt                                                                                                            >  1:96820/1‑61 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAAt                                                                                                            <  2:96820/61‑1 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTg                                                                                                       >  1:1085229/1‑66 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTg                                                                                                       <  2:1085229/66‑1 (MQ=255)                                                                                                        cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATa                                                                  >  1:701325/1‑100 (MQ=255)                                                                                                        cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATa                                                                  <  2:701325/100‑1 (MQ=255)                                                                                                        cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagccagcc                           >  2:565108/1‑139 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                                  <  1:850892/66‑1 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                                  >  2:850892/1‑66 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                                <  1:524796/132‑1 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                                >  2:524796/1‑132 (MQ=255)                                                                                                               ttGTGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTcc                    >  2:648295/1‑139 (MQ=255)                                                                                                               ttGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTcc                    >  1:953963/1‑139 (MQ=255)                                                                                                                      ggACGAAGCAGCATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTa                                                                                        <  1:1207365/64‑1 (MQ=255)                                                                                                                      ggACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTa                                                                                        >  2:1207365/1‑64 (MQ=255)                                                                                                                            aGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTc                                                 <  1:452135/97‑1 (MQ=255)                                                                                                                            aGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTc                                                 >  2:452135/1‑97 (MQ=255)                                                                                                                            aGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGAcgccgcg       <  1:1186564/139‑1 (MQ=255)                                                                                                                                 cATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCgtt  <  1:1079724/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGTT  >  NC_000913/30481‑30747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑