Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,015	G→A	intergenic (‑105/‑100)	nudF ← / → tolC	ADP‑ribose pyrophosphatase/transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,015	0	G	A	100.0%	166.5 / NA	52	intergenic (‑105/‑100)	nudF/tolC	ADP‑ribose pyrophosphatase/transport channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

CGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177886‑3178149                                                                                                                                  |                                                                                                                                       cGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACg                                                                                                                               >  2:271697/1‑139 (MQ=255)      aGGTTGTCTGGCTTAAGCATTGTTAA‑GTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGTCACATTAACGCCCTATGACACGTAACTCCAAcc                                                                                                                         >  2:1304468/1‑139 (MQ=255)                          tGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCt                                                                                                      >  1:665509/1‑139 (MQ=255)                                   ccTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:1327753/126‑1 (MQ=255)                                   ccTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:1327753/1‑126 (MQ=255)                                    cTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATcc                                                                                            >  1:488807/1‑139 (MQ=255)                                      ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTc                                                                                                       <  2:790071/126‑1 (MQ=255)                                      ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  1:130376/1‑139 (MQ=255)                                      ggCACTAATAGTGAATTAAATGTGAATTTAAGCGACGTTTGACTGGCGTTTGAGCAGTCATGTGTTAAATTGATGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTc                                                                                                       >  1:790071/1‑126 (MQ=255)                                        cACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaa                                                                                        <  2:488807/139‑1 (MQ=255)                                         aCTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAAGCTTTTGCAGTAGCGGCTTCTGCTAGAATCCACaat                                                                                       <  1:1304468/139‑1 (MQ=255)                                            aaTAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTCTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataat                                                                                    >  2:1042928/1‑139 (MQ=255)                                            aaTAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAAGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaataat                                                                                    >  2:852860/1‑139 (MQ=255)                                             aTAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          >  2:634762/1‑132 (MQ=255)                                             aTAGTGAATTAAATGTGAATTTCAGCGACATTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                          <  1:634762/132‑1 (MQ=255)                                                gTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTCTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTa                                                                                <  1:1042928/139‑1 (MQ=255)                                                  gAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  1:1237229/1‑111 (MQ=255)                                                  gAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  2:1237229/111‑1 (MQ=255)                                                     ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  1:911707/108‑1 (MQ=255)                                                     ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  2:911707/1‑108 (MQ=255)                                                        aaTGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGa                                                                        <  1:1154269/139‑1 (MQ=255)                                                          tgtgAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATc                                                                      >  2:144731/1‑139 (MQ=255)                                                               aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCt                                                                 <  1:271697/139‑1 (MQ=255)                                                                        aCGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          >  2:1178419/1‑89 (MQ=255)                                                                        aCGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                          <  1:1178419/89‑1 (MQ=255)                                                                        aCGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGc                                                        >  1:535897/1‑139 (MQ=255)                                                                         cGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCt                                                       <  1:310629/139‑1 (MQ=255)                                                                                    cGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAgg                                            >  1:1313368/1‑139 (MQ=255)                                                                                       ttGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAAt                                         <  2:1313368/139‑1 (MQ=255)                                                                                                      ttAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAAtt                          <  2:665509/139‑1 (MQ=255)                                                                                                       tAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGt                                                                                                                <  1:929849/52‑1 (MQ=255)                                                                                                       tAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGt                                                                                                                >  2:929849/1‑52 (MQ=255)                                                                                                        aaaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGc                        >  2:153375/1‑139 (MQ=255)                                                                                                         aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGaa                                          <  1:352213/120‑1 (MQ=255)                                                                                                         aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGaa                                          >  2:352213/1‑120 (MQ=255)                                                                                                             gAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTcccc                   <  2:130376/139‑1 (MQ=255)                                                                                                             gAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTcccc                   >  1:1350922/1‑139 (MQ=255)                                                                                                              aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGaa                                                                                               >  2:793289/1‑62 (MQ=255)                                                                                                              aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGaa                                                                                               <  1:793289/62‑1 (MQ=255)                                                                                                              aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                      <  1:1138077/103‑1 (MQ=255)                                                                                                              aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                      >  2:1138077/1‑103 (MQ=255)                                                                                                                gCACATTAACGCCCTATGACACGTAACGCCAACCttt                                                                                                                      >  1:861150/1‑37 (MQ=255)                                                                                                                gCACATTAACGCCCTATGACACGTAACGCCAACCttt                                                                                                                      <  2:861150/37‑1 (MQ=255)                                                                                                                gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                      <  1:73552/101‑1 (MQ=255)                                                                                                                gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                      >  2:73552/1‑101 (MQ=255)                                                                                                                gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCAtt                >  2:728081/1‑139 (MQ=255)                                                                                                                     ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                              >  1:1235372/1‑40 (MQ=255)                                                                                                                     ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                              <  2:1235372/40‑1 (MQ=255)                                                                                                                        aCGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCgg        >  1:593389/1‑139 (MQ=255)                                                                                                                        aCGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCgg        >  1:1248435/1‑139 (MQ=255)                                                                                                                           cccTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCt     <  1:852860/139‑1 (MQ=255)                                                                                                                              tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAg  >  2:883850/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       CGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAG  >  NC_000913/3177886‑3178149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑