Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,248,939 T→C intergenic (+117/‑30) mzrA → / → yqjC modulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,248,9390TC100.0% 287.9 / NA 79intergenic (+117/‑30)mzrA/yqjCmodulator of EnvZ/OmpR regulon/DUF1090 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/39);  total (40/39)

ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAG  >  NC_000913/3248809‑3249067
                                                                                                                                  |                                                                                                                                
aTCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGa                                                                                                                          >  1:814635/1‑139 (MQ=255)
          aTAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        >  2:206314/1‑131 (MQ=255)
          aTAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        <  1:206314/131‑1 (MQ=255)
           tAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  2:1651/139‑1 (MQ=255)
           tAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  2:1094087/139‑1 (MQ=255)
           tAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  2:327808/139‑1 (MQ=255)
              cTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           <  1:386746/124‑1 (MQ=255)
              cTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           >  2:386746/1‑124 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               >  2:699773/1‑133 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGa                                                                                                               <  1:699773/133‑1 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                         >  1:1339147/1‑139 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                         <  2:1102323/139‑1 (MQ=255)
                 ccTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTc                                                                                                         >  2:85364/1‑139 (MQ=255)
                   tGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       <  2:284164/139‑1 (MQ=255)
                    gTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAg                                                                                                      >  1:209422/1‑139 (MQ=255)
                            aaaCATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTc                                                                                                                             >  1:1371421/1‑108 (MQ=255)
                            aaaCATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTc                                                                                                                             <  2:1371421/108‑1 (MQ=255)
                                  atCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  1:383699/1‑139 (MQ=255)
                                  atCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCa                                                                                        >  1:1075129/1‑139 (MQ=255)
                                   tcactcacTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCAt                                                                                       <  1:85364/139‑1 (MQ=255)
                                       tcactTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCt                                                                                   <  2:640954/139‑1 (MQ=255)
                                         actTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCttt                                                                                 >  1:1156296/1‑139 (MQ=255)
                                            ttGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGc                                                                              >  2:46415/1‑139 (MQ=255)
                                                   tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGt                                                                                                                              >  1:1023992/1‑84 (MQ=255)
                                                   tttCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGt                                                                                                                              <  2:1023992/84‑1 (MQ=255)
                                                     tCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtctct                                                                     >  1:1269481/1‑139 (MQ=255)
                                                         cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATTGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGtt                                                                          >  1:1066042/1‑130 (MQ=255)
                                                         cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATTGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGtt                                                                          <  2:1066042/130‑1 (MQ=255)
                                                         cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           >  1:947993/1‑81 (MQ=255)
                                                         cGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           <  2:947993/81‑1 (MQ=255)
                                                               tGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCtt                                                           <  2:1269481/139‑1 (MQ=255)
                                                                      gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       >  2:214531/1‑88 (MQ=255)
                                                                      gATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       <  1:214531/88‑1 (MQ=255)
                                                                         tAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTa                                                 <  2:216450/139‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTTTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGc                                                                                         <  1:890656/97‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           >  2:904848/1‑63 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCg                                                                                                                           <  1:904848/63‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        <  2:1242180/66‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        >  1:1242180/1‑66 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        <  1:529929/114‑1 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        >  2:529929/1‑114 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTTGTGCCGGTAGt                                               >  2:892647/1‑139 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGTCGGTAGt                                               >  1:46355/1‑139 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                               >  2:291684/1‑139 (MQ=255)
                                                                           aTGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGt                                               >  1:941297/1‑139 (MQ=255)
                                                                           aTGAGGCGATGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGc                                                                                         >  2:890656/1‑97 (MQ=255)
                                                                                 cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        >  1:857164/1‑108 (MQ=255)
                                                                                 cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTtc                                                                        <  2:857164/108‑1 (MQ=255)
                                                                                 cGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGcc                                         >  2:835757/1‑139 (MQ=255)
                                                                                   gtggtTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCAc                                       >  1:1035147/1‑139 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:941297/139‑1 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:918307/139‑1 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  1:46415/139‑1 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  1:835757/139‑1 (MQ=255)
                                                                                       ttAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:383699/139‑1 (MQ=255)
                                                                                         aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       >  2:796404/1‑69 (MQ=255)
                                                                                         aaCACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCaa                                                                                                       <  1:796404/69‑1 (MQ=255)
                                                                                             cTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        >  1:1256287/1‑48 (MQ=255)
                                                                                             cTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAAt                                                                                                                        <  2:1256287/48‑1 (MQ=255)
                                                                                              tACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTc                            >  2:1312962/1‑139 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                               >  1:812792/1‑86 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAg                                                                               <  2:812792/86‑1 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                          >  1:920694/1‑139 (MQ=255)
                                                                                                ccGCTTCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAg                          >  1:156315/1‑139 (MQ=255)
                                                                                                     tCGTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGaaaa                     >  2:614187/1‑139 (MQ=255)
                                                                                                       gTTGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAgg                   <  1:614187/139‑1 (MQ=255)
                                                                                                        ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                         >  2:35964/1‑36 (MQ=255)
                                                                                                        ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGaa                                                                                                                         <  1:35964/36‑1 (MQ=255)
                                                                                                        ttGTCTGGACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTCCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGa                  <  1:892647/139‑1 (MQ=255)
                                                                                                              ggACAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCaaaa            <  2:1075129/139‑1 (MQ=255)
                                                                                                                aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                    <  2:346254/97‑1 (MQ=255)
                                                                                                                aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCg                                                    >  1:346254/1‑97 (MQ=255)
                                                                                                                aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:142650/114‑1 (MQ=255)
                                                                                                                aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   >  1:142650/1‑114 (MQ=255)
                                                                                                                aCAATCGTTCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAata          <  2:209422/139‑1 (MQ=255)
                                                                                                                        tCCTTTGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAg  <  2:156315/139‑1 (MQ=255)
                                                                                                                             tGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   <  2:1256821/101‑1 (MQ=255)
                                                                                                                             tGTAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGATGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   >  1:1256821/1‑101 (MQ=255)
                                                                                                                               tAACAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTAcc                                   >  2:259801/1‑99 (MQ=255)
                                                                                                                                  |                                                                                                                                
ATCGCTTCGGATAACTTCCTGTTATCCGAAACATATCACTCACTTTGGTGATTTCACCGTAACTGTCTATGATTAATGAGGCGGTGGTTAACACTACCGCTTCGTTGTCTGGACAATCGTTCCTTTGTAATAGGTCCGAATCACAATGGAAGGTTCAAGAATGAAATACCGCATCGCTTTAGCTGTTTCTCTCTTTGCTCTTAGTGCCGGTAGTTATGCCACTACCCTGTGTCAGGAAAAGGAGCAAAATATCCTTAAG  >  NC_000913/3248809‑3249067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑