Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	296.6 / NA	91	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/44);  total (47/44)

GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCA  >  NC_000913/3366825‑3367084                                                                                                                                 |                                                                                                                                    gTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCtt                                                                                                                           >  1:516213/1‑139 (MQ=255)  ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                          <  1:393892/139‑1 (MQ=255)  ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                          <  1:131532/139‑1 (MQ=255)       cTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                     <  2:67410/139‑1 (MQ=255)          acacTGACAATGGCTCCAGCTTTTACGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCAt                                                                                                                  >  2:957056/1‑139 (MQ=255)                aCAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgc                                                                                                            >  1:1115332/1‑139 (MQ=255)                aCAATGGATCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgc                                                                                                            >  2:835097/1‑139 (MQ=255)                 cAATGGCTCCAGCTTGTAAGGACGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATAAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcg                                                                                                           >  1:913836/1‑139 (MQ=255)                    tGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                        <  1:39273/139‑1 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  2:346286/1‑139 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  2:656902/139‑1 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  1:346286/139‑1 (MQ=255)                       cTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGcc                                                                                                     >  2:463903/1‑139 (MQ=255)                          cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGTAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                          >  2:342512/1‑131 (MQ=255)                          cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                          <  1:342512/131‑1 (MQ=255)                          cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                  >  2:1017876/1‑139 (MQ=255)                             cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGGGCAAGCCGCTGAt                                                                                               <  1:1149800/139‑1 (MQ=255)                              ttttAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                              <  2:1115332/139‑1 (MQ=255)                                  aaGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGt                                                                                          >  1:1173347/1‑139 (MQ=255)                                  aaGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGt                                                                                          <  1:33308/139‑1 (MQ=255)                                        gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                    <  2:913836/139‑1 (MQ=255)                                                  tAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAg                                                                          <  2:516213/139‑1 (MQ=255)                                                        aCATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGAct                                                                    <  2:1356395/139‑1 (MQ=255)                                                                   atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGc                                                         <  1:398074/139‑1 (MQ=255)                                                                      aCAGGCAATCTGATGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGc                                                      >  2:1149604/1‑139 (MQ=255)                                                                      aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGGCGTTAAGGTGACTCTGGAAGCTGCAGc                                                      >  2:278817/1‑139 (MQ=255)                                                                      aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGc                                                      >  2:1236106/1‑139 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  1:344782/90‑1 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  2:344782/1‑90 (MQ=255)                                                                        aGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCtt                                                    >  1:914930/1‑139 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTCAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATCGAGTAACAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                   <  2:914930/139‑1 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                          <  2:1192540/84‑1 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAgcgc                                                                                                          >  1:1192540/1‑84 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                <  1:943332/94‑1 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                >  2:943332/1‑94 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                             <  2:1184706/95‑1 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                             >  1:1184706/1‑95 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                 >  1:138673/1‑139 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTg                                                                                                                               <  1:1110450/60‑1 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTg                                                                                                                               >  2:1110450/1‑60 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                          >  2:242149/1‑65 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                          <  1:242149/65‑1 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTAc                                                >  2:1132081/1‑139 (MQ=255)                                                                            aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTAc                                                >  1:700428/1‑139 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTGCTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                              <  1:549570/139‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTGCTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                              >  2:549570/1‑139 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                              >  2:1047547/1‑139 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGATGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                              <  1:957056/139‑1 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  1:25260/1‑73 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  2:25260/73‑1 (MQ=255)                                                                                              gAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcgg                              <  2:138673/139‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCt                                                                                                                            >  2:604435/1‑43 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCt                                                                                                                            <  1:604435/43‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  1:18934/1‑65 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  2:18934/65‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  1:1004068/65‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  2:1004068/1‑65 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    <  2:937918/67‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    >  2:1069684/1‑67 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    <  1:1069684/67‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    >  1:937918/1‑67 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                   >  1:325696/1‑116 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                   <  2:325696/116‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                            >  2:855956/1‑139 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                            >  2:484057/1‑139 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                            <  1:1236106/139‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTATTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTCCTCCAATACTAAGCAAcggcg                            >  1:967034/1‑139 (MQ=255)                                                                                                 gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                    <  1:325767/82‑1 (MQ=255)                                                                                                 gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                    >  2:325767/1‑82 (MQ=255)                                                                                                        gAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAgttgtt                    <  1:855956/139‑1 (MQ=255)                                                                                                           cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  1:1028699/54‑1 (MQ=255)                                                                                                           cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  2:1028699/1‑54 (MQ=255)                                                                                                              tGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  2:268762/51‑1 (MQ=255)                                                                                                              tGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  1:268762/1‑51 (MQ=255)                                                                                                                tAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCgg            <  1:1161978/139‑1 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTTCCTTTTTTGCCATGt                                                                                                                >  2:12098/1‑38 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGt                                                                                                                <  1:12098/38‑1 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcgg                              <  2:621697/120‑1 (MQ=255)                                                                                                                 aaaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcgg                              >  1:621697/1‑120 (MQ=255)                                                                                                                      tACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    <  1:1192285/45‑1 (MQ=255)                                                                                                                      tACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                    >  2:1192285/1‑45 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      <  2:979972/41‑1 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                      >  1:979972/1‑41 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                <  2:426150/47‑1 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                >  1:426150/1‑47 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt    >  2:10536/1‑139 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt    >  1:384207/1‑139 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt    >  1:1066724/1‑139 (MQ=255)                                                                                                                         tGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc   <  2:1066724/139‑1 (MQ=255)                                                                                                                         tGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc   <  1:1017876/139‑1 (MQ=255)                                                                                                                          gATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCa  >  2:948316/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                    GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCA  >  NC_000913/3366825‑3367084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑