Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,434,577	G→A	A122T (GCA→ACA)	fmt →	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,434,577	0	G	A	100.0%	144.5 / NA	45	A122T (GCA→ACA)	fmt	10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

TGGTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCA  >  NC_000913/3434443‑3434706                                                                                                                                       |                                                                                                                                  tGGTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACAcc                                                                                                                               >  1:1271502/1‑139 (MQ=255)       ccGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCa                                                                                                                         <  2:1271502/139‑1 (MQ=255)         gAACTACAGGGTGATGTGACGGTCTCCGTCGCGTATAGGTTACATCTGCCGAAACCAGTGCTGGAGATGCCTGGTCATGGCTTTGTAAATGTTCAAGGTTCCGTCCTGCCGCGCTGGTGATGTCTTACACCAATTCAAc                                                                                                                       <  1:883561/139‑1 (MQ=255)            cTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCt                                                                                                                    <  1:1178410/139‑1 (MQ=255)                          tATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGt                                                                                                      <  1:604510/139‑1 (MQ=255)                                 gtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCGCTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACt                                                                                                                <  1:970656/122‑1 (MQ=255)                                 gtcgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACt                                                                                                                >  2:970656/1‑122 (MQ=255)                                   cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGaa                                                                                             >  2:1162865/1‑139 (MQ=255)                                   cgtcgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGaa                                                                                             >  2:170615/1‑139 (MQ=255)                                      cgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACt                                                                                          >  1:467205/1‑139 (MQ=255)                                      cgCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACt                                                                                          >  1:1262960/1‑139 (MQ=255)                                           aTGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATTGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGgtgt                                                                                     <  2:350902/139‑1 (MQ=255)                                             ggTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGa                                                                                   >  1:1122075/1‑139 (MQ=255)                                              gTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGAc                                                                                  <  1:170615/139‑1 (MQ=255)                                                         ctttcggCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                          >  1:357506/7‑88 (MQ=255)                                                         ctttcggCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                          <  2:357506/82‑1 (MQ=255)                                                         ccGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                               >  2:872020/1‑99 (MQ=255)                                                         ccGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTa                                                                                                               <  1:872020/99‑1 (MQ=255)                                                           gAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATg                                                                     <  1:1221130/139‑1 (MQ=255)                                                                cAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATCCAAATgtacgt                                                                <  1:793721/139‑6 (MQ=255)                                                                 aGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTc                                                               >  2:1179210/1‑139 (MQ=255)                                                                   tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                          >  1:770381/1‑78 (MQ=255)                                                                   tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATcc                                                                                                                          <  2:770381/78‑1 (MQ=255)                                                                   tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaaa                                                                       >  2:1037039/1‑129 (MQ=255)                                                                   tGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCaaa                                                                       <  1:1037039/129‑1 (MQ=255)                                                                         agaTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                       <  2:1122075/139‑1 (MQ=255)                                                                         agaTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                       <  2:1262960/139‑1 (MQ=255)                                                                         agaTGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGa                                                       <  2:467205/139‑1 (MQ=255)                                                                               cgcgTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCgg                                                 >  1:163510/1‑139 (MQ=255)                                                                                  gTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGa                                              >  2:443379/1‑139 (MQ=255)                                                                                                  cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGc                                         <  1:635434/128‑1 (MQ=255)                                                                                                  cGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGc                                         >  2:635434/1‑128 (MQ=255)                                                                                                      cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGAt                                                                  >  2:795398/1‑99 (MQ=255)                                                                                                      cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                 >  1:1052841/1‑100 (MQ=255)                                                                                                      cATGGTTCACTGCTGCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATg                                                                 <  2:1052841/100‑1 (MQ=255)                                                                                                      cATGGTTCACTGCTGCCACGCTGGCGCGGAGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGTAt                                                                  <  1:795398/99‑1 (MQ=255)                                                                                                               ctgctgCCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTa                 <  2:163510/139‑1 (MQ=255)                                                                                                                 gctgcCACGCTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACt               <  2:1307032/139‑1 (MQ=255)                                                                                                                          cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                           >  1:759215/1‑102 (MQ=255)                                                                                                                          cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGatat                                           <  2:759215/102‑1 (MQ=255)                                                                                                                          cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAt                   >  1:1039248/1‑126 (MQ=255)                                                                                                                          cTGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGAt                   <  2:1039248/126‑1 (MQ=255)                                                                                                                           tGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTa                                                         >  2:611805/1‑87 (MQ=255)                                                                                                                           tGGCGCGGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTa                                                         <  1:611805/87‑1 (MQ=255)                                                                                                                              cgcgGTGCTACACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCa  <  1:443379/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  TGGTCGCCGAACTGCAGGCTGATGTTATGGTCGTCGTCGCCTATGGTTTAATTCTGCCGAAAGCAGTGCTGGAGATGCCGCGTCTTGGCTGTATCAACGTTCATGGTTCACTGCTGCCACGCTGGCGCGGTGCTGCACCAATCCAACGCTCACTATGGGCGGGTGATGCAGAAACTGGTGTGACCATTATGCAAATGGATGTCGGTTTAGACACCGGTGATATGCTCTATAAGCTCTCCTGCCCGATTACTGCAGAAGATACCA  >  NC_000913/3434443‑3434706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑