Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,141	C→T	*123* (TAG→TAA)	yhdN ←	DUF1992 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,141	0	C	T	100.0%	146.2 / NA	46	*123* (TAG→TAA)	yhdN	DUF1992 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/20);  total (26/20)

GGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439016‑3439276                                                                                                                              |                                                                                                                                        ggCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGa                                                                                                                            <  1:1128320/139‑1 (MQ=255)     aaaCCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGAtttt                                                                                                                        >  1:642630/1‑139 (MQ=255)       aCCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGt                                                                                                                      <  2:642630/139‑1 (MQ=255)                   tACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGt                                                                                                          <  2:30071/139‑1 (MQ=255)                                    atcatcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACA‑ACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                             <  2:817660/134‑1 (MQ=255)                                    atcatcTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACA‑ACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgcc                                                                                             >  1:817660/1‑134 (MQ=255)                                         ctgctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaaaaa                                                                                    >  2:895962/1‑139 (MQ=255)                                         ctgctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTaaaaa                                                                                    <  1:1305692/139‑1 (MQ=255)                                           gctgTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAAt                                                                                  <  2:372414/139‑1 (MQ=255)                                               tttttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGt                                                                                                                                     >  1:76118/1‑84 (MQ=255)                                               tttttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGt                                                                                                                                     <  2:76118/84‑1 (MQ=255)                                                ttttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTg                                                                                                                       >  1:1251299/1‑97 (MQ=255)                                                ttttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTg                                                                                                                       <  2:1251299/97‑1 (MQ=255)                                                 tttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                                <  2:660418/135‑1 (MQ=255)                                                 tttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCg                                                                                >  1:660418/1‑135 (MQ=255)                                                 tttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTAc                                                                            >  1:1327047/1‑139 (MQ=255)                                                 tttCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTAc                                                                            >  2:899113/1‑139 (MQ=255)                                                              aaTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTg                                                               >  2:798124/1‑139 (MQ=255)                                                               aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTa                                                                             >  1:1032187/1‑124 (MQ=255)                                                               aTCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTa                                                                             <  2:1032187/124‑1 (MQ=255)                                                                   tgtCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATCAGTTATCCTTGTTTTTGTCCAACAACTTGTCAGCATAATCGCCGCGCAAAAAATCGGTACTCAATCCAACTTGTCGcc                                                          >  1:127440/1‑138 (MQ=255)                                                                        ggTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            <  2:452412/84‑1 (MQ=255)                                                                        ggTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACtt                                                                                                            >  1:452412/1‑84 (MQ=255)                                                                        ggTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTc                                                     <  1:895962/139‑1 (MQ=255)                                                                            ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAAtt                                                 >  2:1261312/1‑139 (MQ=255)                                                                            ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGAT‑CATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTc                                                >  1:1070263/1‑139 (MQ=255)                                                                            ttACTTCCGCCATTTTTGCCAGCTCACCAATGCGAT‑CATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTc                                                >  1:438565/1‑139 (MQ=255)                                                                              aCTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgc                                                                                              >  1:596491/1‑92 (MQ=255)                                                                              aCTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATcgc                                                                                              <  2:596491/92‑1 (MQ=255)                                                                                    gCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAAt                                         >  2:491108/1‑139 (MQ=255)                                                                                      cATTTTTGCCAGCTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGa                                       >  1:1364037/1‑139 (MQ=255)                                                                                                 gCTCACCAATGCGAT‑CATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  2:438565/139‑1 (MQ=255)                                                                                                 gCTCACCAATGCGAT‑CATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCt                           <  2:1070263/139‑1 (MQ=255)                                                                                                  cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGt                                                                                                          >  1:646910/1‑60 (MQ=255)                                                                                                  cTCACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGt                                                                                                          <  2:646910/60‑1 (MQ=255)                                                                                                    cACCAATGCGATACATACATACTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTaa                         <  1:491108/139‑1 (MQ=255)                                                                                                               tacatacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCATAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGatat              >  1:414585/1‑139 (MQ=255)                                                                                                                 catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAGAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTg            >  2:823516/1‑139 (MQ=255)                                                                                                                 catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCtt                   <  2:1260958/132‑1 (MQ=255)                                                                                                                 catacatacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCtt                   >  1:1260958/1‑132 (MQ=255)                                                                                                                      atacTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGat       >  1:1069924/1‑139 (MQ=255)                                                                                                                        acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatca     >  2:1294692/1‑139 (MQ=255)                                                                                                                        acTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCATAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGatca     <  2:414585/139‑1 (MQ=255)                                                                                                                         cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCaa                                                   >  1:542167/1‑92 (MQ=255)                                                                                                                         cTCCATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCaa                                                   <  2:542167/92‑1 (MQ=255)                                                                                                                           ccATTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCg  >  1:56322/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        GGCGAAACCCACCTTCAGTACGCACTTCATGCTCCATCATCTGCTGTTTTTCGTAATAACGAATCGTGTCGGGTGTTACTTCCGCCATTTTTGCCAGCTCACCAATGCGATACATACATACTCCACTAGTTATCGTTGATTTTGTCCAACAACTTGTCAGCATAATCGCCGCGTAAAAAATCGGTACTCAATCCAGCTTGTCGCAGCTTCAATTCCAGTAATGACAATCGACGGCTAACCTCTTGATATTGCGGATCATCG  >  NC_000913/3439016‑3439276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑