Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 197.7 / NA 62P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/32);  total (30/32)

ATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCG  >  NC_000913/323221‑323488
                                                                                                                                      |                                                                                                                                     
aTAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAAt                                                                                                                                   >  1:1173863/1‑139 (MQ=255)
 tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAAtt                                                                                                                                  >  2:127270/1‑139 (MQ=255)
       gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGAATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAGTTGGTGCg                                                                                                                            >  2:670986/1‑139 (MQ=255)
                 gTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                       <  1:345274/134‑1 (MQ=255)
                 gTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGAt                                                                                                                       >  2:345274/1‑134 (MQ=255)
                   gCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCg                                                                                                                <  1:670986/139‑1 (MQ=255)
                    cTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGc                                                                                                               >  2:486070/1‑139 (MQ=255)
                            tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                       <  2:515542/139‑1 (MQ=255)
                            tGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                       >  1:763316/1‑139 (MQ=255)
                             gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggcg                                                                                                      <  1:1338477/139‑1 (MQ=255)
                             gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggcg                                                                                                      <  2:489080/139‑1 (MQ=255)
                                      gATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                                                             >  1:1093662/1‑139 (MQ=255)
                                            ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatttta                                                                                       <  2:1173863/139‑1 (MQ=255)
                                                  tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                                                 <  1:486070/139‑1 (MQ=255)
                                                         gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTa                                                                          <  2:545533/139‑1 (MQ=255)
                                                             cGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                                                      <  1:127270/139‑1 (MQ=255)
                                                              ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                     >  2:317599/1‑139 (MQ=255)
                                                                ggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATCGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   >  1:1018679/1‑139 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                               >  1:1192814/1‑63 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                               <  2:1192814/63‑1 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                         >  2:1244215/1‑133 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                         <  1:1244215/133‑1 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                   >  1:467325/1‑139 (MQ=255)
                                                                                tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                   >  2:509045/1‑139 (MQ=255)
                                                                                 gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtct                                                                                                                   <  2:93917/74‑1 (MQ=255)
                                                                                 gTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtct                                                                                                                   >  1:93917/1‑74 (MQ=255)
                                                                                      gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  1:1104004/80‑1 (MQ=255)
                                                                                      gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  2:1104004/1‑80 (MQ=255)
                                                                                             gCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtg                                      <  1:509045/139‑1 (MQ=255)
                                                                                                atatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                                   <  1:423821/139‑1 (MQ=255)
                                                                                                atatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                                   <  2:763316/139‑1 (MQ=255)
                                                                                                   ttGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt                                <  2:467325/139‑1 (MQ=255)
                                                                                                   ttGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt                                <  2:605886/139‑1 (MQ=255)
                                                                                                     ggCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGc                              >  1:442486/1‑139 (MQ=255)
                                                                                                        ggTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTa                           >  1:254535/1‑139 (MQ=255)
                                                                                                         gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  2:741209/61‑1 (MQ=255)
                                                                                                         gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  1:741209/1‑61 (MQ=255)
                                                                                                         gTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTAt                          >  1:1292761/1‑139 (MQ=255)
                                                                                                                atatGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                                                       >  2:30043/1‑103 (MQ=255)
                                                                                                                atatGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                                                       <  1:30043/103‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  2:1301865/1‑50 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  1:1301865/50‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   <  2:772051/87‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   >  1:427112/1‑87 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   >  1:772051/1‑87 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                   <  2:427112/87‑1 (MQ=255)
                                                                                                                    ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa               >  2:666329/1‑139 (MQ=255)
                                                                                                                        ctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        <  2:1292917/46‑1 (MQ=255)
                                                                                                                        ctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                                                        >  1:1292917/1‑46 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                            <  1:777522/73‑1 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                            >  2:777522/1‑73 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGc                     >  2:318628/1‑128 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGc                     <  1:318628/128‑1 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTg          >  1:137765/1‑139 (MQ=255)
                                                                                                                         tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTg          >  1:1129192/1‑139 (MQ=255)
                                                                                                                            ttATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGAtt       <  1:739105/139‑1 (MQ=255)
                                                                                                                              aTCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGAtttt     <  2:1129192/139‑1 (MQ=255)
                                                                                                                                ccAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                                                       <  2:602468/71‑1 (MQ=255)
                                                                                                                                ccAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                                                       >  1:602468/1‑71 (MQ=255)
                                                                                                                                 cAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGTCAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCg  <  1:317599/139‑1 (MQ=255)
                                                                                                                                 cAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCg  <  1:666329/139‑1 (MQ=255)
                                                                                                                                 cAGGGTCAATCGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCg  <  2:1018679/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
ATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCG  >  NC_000913/323221‑323488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑