Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,970	C→T	Q16* (CAA→TAA)	yahE →	DUF2877 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,970	0	C	T	100.0%	168.4 / NA	52	Q16* (CAA→TAA)	yahE	DUF2877 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/26);  total (26/26)

GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCA  >  NC_000913/335834‑336097                                                                                                                                         |                                                                                                                                ggAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGAGTGTATGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTaa                                                                                                                               >  1:34503/1‑139 (MQ=255)   aaCGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                             <  2:510830/139‑1 (MQ=255)    aCGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGa                                                                                                                            <  2:34503/139‑1 (MQ=255)      ggggCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACa                                                                                                                          <  2:1189694/139‑1 (MQ=255)      ggggCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACa                                                                                                                          <  1:376651/139‑1 (MQ=255)              gAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGTCGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAAc                                                                                                                  <  1:223633/139‑1 (MQ=255)                   gaTTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTc                                                                                                             >  2:611800/1‑139 (MQ=255)                       gcgcAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTg                                                                                                         >  2:453682/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGa                                                                                                                            >  1:147706/1‑104 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGa                                                                                                                            <  2:147706/104‑1 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                         >  2:465578/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                         >  2:725506/1‑139 (MQ=255)                                       ccGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAatat                                                                                         >  2:733434/1‑139 (MQ=255)                                          cAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGc                                                                                      >  1:755354/1‑139 (MQ=255)                                                     aCCGGGAGGCTTGCTATCAACACACCAGATAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTc                                                                           >  2:913053/1‑139 (MQ=255)                                                     aCCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGATCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTc                                                                           >  2:859615/1‑139 (MQ=255)                                                          gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACt                                                                                   <  2:58080/126‑1 (MQ=255)                                                          gAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACt                                                                                   >  1:58080/1‑126 (MQ=255)                                                                      cAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACg                                                          <  2:187057/139‑1 (MQ=255)                                                                       aaCACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgc                                                         >  1:1114777/1‑139 (MQ=255)                                                                        acacacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                        >  2:777019/1‑139 (MQ=255)                                                                        acacacCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACgct                                                        <  1:298595/139‑1 (MQ=255)                                                                             ccAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCttt                                                   >  2:348353/1‑139 (MQ=255)                                                                                 aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGt                                                                                                              >  1:214597/1‑76 (MQ=255)                                                                                 aaaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGt                                                                                                              <  2:214597/76‑1 (MQ=255)                                                                                  aaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                              <  2:755354/139‑1 (MQ=255)                                                                                  aaGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                              <  2:246424/139‑1 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                    >  2:979581/1‑81 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                 <  2:260821/84‑1 (MQ=255)                                                                                      cGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCg                                                                                                 >  1:260821/1‑84 (MQ=255)                                                                                      cGGTATGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCaga                                                                                                    <  1:979581/81‑1 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                              <  1:717748/133‑1 (MQ=255)                                                                                        gtgtgtgtGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGaa                                              >  2:717748/1‑133 (MQ=255)                                                                                           tgtgtgGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGAc                                     >  1:323841/1‑139 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                      <  1:595113/66‑1 (MQ=255)                                                                                                   cgcTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCa                                                                                                      >  2:595113/1‑66 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                             <  2:1094977/41‑1 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAgg                                                                                                                             >  1:1094977/1‑41 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGa                                                            >  2:245765/1‑106 (MQ=255)                                                                                                     cTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGa                                                            <  1:245765/106‑1 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                              <  1:1090644/86‑1 (MQ=255)                                                                                                       aaCTGCGGATGCGGATTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCgg                                                                              >  2:1090644/1‑86 (MQ=255)                                                                                                           gcggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGtt                     >  2:678805/1‑139 (MQ=255)                                                                                                            cggatgcggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTg                    <  2:382753/139‑1 (MQ=255)                                                                                                                  cggatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGtt              >  2:71024/1‑139 (MQ=255)                                                                                                                    gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAg                                                                                                                >  1:995960/1‑39 (MQ=255)                                                                                                                    gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAg                                                                                                                <  2:995960/39‑1 (MQ=255)                                                                                                                    gatTTTCTGGCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTgg            <  1:725506/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact    <  2:323841/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact    <  2:859145/139‑1 (MQ=255)                                                                                                                            ggCGCAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcact    <  1:678805/139‑1 (MQ=255)                                                                                                                              cgcAGCGGGGGTAAGGACAGGTTGAACAGGTCTTTGACAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGcactcactca  <  2:857782/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGTGCATAAACCGGGAGGCTTGCTATCAACACACCAGAAAGACGGTGTGTGTGGGCGCTAACTGCGGATGCGGATTTTCTGGCGCAGCGGGGGCAAGGACAGGTTGAACAGGTCTTTGCCAGAGCGGTAAATATCGCACTCCCGGCTCGCCAGCAGTTGCTGACGCTGCTTTGTGAAGAGTACGACAATGCGCCAAACAGTTGTCGGTTGGCACTCACTCA  >  NC_000913/335834‑336097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑