Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,736,157 G→A *393* (TAG→TAA xylR → xylose divergent operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,1570GA100.0% 220.3 / NA 68*393* (TAG→TAAxylRxylose divergent operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/31);  total (37/31)

CAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCG  >  NC_000913/3736037‑3736279
                                                                                                                        |                                                                                                                           
cAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTc                                                                                                           >  2:283683/1‑139 (MQ=255)
           cGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATc                                                                                                >  2:1191197/1‑139 (MQ=255)
                aTGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTa                                                                                           >  2:1265784/1‑139 (MQ=255)
                  gtgCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAAc                                                                                         >  2:1058465/1‑139 (MQ=255)
                      ggTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTg                                                                                     <  1:501131/139‑1 (MQ=255)
                          aTCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCgg                                                                                                         >  1:1060800/1‑115 (MQ=255)
                          aTCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCgg                                                                                                         <  2:1060800/115‑1 (MQ=255)
                          aTCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTg                                                                                 <  1:447249/139‑1 (MQ=255)
                           tCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGCGAATTGTc                                                                                                           >  2:825178/1‑112 (MQ=255)
                           tCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTc                                                                                                           <  1:825178/112‑1 (MQ=255)
                                cGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTc                                                                           >  1:1130172/1‑139 (MQ=255)
                                cGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTc                                                                           >  1:152060/1‑139 (MQ=255)
                                  cTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCgg                                                                         >  1:379122/1‑139 (MQ=255)
                                            tCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTg                                                               <  2:379122/139‑1 (MQ=255)
                                             cTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGa                                                              >  1:70630/1‑139 (MQ=255)
                                               aCTCTGTTTTTAAACAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTCACGAATGATGAGAATTGTCGGCGTCAGATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAAc                                                            >  2:831166/1‑139 (MQ=255)
                                                  ctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGATAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGTGTGAACGcc                                                         >  1:807073/1‑139 (MQ=255)
                                                  ctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCTGGTAACGCTGCg                                                                                   <  2:1335724/113‑1 (MQ=255)
                                                  ctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg                                                                                   >  1:1335724/1‑113 (MQ=255)
                                                   tGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg                                                                                   <  1:512889/112‑1 (MQ=255)
                                                   tGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg                                                                                   >  2:512889/1‑112 (MQ=255)
                                                     tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGc                                                                                                        >  2:1355439/1‑89 (MQ=255)
                                                     tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGc                                                                                                        <  1:1355439/89‑1 (MQ=255)
                                                     tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTcaca                                                                                                  <  1:488242/95‑1 (MQ=255)
                                                     tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTcaca                                                                                                  >  2:488242/1‑95 (MQ=255)
                                                     tttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTa                                                      >  1:806007/1‑139 (MQ=255)
                                                       tttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATc                                                    <  1:824752/139‑1 (MQ=255)
                                                          aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg                                                                                                                           >  2:612233/1‑65 (MQ=255)
                                                          aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACg                                                                                                                           <  1:612233/65‑1 (MQ=255)
                                                               aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTaa                                                                                          >  1:362973/1‑93 (MQ=255)
                                                               aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTaa                                                                                          <  2:362973/93‑1 (MQ=255)
                                                                       acacGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAgg                                    <  2:806007/139‑1 (MQ=255)
                                                                       acacGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAgg                                    <  2:152060/139‑1 (MQ=255)
                                                                        cacGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGc                                   <  2:955117/139‑1 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACgaatg                                                                                                                      <  2:457171/52‑4 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACgaatg                                                                                                                      >  1:457171/1‑49 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg                                                                                   >  2:554996/1‑88 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCg                                                                                   <  1:554996/88‑1 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCg                                                  >  1:1285188/1‑121 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCg                                                  <  2:1285188/121‑1 (MQ=255)
                                                                           gacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                >  1:120552/1‑139 (MQ=255)
                                                                              gCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATa                             >  1:605555/1‑139 (MQ=255)
                                                                                 aaaaGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaaaa                          <  1:283683/139‑1 (MQ=255)
                                                                                   aaGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCt                                              >  2:59088/1‑117 (MQ=255)
                                                                                   aaGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCt                                              <  1:59088/117‑1 (MQ=255)
                                                                                    agagTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGc                       <  1:831166/139‑1 (MQ=255)
                                                                                    agagTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGc                       <  1:410248/139‑1 (MQ=255)
                                                                                     gagTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTg                                                               <  1:353237/98‑1 (MQ=255)
                                                                                     gagTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTg                                                               >  2:353237/1‑98 (MQ=255)
                                                                                       gTATCGCGATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAcc                    >  1:982631/1‑139 (MQ=255)
                                                                                            gcgATTTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaacaa               <  2:1152229/139‑1 (MQ=255)
                                                                                             cgATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCaacaac              >  1:666886/1‑139 (MQ=255)
                                                                                             cgATGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCaacaac              >  2:510273/1‑139 (MQ=255)
                                                                                                tGTAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCt           >  1:506247/1‑139 (MQ=255)
                                                                                                  tAAATAGCGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTc         >  1:898400/1‑139 (MQ=255)
                                                                                                         cGAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCg  <  2:898400/139‑1 (MQ=255)
                                                                                                          gAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaaca                >  1:1298103/1‑124 (MQ=255)
                                                                                                          gAGGTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaaca                <  2:1298103/124‑1 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg                                                                    <  2:1276549/70‑1 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATgcg                                                                    >  1:1276549/1‑70 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                             <  1:164982/93‑1 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                             >  2:164982/1‑93 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAc                     >  2:550852/1‑117 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAc                     <  1:550852/117‑1 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaac                 <  2:558136/121‑1 (MQ=255)
                                                                                                            ggTCATGTTGTAACGAATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaac                 >  1:558136/1‑121 (MQ=255)
                                                                                                                  gttgtAACGAATGATGAGAATTGTCGGCGTCACATCAGGTaa                                                                                          <  2:761969/42‑1 (MQ=255)
                                                                                                                  gttgtAACGAATGATGAGAATTGTCGGCGTCACATCAGGTaa                                                                                          >  1:761969/1‑42 (MQ=255)
                                                                                                                        |                                                                                                                           
CAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCG  >  NC_000913/3736037‑3736279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: