Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,792,826	C→T	*286* (TAG→TAA)	yibB ←	YibB family protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,792,826	0	C	T	100.0%	178.5 / NA	55	*286* (TAG→TAA)	yibB	YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)

TACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATA  >  NC_000913/3792699‑3792960                                                                                                                                |                                                                                                                                       tACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGa                                                                                                                             <  2:33753/139‑1 (MQ=255)        ttGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGaaa                                                                                                                      >  1:896466/1‑139 (MQ=255)           gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAAtt                                                                                                                   >  2:430495/1‑139 (MQ=255)                gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTa                                                                                                                                      >  2:1280908/1‑115 (MQ=255)                gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTa                                                                                                                                      <  1:1280908/115‑1 (MQ=255)                            ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                  >  2:281345/1‑139 (MQ=255)                             tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttttt                                                                                                 <  2:455053/139‑1 (MQ=255)                                  ttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                              >  1:1059418/1‑121 (MQ=255)                                  ttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                              <  2:1059418/121‑1 (MQ=255)                                         aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATTGAGGGAAACTTATTTTCTTGAAAGGACAATTCACAGCGCCTGCATTTTTGCCCCTAAAgtg                                                                                     <  1:883926/139‑1 (MQ=255)                                         aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                                                                     <  1:430495/139‑1 (MQ=255)                                          ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                    >  2:1084258/1‑139 (MQ=255)                                             atatAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                              <  2:563756/110‑1 (MQ=255)                                             atatAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                              >  1:563756/1‑110 (MQ=255)                                                  aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTg                                                                            >  1:1136078/1‑139 (MQ=255)                                                       aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagca                                                                       >  2:405531/1‑139 (MQ=255)                                                       aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagca                                                                       >  2:142296/1‑139 (MQ=255)                                                                 gCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                         >  2:513540/1‑111 (MQ=255)                                                                 gCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                         <  1:513540/111‑1 (MQ=255)                                                                 gCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACaaa                                                             >  2:937240/1‑139 (MQ=255)                                                                               tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                               <  1:1084258/139‑1 (MQ=255)                                                                                ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                              <  1:937240/139‑1 (MQ=255)                                                                                 tCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                             <  2:1195579/139‑1 (MQ=255)                                                                                     aTCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGcc                                                                                                          <  1:292922/74‑1 (MQ=255)                                                                                     aTCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGcc                                                                                                          >  2:292922/1‑74 (MQ=255)                                                                                      tctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTcccc                                        >  2:902747/1‑139 (MQ=255)                                                                                        tcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                      <  1:902747/139‑1 (MQ=255)                                                                                          aGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                      >  1:87232/1‑137 (MQ=255)                                                                                          aGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                      <  2:87232/137‑1 (MQ=255)                                                                                             tGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                 <  1:142296/139‑1 (MQ=255)                                                                                             tGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                 <  1:281345/139‑1 (MQ=255)                                                                                                tATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGa                                                                                                                             <  1:469961/44‑1 (MQ=255)                                                                                                tATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGa                                                                                                                             >  2:469961/1‑44 (MQ=255)                                                                                                tATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTa                              >  1:872712/1‑139 (MQ=255)                                                                                                 aTCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                    >  1:479817/1‑84 (MQ=255)                                                                                                 aTCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                    <  2:479817/84‑1 (MQ=255)                                                                                                 aTCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                              >  2:352860/1‑122 (MQ=255)                                                                                                 aTCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                              <  1:352860/122‑1 (MQ=255)                                                                                                      aaCGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGa                        >  1:1062122/1‑139 (MQ=255)                                                                                                         gCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAaga                     >  1:1328184/1‑139 (MQ=255)                                                                                                            aCCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGAt                  >  2:506429/1‑139 (MQ=255)                                                                                                             cccACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATc                 >  1:30654/1‑139 (MQ=255)                                                                                                                aCTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              >  2:703135/1‑139 (MQ=255)                                                                                                                aCTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              <  2:1295680/139‑1 (MQ=255)                                                                                                                aCTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCACTCCCCAGATAGTTTAGGTTGAAGAGATCggg              <  2:30654/139‑1 (MQ=255)                                                                                                                  tAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGc            <  1:703135/139‑1 (MQ=255)                                                                                                                     tGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                         <  1:1053506/59‑1 (MQ=255)                                                                                                                     tGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                         >  2:1053506/1‑59 (MQ=255)                                                                                                                       gAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                              <  2:470548/100‑1 (MQ=255)                                                                                                                       gAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                              >  1:470548/1‑100 (MQ=255)                                                                                                                       gAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              >  2:522035/1‑132 (MQ=255)                                                                                                                       gAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg              <  1:522035/132‑1 (MQ=255)                                                                                                                          ggAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                     <  2:703171/106‑1 (MQ=255)                                                                                                                          ggAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                     >  1:703171/1‑106 (MQ=255)                                                                                                                            aaaCTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATa  >  2:64429/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                       TACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATA  >  NC_000913/3792699‑3792960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑