Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,870,441	G→A	*355* (TAG→TAA)  G430G (GGC→GGT)	cbrA → dgoT ←	colicin M resistance protein, FAD‑binding protein, putative oxidoreductase D‑galactonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,870,441	0	G	A	100.0%	199.3 / NA	61	*355* (TAG→TAA)  G430G (GGC→GGT)	cbrA dgoT	colicin M resistance protein, FAD‑binding protein, putative oxidoreductase D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

CGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAA  >  NC_000913/3870315‑3870568                                                                                                                               |                                                                                                                                cgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTc                                                                                                                     >  2:1271414/1‑139 (MQ=255)  gcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                    <  2:714465/139‑1 (MQ=255)          cAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                            >  2:1158687/1‑139 (MQ=255)          cAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                            >  2:723166/1‑139 (MQ=255)               tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       <  1:723166/139‑1 (MQ=255)                 cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:1158687/139‑1 (MQ=255)                 cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:779111/139‑1 (MQ=255)                 cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:1188271/139‑1 (MQ=255)                 cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:351098/139‑1 (MQ=255)                 cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:1296666/139‑1 (MQ=255)                   tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                            >  1:752486/1‑114 (MQ=255)                   tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                            <  2:752486/114‑1 (MQ=255)                   tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                   <  1:376654/139‑1 (MQ=255)                         aCTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATaa                                                                                             <  1:307654/139‑1 (MQ=255)                              tCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAagagag                                                                                        >  2:1213142/1‑139 (MQ=255)                              tCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAagagag                                                                                        >  1:713981/1‑139 (MQ=255)                                   aaGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcgc                                                                                   <  1:1213142/139‑1 (MQ=255)                                         gTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCa                                                                             >  1:1157732/1‑139 (MQ=255)                                               aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                   >  1:162978/1‑111 (MQ=255)                                               aGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                   <  2:162978/111‑1 (MQ=255)                                                gCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAc                                                                      >  2:1170584/1‑139 (MQ=255)                                                  cGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                           >  1:1364255/1‑84 (MQ=255)                                                  cGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAcg                                                                                                                           <  2:1364255/84‑1 (MQ=255)                                                    aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                            <  1:1177937/81‑1 (MQ=255)                                                    aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                            >  2:1177937/1‑81 (MQ=255)                                                     tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                 <  1:1170584/139‑1 (MQ=255)                                                     tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCAAATATTCCACAGTTGAAGGATTAACCAACGCGCTTCGCATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                 <  2:51801/139‑1 (MQ=255)                                                          gACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGata                                                            <  2:713981/139‑1 (MQ=255)                                                           aCCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       <  1:450820/95‑1 (MQ=255)                                                           aCCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       >  2:450820/1‑95 (MQ=255)                                                                             aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                         <  2:1157732/139‑1 (MQ=255)                                                                              aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaa                                        >  1:72719/1‑139 (MQ=255)                                                                               gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaaa                                       >  1:1143608/1‑139 (MQ=255)                                                                                       tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     >  2:658853/1‑69 (MQ=255)                                                                                       tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:658853/69‑1 (MQ=255)                                                                                        gcgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                    >  1:48110/1‑53 (MQ=255)                                                                                        gcgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTca                                                                                                                    <  2:48110/53‑1 (MQ=255)                                                                                         cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  2:570302/67‑1 (MQ=255)                                                                                         cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     >  1:570302/1‑67 (MQ=255)                                                                                         cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  1:208825/67‑1 (MQ=255)                                                                                         cgcAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     >  2:208825/1‑67 (MQ=255)                                                                                                   tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                         >  2:681764/1‑117 (MQ=255)                                                                                                   tgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                         <  1:681764/117‑1 (MQ=255)                                                                                                        caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                            <  2:1173740/45‑1 (MQ=255)                                                                                                        caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                            >  1:1173740/1‑45 (MQ=255)                                                                                                        caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       <  2:828013/50‑1 (MQ=255)                                                                                                        caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       >  1:828013/1‑50 (MQ=255)                                                                                                        caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCcca              >  1:962543/1‑139 (MQ=255)                                                                                                            ttCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccaccac          <  1:1271414/139‑1 (MQ=255)                                                                                                               cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAg       >  2:550676/1‑139 (MQ=255)                                                                                                                  aGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCgg    <  2:72719/139‑1 (MQ=255)                                                                                                                   gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcgc                                                                                   <  2:382477/59‑1 (MQ=255)                                                                                                                   gTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcgc                                                                                   >  1:382477/1‑59 (MQ=255)                                                                                                                    ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     >  1:78898/1‑40 (MQ=255)                                                                                                                    ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  2:78898/40‑1 (MQ=255)                                                                                                                    ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       <  2:103741/38‑1 (MQ=255)                                                                                                                    ttGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                       >  1:103741/1‑38 (MQ=255)                                                                                                                    ttGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCGACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGaa  >  2:264122/1‑139 (MQ=255)                                                                                                                     tGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     >  1:806988/1‑39 (MQ=38)                                                                                                                     tGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                     <  2:806988/39‑1 (MQ=38)                                                                                                                            ttAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGata                                                            >  1:383298/1‑73 (MQ=255)                                                                                                                               |                                                                                                                                CGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAA  >  NC_000913/3870315‑3870568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑