Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,609	G→A	*196* (TAG→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,609	0	G	A	100.0%	176.2 / NA	55	*196* (TAG→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC  >  NC_000913/3898479‑3898734                                                                                                                                   |                                                                                                                              aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  1:1124809/139‑1 (MQ=255) aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  1:1299628/139‑1 (MQ=255)    aTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaata                                                                                                                    >  2:44931/1‑139 (MQ=255)      tgtgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                  >  2:782165/1‑139 (MQ=255)      tgtgAGAATTTCGGTATTAGAAATTCTGAACTAGCTAAATGCCTGAAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGCGTGATTTTTCGTAATTATTTAaataat                                                                                                                  >  1:789180/1‑139 (MQ=255)        tgAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATga                                                                                                                >  1:222656/1‑139 (MQ=255)                  ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAGATAATGAGAACAGGCCg                                                                                                      <  1:782165/139‑1 (MQ=255)                  ggTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                      >  1:678119/1‑139 (MQ=255)                                   tCTAGCTAACTGCCTGGAAAAGGGTGGTGATTGTCAGGGTTAGCTCTTCCGCTGTCTCCACTGCGGCAAGCTGGGACGGTGGGGTGATTTTTCGTAATTAGTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACAt                                                                                     <  1:937424/139‑1 (MQ=255)                                     tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                   <  2:222656/139‑1 (MQ=255)                                     tAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                   <  2:678119/139‑1 (MQ=255)                                          aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  2:1134212/127‑1 (MQ=255)                                          aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  1:1134212/1‑127 (MQ=255)                                          aaaTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              <  2:734420/139‑1 (MQ=255)                                            aTGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTa                                                                            >  2:614173/1‑139 (MQ=255)                                                cTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTc                                                                        <  1:475275/139‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  1:415123/120‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  2:415123/1‑120 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       >  2:1206413/1‑139 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       <  1:1208053/139‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                       >  2:440836/1‑139 (MQ=255)                                                           tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                  <  2:1212937/118‑1 (MQ=255)                                                           tggtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCg                                                                                  >  1:1212937/1‑118 (MQ=255)                                                             gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:440836/139‑1 (MQ=255)                                                                            aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                            <  1:51093/59‑1 (MQ=255)                                                                            aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                            >  2:51093/1‑59 (MQ=255)                                                                            aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCg                                            >  1:198586/1‑139 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       >  1:577623/1‑54 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  2:577623/54‑1 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                      <  1:957656/103‑1 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCtt                                                                      >  2:957656/1‑103 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCtt                                  >  2:986423/1‑139 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTTGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                               <  1:986423/139‑1 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGc                                                                                                  <  2:204781/72‑1 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGc                                                                                                  >  1:204781/1‑72 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          >  1:421104/1‑80 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                          <  2:421104/80‑1 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                               <  1:237211/139‑1 (MQ=255)                                                                                           tcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                             >  2:937449/1‑139 (MQ=255)                                                                                            ccACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                            <  1:614173/139‑1 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       <  2:538902/41‑1 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                       >  1:538902/1‑41 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              >  1:317499/1‑67 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCt                                                                              <  2:317499/67‑1 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  2:377178/1‑86 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:377178/86‑1 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGc                                    >  2:1058052/1‑109 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGc                                    <  1:1058052/109‑1 (MQ=255)                                                                                                                   ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  2:808969/85‑1 (MQ=255)                                                                                                                   ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  1:808969/1‑85 (MQ=255)                                                                                                                    gggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           <  1:576886/84‑1 (MQ=255)                                                                                                                    gggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                           >  2:576886/1‑84 (MQ=255)                                                                                                                      gTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc  >  1:683860/1‑139 (MQ=255)                                                                                                                            tttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCg                                      <  1:493475/97‑1 (MQ=255)                                                                                                                            tttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCg                                      >  2:493475/1‑97 (MQ=255)                                                                                                                                   |                                                                                                                              AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC  >  NC_000913/3898479‑3898734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑