Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,939,219 G→A *331* (TAG→TAA) 
S465F (TCT→TTT) 		 rbsR →
hsrA ←		 transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,939,2190GA100.0% 294.6 / NA 89*331* (TAG→TAA) 
S465F (TCT→TTT) 		rbsR
hsrA		transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/45);  total (44/45)

CTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCC  >  NC_000913/3939089‑3939353
                                                                                                                                  |                                                                                                                                      
cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                <  2:435741/139‑1 (MQ=255)
 tATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                               >  1:1167438/1‑139 (MQ=255)
  aTCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTg                                                                                                                              >  2:1019802/1‑139 (MQ=255)
  aTCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTg                                                                                                                              >  1:97666/1‑139 (MQ=255)
       ccaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCttt                                                                                                                         <  2:1171451/139‑1 (MQ=255)
           ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 <  1:1080059/127‑1 (MQ=255)
           ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 >  2:1080059/1‑127 (MQ=255)
           ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                     <  2:554756/139‑1 (MQ=255)
           ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                     <  2:122483/139‑1 (MQ=255)
           ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                     >  1:554756/1‑139 (MQ=255)
             gAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCa                                                                                                                   >  1:869143/1‑139 (MQ=255)
             gAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCa                                                                                                                   >  1:230496/1‑139 (MQ=255)
              aaaGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCaa                                                                                                                  >  2:36295/1‑139 (MQ=255)
                  aTGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAatta                                                                                                              <  2:97666/139‑1 (MQ=255)
                     aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 <  2:180750/117‑1 (MQ=255)
                     aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 >  1:180750/1‑117 (MQ=255)
                     aaCGGGGGGAGCTGGCGATTAATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCATTTGATCAAAttatta                                                                                                           >  2:929084/1‑139 (MQ=255)
                      aCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCt                                                                                                                           >  1:18618/1‑122 (MQ=255)
                      aCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCt                                                                                                                           <  2:18618/122‑1 (MQ=255)
                              aGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                  <  2:869143/139‑1 (MQ=255)
                               gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                     <  2:534191/119‑1 (MQ=255)
                               gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                     >  1:534191/1‑119 (MQ=255)
                               gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttg                                                                                                 >  2:505774/1‑139 (MQ=255)
                                cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgt                                                                                                >  2:142789/1‑139 (MQ=255)
                                     gATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                           <  1:505774/139‑1 (MQ=255)
                                     gATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                           <  1:142789/139‑1 (MQ=255)
                                       ttGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                         <  1:36295/139‑1 (MQ=255)
                                        tGATGTTCTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGaa                                                                                        >  1:973328/1‑139 (MQ=255)
                                         gATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAg                                                                                       <  2:1312385/139‑1 (MQ=255)
                                          aTGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      <  2:230496/139‑1 (MQ=255)
                                          aTGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      <  1:929084/139‑1 (MQ=255)
                                             tACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATg                                                                                   <  1:1019802/139‑1 (MQ=255)
                                                  atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 <  2:552637/88‑1 (MQ=255)
                                                  atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 >  1:552637/1‑88 (MQ=255)
                                                    ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                    <  2:144085/115‑1 (MQ=255)
                                                    ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                    >  1:144085/1‑115 (MQ=255)
                                                     catcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                           >  2:158985/1‑139 (MQ=255)
                                                     catcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                           >  2:288333/1‑139 (MQ=255)
                                                       tcGGATAACCCAGCCGACCCTTCAGCAACAACGGTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                       <  2:1369209/109‑1 (MQ=255)
                                                       tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                       >  1:1369209/1‑109 (MQ=255)
                                                       tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctg                                                                         >  1:452599/1‑139 (MQ=255)
                                                       tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctg                                                                         >  1:94850/1‑139 (MQ=255)
                                                         ggATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTgctgct                                                                       <  2:452599/139‑1 (MQ=255)
                                                         ggATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTgctgct                                                                       <  2:94850/139‑1 (MQ=255)
                                                             aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAg                                                                   <  2:897635/139‑1 (MQ=255)
                                                              aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                  <  1:752197/139‑1 (MQ=255)
                                                               cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCa                                                                 >  2:892846/1‑139 (MQ=255)
                                                                ccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                <  1:459260/139‑1 (MQ=255)
                                                                  aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                >  1:514992/1‑73 (MQ=255)
                                                                  aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                <  2:514992/73‑1 (MQ=255)
                                                                    ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGGACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGt                                                            >  2:133876/1‑139 (MQ=255)
                                                                    ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGt                                                            >  1:1121195/1‑139 (MQ=255)
                                                                    ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGt                                                            >  1:146130/1‑139 (MQ=255)
                                                                       aCCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaat                                                         <  2:1167438/139‑1 (MQ=255)
                                                                           ttCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATg                                                     <  2:20594/139‑1 (MQ=255)
                                                                            tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaat                                                         >  2:825677/1‑134 (MQ=255)
                                                                            tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaat                                                         <  1:825677/134‑1 (MQ=255)
                                                                            tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGc                                                    >  1:686650/1‑139 (MQ=255)
                                                                                     acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                           <  1:288333/139‑1 (MQ=255)
                                                                                      cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 <  1:955202/52‑1 (MQ=255)
                                                                                      cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                                 >  2:955202/1‑52 (MQ=255)
                                                                                      cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                    >  2:688847/1‑81 (MQ=255)
                                                                                      cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                    <  1:688847/81‑1 (MQ=255)
                                                                                        aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaa                                        >  2:1142907/1‑139 (MQ=255)
                                                                                          tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                <  1:219054/49‑1 (MQ=255)
                                                                                          tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                >  2:219054/1‑49 (MQ=255)
                                                                                           aCAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                     <  1:892846/139‑1 (MQ=255)
                                                                                                 tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                               <  1:413627/43‑1 (MQ=255)
                                                                                                 tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                               >  2:413627/1‑43 (MQ=255)
                                                                                                 tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                >  1:1334048/1‑106 (MQ=255)
                                                                                                 tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                <  2:1334048/106‑1 (MQ=255)
                                                                                                      cGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                         <  1:370843/76‑1 (MQ=255)
                                                                                                      cGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                         >  2:370843/1‑76 (MQ=255)
                                                                                                       gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                <  1:1023003/36‑1 (MQ=255)
                                                                                                       gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                                >  2:1023003/1‑36 (MQ=255)
                                                                                                        aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                           <  2:1130024/72‑1 (MQ=255)
                                                                                                        aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                           >  1:1130024/1‑72 (MQ=255)
                                                                                                              aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTaataat                                                      >  1:174598/1‑103 (MQ=255)
                                                                                                              aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTaataat                                                      <  2:174598/103‑1 (MQ=255)
                                                                                                                  aaCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                               <  2:64006/90‑1 (MQ=255)
                                                                                                                  aaCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                               >  1:64006/1‑90 (MQ=255)
                                                                                                                   aCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg             <  2:1121195/139‑1 (MQ=255)
                                                                                                                    cgcgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      >  1:63045/1‑65 (MQ=255)
                                                                                                                    cgcgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      <  2:63045/65‑1 (MQ=255)
                                                                                                                    cgcgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGt            >  1:733526/1‑139 (MQ=255)
                                                                                                                      cgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      <  2:1073764/63‑1 (MQ=255)
                                                                                                                      cgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                      >  1:1073764/1‑63 (MQ=255)
                                                                                                                        gTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg        <  1:1175348/139‑1 (MQ=255)
                                                                                                                              cTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTcc  >  1:657187/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                      
CTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCC  >  NC_000913/3939089‑3939353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑