Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,898	C→T	S582S (AGC→AGT)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,898	0	C	T	100.0%	188.7 / NA	58	S582S (AGC→AGT)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

ACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGG  >  NC_000913/3992767‑3993026                                                                                                                                    |                                                                                                                                 acaccgaAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGCATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           >  1:599079/1‑139 (MQ=255) acaccgaAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           >  2:738799/1‑139 (MQ=255)   accgaAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCgag                                                                                                                         >  2:859669/1‑139 (MQ=255)    ccgaAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCgaga                                                                                                                        <  2:1149483/139‑1 (MQ=255)       aaGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATc                                                                                                                     >  2:747094/1‑139 (MQ=255)             ctctACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAAtt                                                                                                               <  1:859669/139‑1 (MQ=255)               ctACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGc                                                                                                             <  1:738799/139‑1 (MQ=255)                tACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGcc                                                                                                            <  2:1318175/139‑1 (MQ=255)                     cccGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTggta                                                                                                       >  2:428008/1‑139 (MQ=255)                            gagaTCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCg                                                                                                <  1:747094/139‑1 (MQ=255)                                  cGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGAcc                                                                                          >  1:427977/1‑139 (MQ=255)                                    ccATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAAtt                                                                                                               <  2:1235309/116‑1 (MQ=255)                                    ccATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAAtt                                                                                                               >  1:1235309/1‑116 (MQ=255)                                    ccATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        <  1:15219/139‑1 (MQ=255)                                            cGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTg                                                                                             <  2:1184385/126‑1 (MQ=255)                                            cGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTg                                                                                             >  1:1184385/1‑126 (MQ=255)                                              aTTAACGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCa                                                                              >  2:189743/1‑139 (MQ=255)                                                  tCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTc                                                                          <  1:640906/139‑1 (MQ=255)                                                  tCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTc                                                                          <  2:1051505/139‑1 (MQ=255)                                                             gAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAg                                                               >  2:531363/1‑139 (MQ=255)                                                                   gACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTa                                                         <  2:427977/139‑1 (MQ=255)                                                                     cccGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           >  2:107201/1‑71 (MQ=255)                                                                     cccGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           <  1:107201/71‑1 (MQ=255)                                                                        gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAAtt                                                                                                               <  1:99954/80‑1 (MQ=255)                                                                        gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAAtt                                                                                                               >  2:99954/1‑80 (MQ=255)                                                                        gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        >  1:533657/1‑103 (MQ=255)                                                                        gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        <  2:533657/103‑1 (MQ=255)                                                                        gACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTg                                                    >  1:677185/1‑139 (MQ=255)                                                                         aCAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGc                                                   >  2:118296/1‑139 (MQ=255)                                                                             cggcgTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACtt                                               >  2:1215464/1‑139 (MQ=255)                                                                              ggcgTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTc                                              >  2:167441/1‑139 (MQ=255)                                                                                     cGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCg                                       <  1:118296/139‑1 (MQ=255)                                                                                       cAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGTGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAg                                     <  2:1334093/139‑1 (MQ=255)                                                                                       cAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAg                                     >  1:190916/1‑139 (MQ=255)                                                                                          tCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           <  2:112665/50‑1 (MQ=255)                                                                                          tCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           >  1:112665/1‑50 (MQ=255)                                                                                               tggtgCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAAc                                                                   <  2:1152/101‑1 (MQ=255)                                                                                               tggtgCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAAc                                                                   >  1:1152/1‑101 (MQ=255)                                                                                                 gtgCATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        >  1:837938/1‑78 (MQ=255)                                                                                                 gagcctttCGATTTCCGTAAGCTGGACGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        <  2:837938/73‑1 (MQ=255)                                                                                                    cATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           <  1:963533/40‑1 (MQ=255)                                                                                                    cATTTCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCg                                                                                                                           >  2:963533/1‑40 (MQ=255)                                                                                                        tCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTa                                                         <  2:893007/102‑1 (MQ=255)                                                                                                        tCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTa                                                         >  1:893007/1‑102 (MQ=255)                                                                                                        tCGATTTCCGTAAGCTGGATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGc                    >  1:1083048/1‑139 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGCGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTa                                                         <  1:932093/85‑1 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGa                                                                                            <  1:1332844/50‑1 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGa                                                                                            >  2:1332844/1‑50 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGa                                                                 <  2:450281/77‑1 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGa                                                                 >  1:450281/1‑77 (MQ=255)                                                                                                                         gATGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTa                                                         >  2:932093/1‑85 (MQ=255)                                                                                                                          aTGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCgg  >  2:431835/1‑139 (MQ=255)                                                                                                                           tGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        <  2:541433/52‑1 (MQ=255)                                                                                                                           tGTCTTCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        >  1:541433/1‑52 (MQ=255)                                                                                                                               ttCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTg                                                                                             >  2:617596/1‑43 (MQ=255)                                                                                                                               ttCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTg                                                                                             <  1:617596/43‑1 (MQ=255)                                                                                                                               ttCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        <  1:1214382/48‑1 (MQ=255)                                                                                                                               ttCAGTTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACctg                                                                                        >  2:1214382/1‑48 (MQ=255)                                                                                                                                    |                                                                                                                                 ACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAGTGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGG  >  NC_000913/3992767‑3993026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑