Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,158,575 A→G Q29R (CAG→CGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,5750AG100.0% 282.6 / NA 77Q29R (CAG→CGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/35);  total (42/35)

TGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTA  >  NC_000913/4158441‑4158697
                                                                                                                                      |                                                                                                                          
tGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAgccggccg                                                                                                                        >  2:1369401/1‑139 (MQ=255)
        tACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAg                                                                                                                >  2:324357/1‑139 (MQ=255)
               cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCaa                                                                                                         >  2:456586/1‑139 (MQ=255)
                 ccATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAAt                                                                                                       >  2:716449/1‑139 (MQ=255)
                  cATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAAtt                                                                                                      >  2:1215931/1‑139 (MQ=255)
                    tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCg                                                                                                    >  2:1253556/1‑139 (MQ=255)
                    tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCg                                                                                                    >  2:155599/1‑139 (MQ=255)
                          aTCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCA‑TTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTg                                                                                             <  2:72847/139‑1 (MQ=255)
                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTa                                                                                                                 >  2:760981/1‑114 (MQ=255)
                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTa                                                                                                                 <  1:760981/114‑1 (MQ=255)
                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGt                                                                                                   >  1:1063294/1‑128 (MQ=255)
                                gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGt                                                                                                   <  2:1063294/128‑1 (MQ=255)
                                     tgaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTCGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   >  2:839260/2‑139 (MQ=255)
                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   >  1:577428/1‑139 (MQ=255)
                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   >  2:1268519/1‑139 (MQ=255)
                                     ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   <  1:1068456/139‑1 (MQ=255)
                                       aggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTg                                                                                 >  2:773251/1‑139 (MQ=255)
                                          atggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                              <  2:253074/139‑1 (MQ=255)
                                           tggatAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCg                                                                             <  1:1215931/139‑1 (MQ=255)
                                              atAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                          <  1:773251/139‑1 (MQ=255)
                                                aaTGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATg                                                                        >  1:509724/1‑139 (MQ=255)
                                                 aTGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGt                                                                       >  2:682704/1‑139 (MQ=255)
                                                   gAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTg                                                                     >  1:1113249/1‑139 (MQ=255)
                                                   gAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTg                                                                     >  1:942914/1‑139 (MQ=255)
                                                    aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                              <  1:534920/129‑1 (MQ=255)
                                                    aaTATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGc                                                                              >  2:534920/1‑129 (MQ=255)
                                                       atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCt                                                                   <  2:308507/137‑1 (MQ=255)
                                                       atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCt                                                                   >  1:308507/1‑137 (MQ=255)
                                                        ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGa                                                                >  1:810858/1‑139 (MQ=255)
                                                            tGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCgg                                                            >  2:700013/1‑139 (MQ=255)
                                                             gATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTgg                                                                                            <  1:1371258/106‑1 (MQ=255)
                                                             gATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTgg                                                                                            >  2:1371258/1‑106 (MQ=255)
                                                              aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                          >  2:183988/1‑123 (MQ=255)
                                                              aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                          <  1:183988/123‑1 (MQ=255)
                                                              aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                          >  2:1101933/1‑139 (MQ=255)
                                                                cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   >  1:1028764/1‑112 (MQ=255)
                                                                cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   <  2:1028764/112‑1 (MQ=255)
                                                                cTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCa                                                        <  2:942914/139‑1 (MQ=255)
                                                                 ttGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                       <  1:1369401/139‑1 (MQ=255)
                                                                   gAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGcc                                                     >  1:776015/1‑139 (MQ=255)
                                                                           ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                             >  1:860599/1‑139 (MQ=255)
                                                                           ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                             >  2:1152274/1‑139 (MQ=255)
                                                                           ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                             <  2:321404/139‑1 (MQ=255)
                                                                           ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCCTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                             >  1:832686/1‑139 (MQ=255)
                                                                                   tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                     <  2:1113249/139‑1 (MQ=255)
                                                                                   tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                     >  2:1262393/1‑139 (MQ=255)
                                                                                   tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                     <  1:1253556/139‑1 (MQ=255)
                                                                                         aaCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc                               <  1:1227191/139‑1 (MQ=255)
                                                                                            acCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCggg                            <  1:682704/139‑1 (MQ=255)
                                                                                              cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                              <  2:548109/119‑1 (MQ=255)
                                                                                              cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                              >  1:548109/1‑119 (MQ=255)
                                                                                              cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAcc                                    >  1:102947/1‑129 (MQ=255)
                                                                                              cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAcc                                    <  2:102947/129‑1 (MQ=255)
                                                                                               gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAg                                                                                                                <  2:1240674/52‑1 (MQ=255)
                                                                                               gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAg                                                                                                                >  1:1240674/1‑52 (MQ=255)
                                                                                               gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                       <  2:186422/109‑1 (MQ=255)
                                                                                               gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                       >  1:186422/1‑109 (MQ=255)
                                                                                               gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAt                         >  2:1102494/1‑139 (MQ=255)
                                                                                                ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGc                                                                                                           <  2:873251/56‑1 (MQ=255)
                                                                                                ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGc                                                                                                           >  1:873251/1‑56 (MQ=255)
                                                                                                ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCCTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                        <  2:832686/139‑1 (MQ=255)
                                                                                                     ttCGGCGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATgctgct                   <  2:577428/139‑1 (MQ=255)
                                                                                                          cGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAg                                                                                    >  1:1178598/1‑69 (MQ=255)
                                                                                                          cGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAg                                                                                    <  2:1178598/69‑1 (MQ=255)
                                                                                                          cGTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtggtgg              >  2:295345/1‑139 (MQ=255)
                                                                                                           gTGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGa             >  2:1232956/1‑139 (MQ=255)
                                                                                                            tGCGGCAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt            <  1:1101933/139‑1 (MQ=255)
                                                                                                                 cAGATTCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc       <  2:810858/139‑1 (MQ=255)
                                                                                                                     ttCCTGCCACGTTACCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGCCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGt   <  2:838284/139‑1 (MQ=255)
                                                                                                                      tCCTGCCACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTa  <  1:155599/139‑1 (MQ=255)
                                                                                                                           ccACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                                                       <  1:203761/49‑1 (MQ=255)
                                                                                                                           ccACGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGAt                                                                                       >  2:203761/1‑49 (MQ=255)
                                                                                                                             aCGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                          >  1:1319863/1‑76 (MQ=255)
                                                                                                                             aCGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                          <  2:1319863/76‑1 (MQ=255)
                                                                                                                              cGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAATCTGGAAGATGAGCTGGGCGt                                                                            >  2:870337/1‑57 (MQ=255)
                                                                                                                              cGTTAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGt                                                                            <  1:870337/57‑1 (MQ=255)
                                                                                                                                ttAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   <  1:1215599/48‑1 (MQ=255)
                                                                                                                                ttAGCCGGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                   >  2:1215599/1‑48 (MQ=255)
                                                                                                                                  agccggccGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                       <  2:710509/106‑1 (MQ=255)
                                                                                                                                  agccggccGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                       >  1:710509/1‑106 (MQ=255)
                                                                                                                                      |                                                                                                                          
TGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTA  >  NC_000913/4158441‑4158697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑