Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,189,084 C→T G1245G (GGC→GGT rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,0840CT100.0% 99.9 / NA 32G1245G (GGC→GGTrpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/14);  total (18/14)

AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAA  >  NC_000913/4188963‑4189215
                                                                                                                         |                                                                                                                                   
aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATaa                                                                                                                    <  2:1175750/139‑1 (MQ=255)
                  ccGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                                                  <  2:429951/139‑1 (MQ=255)
                            ggAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtg                                                                                        <  1:942619/139‑1 (MQ=255)
                                   ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa                                                                                                                           <  2:74711/97‑1 (MQ=255)
                                   ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa                                                                                                                           >  1:74711/1‑97 (MQ=255)
                                   ccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTa                                                                                 >  2:1048325/1‑139 (MQ=255)
                                              tctgcgtctgcgTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATc                                                                      >  2:935296/1‑139 (MQ=255)
                                                  cgtctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                                                  >  2:1051728/1‑107 (MQ=255)
                                                  cgtctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                                                  <  1:1051728/107‑1 (MQ=255)
                                                     ctgcgtGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg                                                               >  2:728900/1‑139 (MQ=255)
                                                                      tGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa                                                                                                                           <  2:93198/62‑1 (MQ=255)
                                                                      tGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTa                                                                                                                           >  1:93198/1‑62 (MQ=255)
                                                                       gTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACt                                                <  1:894042/136‑1 (MQ=255)
                                                                       gTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACt                                                >  2:894042/1‑136 (MQ=255)
                                                                              gTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg                                      <  1:728900/139‑1 (MQ=255)
                                                                                      gTTAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAgg                              <  1:1266586/139‑1 (MQ=255)
                                                                                       ttAACGAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGt                             >  2:240873/1‑139 (MQ=255)
                                                                                            gAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGAt                                                                                                                             >  1:1308063/1‑38 (MQ=255)
                                                                                            gAAGTACAGGACGTATACCGTCTGCAGGGTGTTAAGAt                                                                                                                             <  2:1308063/38‑1 (MQ=255)
                                                                                               gTACAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGGTGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATAct                     >  1:174847/1‑139 (MQ=255)
                                                                                                 aCAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATActct                   <  1:1048325/139‑1 (MQ=255)
                                                                                                  cAGGACGTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACtctc                  >  2:1332039/1‑139 (MQ=255)
                                                                                                        gTATACCGTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCa            >  2:1370529/1‑139 (MQ=255)
                                                                                                               gTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt                                                                   >  2:490171/1‑77 (MQ=255)
                                                                                                               gTCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt                                                                   <  1:490171/77‑1 (MQ=255)
                                                                                                                tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgttc                                                                                                <  2:1365484/47‑1 (MQ=255)
                                                                                                                tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTAtcgttc                                                                                                >  1:1365484/1‑47 (MQ=255)
                                                                                                                tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGTTGCGTa                                                                                 <  2:65083/62‑1 (MQ=255)
                                                                                                                tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGTTGCGTa                                                                                 >  1:65083/1‑62 (MQ=255)
                                                                                                                tCTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGAGTCAAGATCGCa    >  1:570296/1‑139 (MQ=255)
                                                                                                                 cTGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCaa   >  2:249659/1‑139 (MQ=255)
                                                                                                                  tGCAGGGTGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCaaa  >  2:127288/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                   
AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGCAAA  >  NC_000913/4188963‑4189215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: