Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,218,815	A→G	T74A (ACG→GCG)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,218,815	0	A	G	100.0%	177.9 / NA	50	T74A (ACG→GCG)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

GCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTC  >  NC_000913/4218686‑4218947                                                                                                                                  |                                                                                                                                     gcgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                             <  1:934098/139‑1 (MQ=255)  cgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                               >  2:28472/1‑136 (MQ=255)  cgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                               <  1:28472/136‑1 (MQ=255)          cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtac                                                                                                                    >  1:840268/1‑139 (MQ=255)                      gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                               <  2:663344/116‑1 (MQ=255)                      gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                               >  1:663344/1‑116 (MQ=255)                         gACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGc                                                                                                     <  2:653130/139‑1 (MQ=255)                              gCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGCCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTac                                                                                                >  2:921645/1‑139 (MQ=255)                                       ccAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTg                                                                                       <  2:840268/139‑1 (MQ=255)                                                    aaaaaCCGTATCCATCTTCACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTAcc                                                                          <  2:919166/139‑1 (MQ=255)                                                            tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTc                                                                  >  2:1229761/1‑139 (MQ=255)                                                                 aTCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGAt                                                             >  2:136203/1‑139 (MQ=255)                                                                   cTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTg                                                           >  1:1131046/1‑139 (MQ=255)                                                                      tACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                     >  1:505706/1‑78 (MQ=255)                                                                      tACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                     <  2:505706/78‑1 (MQ=255)                                                                         gATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                     <  1:511683/139‑1 (MQ=255)                                                                          atcatcACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCgg                                                                                          <  2:278233/101‑1 (MQ=255)                                                                          atcatcACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCgg                                                                                          >  1:278233/1‑101 (MQ=255)                                                                             atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCa                                                                                                                           <  2:395760/65‑1 (MQ=255)                                                                             atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCa                                                                                                                           >  1:395760/1‑65 (MQ=255)                                                                              tcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCttt                                                >  2:672095/1‑139 (MQ=255)                                                                                  gTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                            <  2:1054080/139‑1 (MQ=255)                                                                                     ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                             >  2:828745/1‑55 (MQ=255)                                                                                     ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                             <  1:828745/55‑1 (MQ=255)                                                                                     ggcctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                         <  1:921645/136‑1 (MQ=255)                                                                                       tctggtcTTGGAGCAACTGCGCTGCATTACT‑ACGGCCAAAATGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGc                                                                                   >  2:834413/1‑94 (MQ=255)                                                                                       tctggtcGTGGAGCAACTGCGCTGCATTACT‑ACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGc                                                                                   <  1:834413/94‑1 (MQ=255)                                                                                            tcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTa                                  >  2:1028948/1‑139 (MQ=255)                                                                                                            cTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGATAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTAt                       <  2:205021/134‑1 (MQ=255)                                                                                                            cTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGATAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTAt                       >  1:205021/1‑134 (MQ=255)                                                                                                             tGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGacc                 <  2:128555/139‑1 (MQ=255)                                                                                                               cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                   >  2:1218381/1‑103 (MQ=255)                                                                                                               cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                   <  1:1218381/103‑1 (MQ=255)                                                                                                               cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGATGCCGGATTAGCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGaccac               >  2:851630/1‑139 (MQ=255)                                                                                                                 ttACTAACGGCCAAAGTGCGGACGCGGCATTTTtactac                                                                                                                 <  2:1365771/39‑1 (MQ=38)                                                                                                                 ttACTAACGGCCAAAGTGCGGACGCGGCATTTTtactac                                                                                                                 >  1:1365771/1‑39 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGtt                                                                                     >  2:1222689/1‑59 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGtt                                                                                     <  1:1222689/59‑1 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                   <  2:629317/93‑1 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                   >  1:629317/1‑93 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                         >  1:937510/1‑103 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                         <  2:937510/103‑1 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                          <  2:329632/118‑1 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                          >  1:329632/1‑118 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa     >  2:1310049/1‑139 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa     >  2:789134/1‑139 (MQ=255)                                                                                                                         ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa     >  2:1168393/1‑139 (MQ=255)                                                                                                                            cAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                            <  2:28678/97‑1 (MQ=255)                                                                                                                            cAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                            >  1:28678/1‑97 (MQ=255)                                                                                                                            cAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTc  >  1:105813/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                     GCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTC  >  NC_000913/4218686‑4218947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑