Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,490,141 C→T *333* (TAG→TAA idnR ← transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,490,1410CT100.0% 218.1 / NA 67*333* (TAG→TAAidnRtranscriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/35);  total (32/35)

AAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCG  >  NC_000913/4490015‑4490276
                                                                                                                              |                                                                                                                                       
tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                             >  1:740376‑M2/113‑139 (MQ=255)
tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                             >  2:1145819‑M2/113‑139 (MQ=255)
tcccggcagtaaaaacagttctgtgtccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTg                                                                                                                             >  1:389295‑M2/113‑139 (MQ=255)
      cagtaaaaacagttctgtttccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGa                                                                                                                       <  1:70466‑M2/33‑1 (MQ=255)
                          ccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            <  1:694565‑M2/28‑1 (MQ=255)
                          ccggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            >  2:694565‑M2/87‑114 (MQ=255)
                            ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      <  2:28207‑M2/50‑1 (MQ=255)
                            ggcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      >  1:28207‑M2/85‑134 (MQ=255)
                             gcgtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAAc                                                                                                <  1:1145819‑M2/56‑1 (MQ=255)
                               gtgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              <  2:991373‑M2/58‑1 (MQ=255)
                                tgcgggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAgt                                                                                             >  2:1167946‑M2/81‑139 (MQ=255)
                                   gggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  2:389295‑M2/62‑1 (MQ=255)
                                   gggcaattaacaggggttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGAGACCCGAGGTCAACAGtgtt                                                                                          <  2:911215‑M2/62‑1 (MQ=255)
                                                   ttcactcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTa                                                                          >  1:783393‑M2/62‑139 (MQ=255)
                                                      actcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATaaa                                                                                                                   <  2:550597‑M2/37‑1 (MQ=255)
                                                      actcatgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATaaa                                                                                                                   >  1:550597‑M2/59‑95 (MQ=255)
                                                          atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                                               >  2:111749‑M2/55‑111 (MQ=255)
                                                          atgagatatcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACa                                                                                               <  1:111749‑M2/57‑1 (MQ=255)
                                                                 atcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCt                                                            <  2:783393‑M2/92‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 >  2:118441‑M2/47‑101 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGa                                                                                >  1:1205441‑M2/47‑118 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGa                                                                                <  2:1205441‑M2/72‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                           >  2:618246‑M2/47‑139 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                           <  2:740376‑M2/93‑1 (MQ=255)
                                                                  tcctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTc                                                           >  1:421837‑M2/47‑139 (MQ=255)
                                                                  tcctccagtttcctgcctagagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 <  1:118441‑M2/55‑1 (MQ=255)
                                                                    ctccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      >  1:867845‑M2/45‑94 (MQ=255)
                                                                    ctccaatttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      <  2:867845‑M2/50‑1 (MQ=255)
                                                                      ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              <  1:719921‑M2/58‑1 (MQ=255)
                                                                      ccagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAg                                                                                              >  2:719921‑M2/43‑100 (MQ=255)
                                                                       cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            >  2:283555‑M2/42‑69 (MQ=255)
                                                                       cagtttcctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGc                                                                                                                            <  1:283555‑M2/28‑1 (MQ=255)
                                                                             cctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGa                                                                                                              <  1:1057019‑M2/42‑1 (MQ=255)
                                                                             cctgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGa                                                                                                              >  2:1057019‑M2/36‑77 (MQ=255)
                                                                               tgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTggg                                              >  1:222790‑M2/34‑139 (MQ=255)
                                                                               tgcctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTggg                                              >  2:463943‑M2/34‑139 (MQ=255)
                                                                                 cctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                                            <  2:421837‑M2/108‑1 (MQ=255)
                                                                                 cctggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGggcg                                            <  1:618246‑M2/108‑1 (MQ=255)
                                                                                    ggagtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGcc                                         >  1:433062‑M2/29‑139 (MQ=255)
                                                                                      agtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCAt                                       <  1:1167946‑M2/113‑1 (MQ=255)
                                                                                       gtataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATc                                      <  1:1166220‑M2/114‑1 (MQ=255)
                                                                                         ataggcaacgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGTTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCg                                    <  2:433062‑M2/116‑1 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                         <  2:403308‑M2/47‑1 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATAccc                                                                                                         >  1:403308‑M2/17‑63 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      >  1:920112‑M2/17‑66 (MQ=255)
                                                                                                acgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAg                                                                                                      <  2:920112‑M2/50‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTg                                                                                                                        >  1:1130392‑M2/16‑47 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTg                                                                                                                        <  2:1130392‑M2/32‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                                         >  1:672739‑M2/16‑78 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgttg                                                                                         <  2:672739‑M2/63‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          <  2:872420‑M2/62‑1 (MQ=255)
                                                                                                 cgtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGtgtt                                                                                          >  1:872420‑M2/16‑77 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                                    >  2:725405‑M2/15‑66 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGt                                                                                                    <  1:725405‑M2/52‑1 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 <  2:1232885‑M2/55‑1 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCaa                                                                                                 >  1:1232885‑M2/15‑69 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg                                                         >  2:468466‑M2/15‑109 (MQ=255)
                                                                                                  gtccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAg                                                         <  1:468466‑M2/95‑1 (MQ=255)
                                                                                                    ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtt                                                                        <  2:1084722‑M2/80‑1 (MQ=255)
                                                                                                    ccggcaaagagaGAAGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTAtt                                                                        >  1:1084722‑M2/13‑92 (MQ=255)
                                                                                                                 aaGAGATAATAACTTAGAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAAt            <  1:1298883/139‑1 (MQ=255)
                                                                                                                 aaGAGAAAATAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAAt            >  1:223523/1‑139 (MQ=255)
                                                                                                                      aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTg                                                              >  2:1041423/1‑84 (MQ=255)
                                                                                                                      aaaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTg                                                              <  1:1041423/84‑1 (MQ=255)
                                                                                                                       aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggt                                                                                     >  2:1077260/1‑60 (MQ=255)
                                                                                                                       aaaTAACTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTgttgtggt                                                                                     <  1:1077260/60‑1 (MQ=255)
                                                                                                                           aaCTTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCg  <  2:222790/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                       
AAAACAGTTCTGTGTCCGGCGTGCGGGCAATTAACAGGGGTTCACTCATGAGATATCCTCCAGTTTCCTGCCTGGAGTATAGGCAACGTCCGGCAAAGAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAAATCTGATACCCGAGGTCAACAGTGTTGTGGTTGTGATCGTTATTTTTAATTTTGCTCAGCAACATTTGGGCGGCCATCCGACCAATATCAAAACGGGGAGTAATTACGCTGGCG  >  NC_000913/4490015‑4490276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: