Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,966	C→T	*427* (TAG→TAA)	yjiN ←	zinc‑type alcohol dehydrogenase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,966	0	C	T	100.0%	232.2 / NA	71	*427* (TAG→TAA)	yjiN	zinc‑type alcohol dehydrogenase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/33);  total (38/33)

TCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTG  >  NC_000913/4565834‑4566097                                                                                                                                     |                                                                                                                                    tCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTaaaaa                                                                                                                               <  1:813314/139‑1 (MQ=255)                            ccccGTTCCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGa                                                                                                                  <  1:838680/125‑1 (MQ=255)                            ccccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGa                                                                                                                  >  2:838680/1‑125 (MQ=255)                              ccGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTg                                                                                                  >  2:1053412/1‑139 (MQ=255)                                 tACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                         >  2:353684/1‑113 (MQ=255)                                 tACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                         <  1:353684/113‑1 (MQ=255)                                       gggAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGCTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCaa                                                                                         >  1:218891/1‑139 (MQ=255)                                          agaTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                          <  1:1000115/103‑1 (MQ=255)                                          agaTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGcc                                                                                                                          >  2:1000115/1‑103 (MQ=255)                                            aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGaa                                                                                                                 <  1:865606/110‑1 (MQ=255)                                            aTAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGaa                                                                                                                 >  2:865606/1‑110 (MQ=255)                                             tAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                           >  1:1150337/1‑131 (MQ=255)                                             tAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                           <  2:1150337/131‑1 (MQ=255)                                                   aaaGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAg                                                                             <  1:794337/139‑1 (MQ=255)                                                        ggCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                      >  1:341559/1‑93 (MQ=255)                                                        ggCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAgg                                                                                                                      <  2:341559/93‑1 (MQ=255)                                                         gCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                          >  1:230064/1‑120 (MQ=255)                                                         gCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                          <  2:230064/120‑1 (MQ=255)                                                                 attaCCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGc                                                               >  2:912529/1‑139 (MQ=255)                                                                     ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                                           <  1:444805/139‑1 (MQ=255)                                                                     ccATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAAc                                                           <  1:912529/139‑1 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGc                                                                                                    <  1:297077/95‑1 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGc                                                                                                    >  2:297077/1‑95 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                        >  2:1033015/1‑139 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                        >  1:276220/1‑139 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAg                                                        >  2:999606/1‑139 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCAATTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAAc                                                                                                                <  1:375225/83‑1 (MQ=255)                                                                        ttCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCAATTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAAc                                                                                                                >  2:375225/1‑83 (MQ=255)                                                                          cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                              >  1:257770/1‑131 (MQ=255)                                                                          cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGcc                                                              <  2:257770/131‑1 (MQ=255)                                                                          cTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCg                                                      >  1:1181571/1‑139 (MQ=255)                                                                            tttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                              <  2:370840/97‑1 (MQ=255)                                                                            tttAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGAc                                                                                              >  1:370840/1‑97 (MQ=255)                                                                                 tATGGCTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGtt                                               <  2:218891/139‑1 (MQ=255)                                                                                   tGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCg                                                                                                         >  1:219454/1‑79 (MQ=255)                                                                                   tGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCg                                                                                                         <  2:219454/79‑1 (MQ=255)                                                                                   tGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                             >  1:173653/1‑139 (MQ=255)                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTCGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                         >  1:210341/1‑61 (MQ=255)                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCg                                                                                                                         <  2:210341/61‑1 (MQ=255)                                                                                     gTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGaca                                           >  1:137924/1‑139 (MQ=255)                                                                                         aTTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGaa                                       >  2:673426/1‑139 (MQ=255)                                                                                           ttGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGagta                                     <  2:1181571/139‑4 (MQ=255)                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                                           >  1:8229/1‑49 (MQ=255)                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGc                                                                                                                           <  2:8229/49‑1 (MQ=255)                                                                                               tctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACt                                 >  2:32484/1‑139 (MQ=255)                                                                                                ctctctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTg                                <  1:999606/139‑1 (MQ=255)                                                                                                   tctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAg                             >  1:167124/1‑139 (MQ=255)                                                                                                    ctcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGa                            >  2:528581/1‑139 (MQ=255)                                                                                                     tcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                     >  1:1251353/1‑97 (MQ=255)                                                                                                     tcGCGGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                     <  2:1251353/97‑1 (MQ=255)                                                                                                        cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                            <  2:425782/103‑1 (MQ=255)                                                                                                        cgGTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCaa                                                            >  1:425782/1‑103 (MQ=255)                                                                                                          gTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                                     >  2:586067/1‑124 (MQ=255)                                                                                                          gTTAGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATa                                     <  1:586067/124‑1 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                           <  2:1318888/67‑1 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGc                                                                                           >  1:1318888/1‑67 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                   >  1:510432/1‑139 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                   <  2:747926/139‑1 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                   >  2:1156022/1‑139 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                   >  2:1289458/1‑139 (MQ=255)                                                                                                             aGCCACTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCg                   <  1:1053412/139‑1 (MQ=255)                                                                                                                  cTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATAtt              <  1:528581/139‑1 (MQ=255)                                                                                                                  cTTAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATAtt              <  2:276220/139‑1 (MQ=255)                                                                                                                   ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                     >  2:34480/1‑83 (MQ=255)                                                                                                                   ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATAc                                                                     <  1:34480/83‑1 (MQ=255)                                                                                                                   ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                             >  2:302428/1‑107 (MQ=255)                                                                                                                   ttAGTTTTTCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGa                                             <  1:302428/107‑1 (MQ=255)                                                                                                                         tttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                          >  2:374068/1‑56 (MQ=255)                                                                                                                         tttCATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCa                                                                                          <  1:374068/56‑1 (MQ=255)                                                                                                                            cATGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTAtt    >  1:902770/1‑139 (MQ=255)                                                                                                                              tGGATTTTTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTg  <  2:137924/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    TCGGTGACAAGTGACATGATTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAGTATGGTTTATTTGATCTCTCTCGCGGTTAGCCACTTAGTTTTTCATGGATTTCTAAAAATTGCCGAGGGGGAACAAGGCCGGGAGCTGCGACAGCAAATATAAAATTAGCCCAATACAACCGCCAACCAGCGTACCGTTGACACGAATAAACTGCAGATCTTTGCCGATATTTAACTCTATTTG  >  NC_000913/4565834‑4566097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑