Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,612,289	G→A	*54* (TAG→TAA)	ytjA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,289	0	G	A	100.0%	130.8 / NA	41	*54* (TAG→TAA)	ytjA	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

GTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCA  >  NC_000913/4612155‑4612418                                                                                                                                       |                                                                                                                                  gTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAAttt                                                                                                                               >  2:592257/1‑139 (MQ=255) gTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAAttt                                                                                                                               >  2:1258167/1‑139 (MQ=255)           tAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTa                                                                                                                     >  1:689391/1‑139 (MQ=255)                         cTTGGATTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCCGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       <  2:849979/139‑1 (MQ=255)                                ttGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCa                                                                                                <  1:1258167/139‑1 (MQ=255)                                 tggtggTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       <  1:1083565/131‑1 (MQ=255)                                 tggtggTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       >  2:1083565/1‑131 (MQ=255)                                  ggtggtCTGGCCGGTACCGCTGCAGGCACAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGt                                                                                              >  2:54697/1‑139 (MQ=255)                                     ggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           <  1:592257/139‑1 (MQ=255)                                     ggtCTGGCCGGTACCGCTGCAGGCACAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           <  1:54697/139‑1 (MQ=255)                                           gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      >  1:1225946/1‑138 (MQ=255)                                           gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      <  2:1225946/138‑1 (MQ=255)                                                tACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCt                                                                                >  1:718525/1‑139 (MQ=255)                                                 aCCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       <  2:708469/115‑1 (MQ=255)                                                 aCCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATcc                                                                                                       >  1:708469/1‑115 (MQ=255)                                                     cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGc                                                                           >  2:1054674/1‑139 (MQ=255)                                                               aGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                                  >  1:621367/1‑90 (MQ=255)                                                               aGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGa                                                                                                                  <  2:621367/90‑1 (MQ=255)                                                                      aTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAataata                                                          <  2:718525/139‑1 (MQ=255)                                                                              tGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGt                                                  >  1:406431/1‑139 (MQ=255)                                                                                tcgtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTa                                                <  2:504157/139‑1 (MQ=255)                                                                                      ggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt                                          <  2:1147033/139‑1 (MQ=255)                                                                                                 ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            >  1:171155/1‑94 (MQ=255)                                                                                                 ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                            <  2:171155/94‑1 (MQ=255)                                                                                                 ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcac                               >  1:754066/1‑139 (MQ=255)                                                                                                 ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcac                               >  1:1017567/1‑139 (MQ=255)                                                                                                      ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc                                 >  1:301659/1‑132 (MQ=255)                                                                                                      ggTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc                                 <  2:301659/132‑1 (MQ=255)                                                                                                       gTGAGTTTGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACaaa                         <  2:406431/139‑1 (MQ=255)                                                                                                              tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           >  1:604200/1‑66 (MQ=255)                                                                                                              tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                                                           <  2:604200/66‑1 (MQ=255)                                                                                                              tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      >  2:1250381/1‑71 (MQ=255)                                                                                                              tGTTCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      <  1:1250381/71‑1 (MQ=255)                                                                                                                 tCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      >  2:1326413/1‑68 (MQ=255)                                                                                                                 tCATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                      <  1:1326413/68‑1 (MQ=255)                                                                                                                  cATGGGCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGc              <  2:689391/139‑1 (MQ=255)                                                                                                                     gggCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCaga           >  2:462248/1‑139 (MQ=255)                                                                                                                      ggCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                    <  2:501552/97‑1 (MQ=255)                                                                                                                      ggCCGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                    >  1:501552/1‑97 (MQ=255)                                                                                                                         cGAAAACGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTg       <  2:1017567/139‑1 (MQ=255)                                                                                                                              aCGACCCTAAATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCa  <  1:1054674/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  GTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCA  >  NC_000913/4612155‑4612418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑