Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 47,776 | G→A | *177* (TAG→TAA) S3N (AGC→AAC) |
kefF → kefC → |
potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 47,776 | 0 | G | A | 100.0% | 124.3 / NA | 39 | *177* (TAG→TAA) S3N (AGC→AAC) | kefF kefC | potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/19); total (20/19) |
GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGG > NC_000913/47652‑47893 | ggCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTc > 2:1344626/1‑139 (MQ=255) gAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgc < 1:1344626/139‑1 (MQ=255) aaCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg < 1:1094395/139‑1 (MQ=255) aaCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg < 1:110370/139‑1 (MQ=255) cACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTAtc > 2:123410/1‑139 (MQ=255) cACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTAtc > 2:325231/1‑139 (MQ=255) cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt > 2:22292/1‑139 (MQ=255) ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAc > 1:701660/1‑139 (MQ=255) ttGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAt < 2:701660/139‑1 (MQ=255) tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg < 1:1300027/100‑1 (MQ=255) tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg > 2:1300027/1‑100 (MQ=255) tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGG‑CCATCATGGATAACCATACGCTGATTCAGgcg > 2:896425/1‑99 (MQ=255) tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGG‑CCATCATGGATAACCATACGCTGAATCAGgcg < 1:896425/99‑1 (MQ=255) cgacgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTAt < 1:370625/104‑1 (MQ=255) cgacgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTAt > 2:370625/1‑104 (MQ=255) cgacgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt < 1:123410/139‑1 (MQ=255) ggCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt < 1:22292/139‑1 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 1:518330/80‑1 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 2:518330/1‑80 (MQ=255) cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc > 1:1015730/1‑139 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGAt < 1:620369/59‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGAt > 2:620369/1‑59 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 2:180758/1‑79 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 2:988280/79‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 1:1079744/79‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 2:1079744/1‑79 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc > 1:988280/1‑79 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc < 1:180758/79‑1 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg > 2:1200961/1‑139 (MQ=255) gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg < 1:325231/139‑1 (MQ=255) gCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTgg > 1:426793/1‑139 (MQ=255) ggaggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc < 1:668709/122‑1 (MQ=255) ggaggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc > 2:668709/1‑122 (MQ=255) ggCCCATCATGGATAACCATGCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt > 2:930031/1‑65 (MQ=255) ggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt < 1:930031/65‑1 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTAtct > 2:1110473/1‑42 (MQ=255) catcatGGATAACCATACGCTGATTCAGGCGCTGATTTAtct < 1:1110473/42‑1 (MQ=255) aTAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc < 1:580396/58‑1 (MQ=255) aTAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc > 2:580396/1‑58 (MQ=255) | GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGG > NC_000913/47652‑47893 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |