| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_010468 | 4,287,467 | A→C | G109G (GGA→GGC) | ECOLC_RS21005 → | class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_010468 | 4,287,467 | 0 | A | C | 99.3% | 5351.2 / NA | 1393 | G109G (GGA→GGC) | ECOLC_RS21005 | class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/10); new base C (680/701); total (680/713) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 1393 total aligned reads displayed.
CCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGAACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGACGCCGGGTAAACTG > NC_010468/4287171‑4287764 | tCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACcgc > 1:5765200/1‑298 (MQ=255) tCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACcgc < 1:802928/298‑1 (MQ=255) ccGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGat < 1:6043013/299‑1 (MQ=255) gggAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTTCTGCCGACCTGCCAGGATACCGGCACCGCGatcatcct > 1:2776848/1‑297 (MQ=255) gggAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCAtcgt > 2:6560796/1‑299 (MQ=255) gAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCAtcgtcg > 2:613769/1‑299 (MQ=255) aaaGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCgg < 2:4468423/299‑1 (MQ=255) aaGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCgg > 1:1900444/1‑298 (MQ=255) tACCGAATACTATCTACCACCTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAg < 2:4596048/299‑1 (MQ=255) cGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTc > 1:483917/1‑299 (MQ=255) tCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCCCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCgt < 1:1102558/297‑1 (MQ=255) ccTTCCGAGTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGACCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCgg < 2:6679896/298‑1 (MQ=255) cTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGt < 1:5168374/299‑1 (MQ=255) ccGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGc > 2:2312166/1‑299 (MQ=255) aCGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGtga < 2:3088722/298‑1 (MQ=255) cGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGtgatg > 2:1188105/1‑299 (MQ=255) aGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATgaag > 1:885331/1‑298 (MQ=255) cGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGaagaag > 1:6170702/1‑299 (MQ=255) tGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAgcgc > 1:3588440/1‑299 (MQ=255) ccGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGCAAAAAAGGTCAGCGCGGGTGGACCGGTGGGCGGT‑ATGAAGAAgcgcac > 2:4291700/1‑297 (MQ=255) gACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTc > 1:6618838/1‑299 (MQ=255) gACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTc < 1:4724885/299‑1 (MQ=255) cGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAg > 1:4050151/1‑299 (MQ=255) gACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAg < 1:5498862/298‑1 (MQ=255) gCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAGCTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTc < 2:1115248/299‑1 (MQ=255) aaaCCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCCCCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCta > 2:2447530/1‑298 (MQ=255) aaaCCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGCAGCGCTGTCTAAAGGCGCCta > 2:5446695/1‑298 (MQ=255) cATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAaca < 1:1005718/299‑1 (MQ=255) aTCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAacac < 2:776689/299‑1 (MQ=255) aaGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATc < 1:5935113/299‑1 (MQ=255) aGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATc > 1:4344163/1‑298 (MQ=255) gcggAACCAGAAGCCCTGACCCTGCTGGCGCATCAAGCCTTTCACGACGCTTCTTTCAGTCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGa < 2:3193708/297‑1 (MQ=255) gAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCt > 1:3717906/1‑299 (MQ=255) cccTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGTTGAAGAAGCGCTGTCGAAAGCCGTCTATACCACTTATATCGAAGATAACCTtcgc > 2:4117612/1‑299 (MQ=255) cccTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTca < 1:3821125/299‑1 (MQ=255) ccTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTca > 2:1884100/1‑298 (MQ=255) gctgGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATACCACTTATATCGAAGGTAACCTGCGCTATTCACAg > 2:479043/1‑298 (MQ=255) tgGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAACGGCGTCTATAACCCTTATATCGACGATACCCTGCGCTATTCACAGAAt > 2:2646291/1‑299 (MQ=255) gcgcAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATgc > 2:2100432/1‑299 (MQ=255) aGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTg > 2:6354684/1‑298 (MQ=255) ttCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATg > 2:6009210/1‑299 (MQ=255) ttCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATg > 2:1759621/1‑299 (MQ=255) acgCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACaa > 2:5173216/1‑298 (MQ=255) acgCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACa > 1:982135/1‑297 (MQ=255) tcttTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGt > 1:2920044/1‑299 (MQ=255) tCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCaa > 1:5076134/1‑298 (MQ=255) tCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCaa < 1:3374342/298‑1 (MQ=255) cATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAaca > 1:4256675/1‑299 (MQ=255) aTGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACGGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGGCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAacac > 1:1211946/1‑299 (MQ=255) aTGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAaca < 2:2570236/298‑1 (MQ=255) cGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCAc > 2:370010/1‑299 (MQ=255) ccAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAAcctgcctg < 2:4697062/299‑1 (MQ=255) aGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGca < 1:6354684/299‑1 (MQ=255) gAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCCCTGGACATGTACAAAGCGGTCAACACCGGCACTAACCGGCCTGca > 2:3341746/1‑298 (MQ=255) ggTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCg < 1:1385035/299‑1 (MQ=255) gTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGa < 2:1980364/299‑1 (MQ=255) tCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGAc < 2:3582760/299‑1 (MQ=255) gcgGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCt < 2:2788906/299‑1 (MQ=255) gcgGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCt < 1:731510/299‑1 (MQ=255) cgGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTg < 2:5737917/299‑1 (MQ=255) gcattgattcACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTa < 2:4374155/292‑1 (MQ=255) aTTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTAcgc > 1:3467085/1‑299 (MQ=255) ttcttcACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTAcgcg > 1:3949330/1‑299 (MQ=255) aCGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGa < 1:2357838/298‑1 (MQ=255) cAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGAt < 1:4630284/299‑1 (MQ=255) aGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGt < 1:3042635/299‑1 (MQ=255) gCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTa < 2:885331/299‑1 (MQ=255) ccAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTa > 2:4993873/1‑298 (MQ=255) aGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTa > 2:5321629/1‑296 (MQ=255) cGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGAt < 2:1346260/299‑1 (MQ=255) gAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGAtt > 2:3447918/1‑299 (MQ=255) aaaCGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTcc > 1:3184685/1‑299 (MQ=255) aaCGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAACGAGGTCAACACAGGCTCTAACCTGCCTGCACAAATCGACCTGTACGCGGGAGATGGCGATGAGTACAGATTCCt > 2:488668/1‑299 (MQ=255) aCGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCtt < 1:1998720/299‑1 (MQ=255) aCGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCtt < 1:4426832/299‑1 (MQ=255) aCAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTttgc > 1:2474290/1‑299 (MQ=255) cGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTttgcgttgcg > 1:4963885/1‑298 (MQ=255) gTGGCGCTGCAAGCCTTAAGAAGCGCCGAAGTCGCTGCCAAAGGCGTGCTGCAGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGaa < 2:5585125/299‑1 (MQ=255) tGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGa > 1:1729343/1‑297 (MQ=255) aaTTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAggcggcggc < 2:1575922/299‑1 (MQ=255) ttCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGctc < 1:5362737/299‑1 (MQ=255) aGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCcaa > 1:4170876/1‑299 (MQ=255) aaCACCGGAAGCCCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCcaacaa < 2:2924531/299‑1 (MQ=255) cTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACaaaa < 1:1807713/299‑1 (MQ=255) aTCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTAtct > 1:1611247/1‑299 (MQ=255) gCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACTAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTa > 1:2674783/1‑299 (MQ=255) cTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTAc < 1:1480542/299‑1 (MQ=255) cAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAgg < 1:5631100/299‑1 (MQ=255) cAAAGGCGTGCTGCCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTAGATCGCG‑GTCGTTGATGTATCATTACAGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCACTGCCAACAACACCTATCTCTACCAg > 2:1465788/1‑298 (MQ=255) cAAAGGCGTGCTGCCGACCTGCCAGGATACCAGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAgg < 2:3873198/299‑1 (MQ=255) ctgcCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAg < 1:2575558/298‑1 (MQ=255) ctgcCGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAg < 1:5260977/298‑1 (MQ=255) ccGACCTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGAGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGctct < 2:3994406/299‑1 (MQ=255) actggcaaGGATACCGGCACCGCGAGCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGAGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTctgct < 2:5083353/292‑1 (MQ=255) cgaccAGGAGACCTGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGGCGAAAGGCGTCTATAACACTGATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGACTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGa < 2:164350/296‑1 (MQ=255) cTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGa < 2:5890268/299‑1 (MQ=255) cTGCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGa < 1:6490649/299‑1 (MQ=255) gCCAGGATACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGACg > 2:6458573/1‑299 (MQ=255) ccAGGATACCGGAACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGAc < 1:2129967/297‑1 (MQ=255) ccGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGACGCCGGGTaaa < 1:5864165/299‑1 (MQ=255) | CCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGATTACGTTAGCGTTGCCGACTTCGACGGCGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACCCTGCTGGCGCAGCAAGCCTTTCACGACGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTCGCGGCTATTCTTCACGATCCAGAAGCCAGCGAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCTGCCAAAGGCGTGCTGCCGACCTGCCAGGATACCGGAACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGT‑GGCGGTGATGAAGAAGCGCTGTCGAAAGGCGTCTATAACACTTATATCGAAGATAACCTGCGCTATTCACAGAATGCGGCGCTGGACATGTACAAAGAGGTCAACACCGGCACTAACCTGCCTGCACAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAGATTCCTTTGCGTTGCGAAAGGCGGCGGCTCTGCCAACAAAACCTATCTCTACCAGGAAACCAAAGCTCTGCTGACGCCGGGTAAACTG > NC_010468/4287171‑4287764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |