BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	2,798,223	G→T	7.8%	C135F (TGC→TTC)	alaE →	L‑alanine exporter AlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	2,798,223	0	G	T	7.8%	539.9 / 6.5	178	C135F (TGC→TTC)	alaE	L‑alanine exporter AlaE
Reads supporting (aligned to +/- strand): ref base G (57/104); new base T (14/2); total (72/106)
Fisher's exact test for biased strand distribution p-value = 6.53e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

ATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGT  >  NC_007779/2797987‑2798459

aTGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTgcc                                                                                                                                                                                                                                               <  1:345375/239‑1 (MQ=255)

 tGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCCTCAGATTATGGC‑GGCGGTCAGGTCAAACATCGGTG‑TTTC‑GATGTTGATGGGGGCGGG‑TTATGGGC‑CATTCCT‑AGATTA‑TTgcc                                                                                                                                                                                                                                               >  1:250897/1‑238 (MQ=255)

     cATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGccgccg                                                                                                                                                                                                                                           <  2:1559560/238‑1 (MQ=255)

     cATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGa                                                                                                                                                                                                                                          <  1:1115110/239‑1 (MQ=255)

      aTACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGAc                                                                                                                                                                                                                                         <  1:1863492/239‑1 (MQ=255)

              tGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGTAATTTAAACATTGTTGTTTTC‑GTTTTTG‑TGGGGGGGGG‑TTTTGGCTGACTTCTC‑CCTTTT‑TTGCCCCCGGCTGTTTaa                                                                                                                                                                                                                                  >  2:863578/1‑239 (MQ=255)

              tGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGCAGGTCAAACATCGGTG‑TTTT‑GATGTTGATGGGGGGGGG‑TTTTGGGG‑AATTCCC‑CGGTTA‑TTTCCGCCGGCTGTTTtaa                                                                                                                                                                                                                                 >  1:31478/1‑239 (MQ=255)

                 aCCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGt                                                                                                                                                                                                                               <  1:739463/238‑1 (MQ=255)

                   cGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAg                                                                                                                                                                                                                            <  1:668088/239‑1 (MQ=255)

                     tGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCCTCAGATTATGGC‑GGCGGTCAGTTCCCACAACGGTG‑TTTG‑GTTTTTGATTGGGGGGGTGTTTTGGC‑‑ACCTCCTCCGATTATTTGCCTCAG‑CTGTTTAAAggtcac                                                                                                                                                                                                                           >  2:1577627/1‑234 (MQ=255)

                      gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGG‑TTATGGCT‑ACTTCCT‑CGATTT‑TTTCCGCCGGCTGTTTAAAGTCAG‑CCg                                                                                                                                                                                                                        >  1:1366473/1‑239 (MQ=255)

                      gATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGG‑TTATGGCT‑ACTTTCT‑CTATTA‑TTTTCGCCGACTGTTTAAAGTCAG‑CCg                                                                                                                                                                                                                        >  1:1614793/1‑239 (MQ=255)

                        tCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGtt                                                                                                                                                                                                                      <  1:2014059/239‑1 (MQ=255)

                          tGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGccgccg                                                                                                                                                                                                                                           <  1:1337390/217‑1 (MQ=255)

                              tttGCGCGCGGCCCGCAAAGTTTGCCCGGCGGGCTGGATAAAAAATCTTGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACcagc                                                                                                                                                                                                                <  2:232496/237‑1 (MQ=255)

                              tATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACcagc                                                                                                                                                                                                                <  2:2087135/239‑1 (MQ=255)

                              tATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGCCGGTTCAACCTAGTTGTTTTC‑TATTTTATTGGGGGGGGT‑TT‑TGGCA‑CCTTCCT‑CCTTTT‑TTTCCCCCGCCTGTTTTAAGTCCG‑CCCTTACcac                                                                                                                                                                                                                 >  2:903427/1‑237 (MQ=255)

                                  cgcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGGTCAAAAATTCTTG‑TTTT‑GATGTTGATGGGGGCGGG‑TTTTGGGT‑ATTTCTTCCGATTT‑TGTCCCCCCACCTTTTAAAGTCAC‑CGGGTAcccacagt                                                                                                                                                                                                             >  2:256357/1‑233 (MQ=255)

                                   gcgcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGt                                                                                                                                                                                                            <  1:1020666/238‑1 (MQ=255)

                                     gcgGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑aa                                                                                                                                                                                                         <  1:977097/238‑1 (MQ=255)

                                         cACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGc                                                                                                                                                                                                     <  1:1367208/238‑1 (MQ=255)

                                              aaaGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGAc                                                                                                                                                                                                <  1:1896958/238‑1 (MQ=255)

                                                   tAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGCAGTTCAAACAACGGTT‑TTTCGGCTTTGGTTGGGGGGGGT‑TTTTGGCT‑CTTCCCC‑CGTTTT‑TTGCC‑CCGGCTGGTTTAAGTCCGCCCGTTACCACAGGGC‑AAAACCCTgccact                                                                                                                                                                                             >  2:2011939/1‑232 (MQ=255)

                                                      cccGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGG‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGGTACCAGCAGGG‑AAAAGCCTGACTC‑TTCtttt                                                                                                                                                                                      >  1:625107/1‑239 (MQ=255)

                                                          tCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGCGGGGGGTCAG‑TCAAACCACGTTG‑TTTT‑GGTGTTGGTTGGGGCGGG‑TTGAGGCC‑TCTTTCT‑TCGCTA‑TTGTAGTCGACTGTTTTAAATCAG‑CCGGTTCCACCCGGTCAAATGCCT‑ACTCTTTCTTTTcc                                                                                                                                                                                    >  2:1268556/1‑237 (MQ=255)

                                                           cGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGa                                                                                                                                                                                 <  1:690783/239‑1 (MQ=255)

                                                                    tAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCg                                                                                                                                                                        <  2:173444/239‑1 (MQ=255)

                                                                                        cTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTGTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTgg                                                                                                                                                    <  1:745145/239‑1 (MQ=255)

                                                                                             ttATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCg                                                                                                                                               <  1:1577627/239‑1 (MQ=255)

                                                                                             ttATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCg                                                                                                                                               <  1:923572/239‑1 (MQ=255)

                                                                                               aTGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGc                                                                                                                                              >  1:1044159/1‑238 (MQ=255)

                                                                                                      gTTCCAGTCCCCGGTGTATGTGGGGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaa                                                                                                                                       <  2:738595/238‑1 (MQ=255)

                                                                                                        tgcAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTAGGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                     <  2:2088083/236‑1 (MQ=255)

                                                                                                        tCCGGTCACCGGAGTATGTGGCGAGCTTGTTAGTGGTGGGCGCGGACTGGCATCAGATTAGGGC‑GGCGGTCAGTTCAAACATCGTTT‑TTTC‑GATGTTGATGGGGGCGGT‑TTATTGCT‑TCTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGAATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                     <  2:1077294/238‑1 (MQ=255)

                                                                                                        tCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                     <  1:194658/238‑1 (MQ=255)

                                                                                                        tCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                     <  1:758890/238‑1 (MQ=255)

                                                                                                        tCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                     <  1:2057097/238‑1 (MQ=255)

                                                                                                          cAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAt                                                                                                                                  <  1:1760268/239‑1 (MQ=255)

                                                                                                            gTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGGCAGTTCAAAAATCGGTG‑TTTC‑GCTTTTTATGGGGGGGGG‑TTTTGGGG‑ACTTTCT‑CCGTTT‑TTTTCGCCCGCTGTTTTAAATCAG‑CCGGTTACAGCCGGG‑AAAAACCTCAATC‑CTCTTTTTTCGGCTGGCGTCCCCCGCCGCGGCTTTTACTGCGTTCTCCCCATAAACCacta                                                                                                                                >  2:1944996/1‑236 (MQ=255)

                                                                                                              cACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAtt                                                                                                                                 <  1:1013327/236‑1 (MQ=255)

                                                                                                               aCCGGTGTATGTGGGGATCTTGTTAGTGGTGGGCTGCGAGTGGCATGAGATTGTGGC‑GTATGTCAGTTCAAACATCGTTG‑TTTC‑GTTGTTGATGGGTGCGGT‑TTATGGCT‑ACTTCCT‑CAATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTTGCATCGTCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCaaa                                                                                                                             <  2:1360385/239‑1 (MQ=255)

                                                                                                                ccGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAAt                                                                                                                            <  1:924059/239‑1 (MQ=255)

                                                                                                                 cGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATa                                                                                                                           <  1:857681/239‑1 (MQ=255)

                                                                                                                  ggTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGGCTGTTTTAAGTCAG‑CCGGTACCAGCAGGT‑AAAAGCCTGGCTC‑TTCTTTTCCCGACTTGCATCGCCCGGCGCGGCCTTTAATGGCTTTCCCAAATAAAACCTTTCAATaa                                                                                                                          >  1:714441/1‑239 (MQ=255)

                                                                                                                  ggTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATaa                                                                                                                          <  1:903427/239‑1 (MQ=255)

                                                                                                                       aTGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc                                                                                                                     <  2:660927/239‑1 (MQ=255)

                                                                                                                                tCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTAGGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCaa                                                                                                             <  2:810578/238‑1 (MQ=255)

                                                                                                                                   tGTTAGTGGTGGGCGCAGATTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCTAATAAACCATTCAAATAACGTTCAAGCGCAACAcg                                                                                                         <  1:479682/239‑1 (MQ=255)

                                                                                                                                   tGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcg                                                                                                         <  2:1044159/239‑1 (MQ=255)

                                                                                                                                   tGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcg                                                                                                         <  1:1973494/239‑1 (MQ=255)

                                                                                                                                       aggtgtggGCGCAGACTGGAATCAGATTATGGC‑GCCGGTCAGCTCAATCATCTTTG‑TGTC‑GATGTTGATCGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TGCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAATCGCAACACGCGaa                                                                                                     <  2:1895720/235‑1 (MQ=255)

                                                                                                                                          ggtggGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc                                                                                                    <  1:581623/237‑1 (MQ=255)

                                                                                                                                          ggtggGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTa                                                                                                  <  1:1641134/239‑1 (MQ=255)

                                                                                                                                             gggCTCAGTCTGGCATCAGATTGTGGC‑GGCGGTCAGTTCAAACCTCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAg                                                                                               <  1:770080/239‑1 (MQ=255)

                                                                                                                                                cgcAGACGGGCATCAGTTTATGGG‑GTAGGTTAGTCCGATGATCGTTG‑TTTC‑GATGTTGTCGGTGGCCGT‑TGGTGGCC‑ACTTCCT‑CGATAT‑CAGCCGTCTATTATTTAAAGCCGG‑CGCTTACCAGCAGGT‑AAATGCATGATCA‑TACTCCGTGCGACTGCCGACTCGTGACGCTTTTTTAACTGGCTTCGGCAAATAAACCATTCAAATAACGTTCAAGCGTAACACGCGAACTAAAggc                                                                                             <  2:95709/238‑3 (MQ=255)

                                                                                                                                                   aGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATg                                                                                          <  1:1621985/238‑1 (MQ=255)

                                                                                                                                                      cTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                                        <  2:433271/237‑1 (MQ=255)

                                                                                                                                                      cTGGCATCAGATTATGGC‑GGCGGTCAGCTCAAACAACGCTG‑GGTC‑GACCTTGATCGGGGGGGT‑TTTTTCGC‑AATTCCT‑CGATTC‑TTTGCGCCGAGTGTTTTAAGTCTG‑CCCTTTCCACACCGT‑AAAAGCCTTCCTC‑TTCCTTTAGCGCCTGGCATCGCCAGTCGGGCCTTTTACAGGCCTGGCCAAAAAAACCATTTAACTAAACTTTAATACCAACACACCAACCAAAGCCacgtcgg                                                                                      >  2:1005180/1‑233 (MQ=255)

                                                                                                                                                       tGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                     <  1:1694163/239‑1 (MQ=255)

                                                                                                                                                             cggATTATGGC‑GGCGGTCAGTTCAAACTTCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGt                                                                               <  2:1524336/237‑1 (MQ=255)

                                                                                                                                                             cAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGt                                                                               <  1:432879/239‑1 (MQ=255)

                                                                                                                                                                 ttATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAATCCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt                                                                            <  1:266629/238‑1 (MQ=255)

                                                                                                                                                                   aTGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGGCAG‑CCGGTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGc                                                                          >  1:1032928/1‑238 (MQ=255)

                                                                                                                                                                    tGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcg                                                                                                         <  2:1606168/206‑1 (MQ=255)

                                                                                                                                                                      ggggggggtaggTTCAAACATCGTTT‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCg                                                                       <  2:351690/228‑1 (MQ=255)

                                                                                                                                                                           cggTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGGCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtc                                                                   >  1:109983/1‑238 (MQ=255)

                                                                                                                                                                               cAGTTCAATCATCGTTG‑TTTC‑GATGTTGAAGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCGTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctc                                                               <  1:1928504/238‑1 (MQ=255)

                                                                                                                                                                                atttCAAGCGTCTTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGGT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCATGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATATCGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctcgc                                                             <  2:368952/237‑1 (MQ=255)

                                                                                                                                                                                   tCAAACATCGTTG‑TTTC‑GATGTTGATGGGTGCTGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGGCTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAATCCATGCGGGATACCGTAATGCTCGTGtcgctcgc                                                             <  1:1839733/236‑1 (MQ=255)

                                                                                                                                                                                       aCATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCCTTCAAATAACGTTCAAGCGCAACACGCGAAATAAAGCCATGCGGGATAACGTAATGGTCACGTCGCCACCCTGCTaa                                                      >  2:113796/1‑239 (MQ=255)

                                                                                                                                                                                       aCATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                     <  2:1109538/208‑1 (MQ=255)

                                                                                                                                                                                       aCATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGACGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAATACGCGAACTAAAGCCATGCGGGATACCCTAATGCTCGTGTCGCTCGCCTGCTaa                                                      >  1:1150213/1‑239 (MQ=255)

                                                                                                                                                                                            gttg‑ttTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctcgc                                                             >  1:332223/1‑227 (MQ=255)

                                                                                                                                                                                                       aTGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt                                        >  1:2071422/1‑239 (MQ=255)

                                                                                                                                                                                                           tGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTg                                    <  1:2217526/239‑1 (MQ=255)

                                                                                                                                                                                                                  gggtgtgttATGGCT‑ACTTCCT‑GGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCTGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGTCAGTCGCGTCGTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt                               <  1:1899433/231‑1 (MQ=255)

                                                                                                                                                                                                                    cGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGAGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc                           >  1:116284/1‑239 (MQ=255)

                                                                                                                                                                                                                    cGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGAATGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTTGCATCCCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCTAAACACGCGAACTAAAACCATGCGGGATACCGTAATTCTCGTGGCGCCCGGCTGCTAAATACAACGGAAATTTCTGGTAATGATc                           >  2:1181755/1‑239 (MQ=255)

                                                                                                                                                                                                                     ggT‑TTATGGTT‑CCTTCCT‑CGATAA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTGACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCTCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCTGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCg                          <  1:2177086/239‑1 (MQ=255)

                                                                                                                                                                                                                      gT‑TTATGGCT‑ACTTCCC‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCTCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCggg                                                                                      >  2:1643087/1‑178 (MQ=255)

                                                                                                                                                                                                                       t‑ttATGGCT‑TCTTCCT‑CGAGTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTCACCC‑TTCTTTTCGCGACTGGCATCGCCCGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAGTGCTGGTAATGATCGCa                        <  2:2204259/239‑1 (MQ=255)

                                                                                                                                                                                                                         ttATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa                       >  1:146425/1‑239 (MQ=255)

                                                                                                                                                                                                                         ttATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCa                        <  2:1894826/238‑1 (MQ=255)

                                                                                                                                                                                                                         ttATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCa                        <  1:1870977/238‑1 (MQ=255)

                                                                                                                                                                                                                                  aCTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCTG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATa                                                                                                                                        >  2:518907/1‑118 (MQ=255)

                                                                                                                                                                                                                                  aCTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATa                                                                                                                                        <  1:518907/118‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGCCTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGCCTC‑TTCTTTTCACGACTGGCCTCCCCCTTCGCGTCTTTAAATGGCTTCGCCAAATAAACCATTCCAATAACGTTCACGCGCAACACGCGAACTAAAGACATCCGTGATACCATAATGCCACGGTCGCGCGCCTGCTAACAACAACGGAAATTGCTGGTAATGAGCGCAAGCATTAata              >  2:1379374/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                     <  1:1263537/168‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                     >  2:1263537/1‑168 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata              >  1:1113943/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata              >  2:781358/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata              >  2:1914824/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata              >  1:554904/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGGGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATGGCAAGTTTTAata              >  2:1104880/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAACGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata              >  2:766380/1‑239 (MQ=255)

                                                                                                                                                                                                                                          cGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGaa        >  2:1167409/1‑239 (MQ=255)

                                                                                                                                                                                                                                               a‑ttGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg                                                                                        >  1:2230114/1‑154 (MQ=255)

                                                                                                                                                                                                                                                 ttGCCGCCGACTGTTTCAAGTCAG‑CCGTGACCAGCAGGT‑AAAAGACTGACTC‑GTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCTCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGt  <  2:2035643/239‑1 (MQ=255)

                                                                                                                                                                                                                                                  tGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAg                                                                                                                  >  2:1849241/1‑126 (MQ=255)

                                                                                                                                                                                                                                                  tGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAg                                                                                                                  <  1:1849241/126‑1 (MQ=255)

ATGGCCATACGGTATGTACCGTGATCTGTTTATGCGCGCGGCACGCAAAGTTAGCCCGTCGGGCTGGATAAAAAATCTGGCGGATATCCTGGCTTATGTGACGTTCCAGTCACCGGTGTATGTGGCGATCTTGTTAGTGGTGGGCGCAGACTGGCATCAGATTATGGC‑GGCGGTCAGTTCAAACATCGTTG‑TTTC‑GATGTTGATGGGGGCGGT‑TTATGGCT‑ACTTCCT‑CGATTA‑TTGCCGCCGACTGTTTAAAGTCAG‑CCGTTACCAGCAGGT‑AAAAGCCTGACTC‑TTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGT  >  NC_007779/2797987‑2798459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`