BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	3,181,275	C→T	12.1%	L24F (CTC→TTC)	zupT →	zinc transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	3,181,275	0	C	T	12.1%	194.7 / 3.2	71	L24F (CTC→TTC)	zupT	zinc transporter ZupT
Reads supporting (aligned to +/- strand): ref base C (5/57); new base T (6/3); total (11/60)
Fisher's exact test for biased strand distribution p-value = 2.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCC  >  NC_007779/3181040‑3181504

tGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCGG‑GCACGGTTATTTGTG‑GCGTTT‑TC‑GGCCTtcttg                                                                                                                                                                                                                                      >  2:1825770/1‑237 (MQ=255)

tGCTGGCATACGTGTCGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑GCACGCTTA‑TTGGC‑GCGTTTTTC‑GCCGCtctcc                                                                                                                                                                                                                                      >  1:1538274/1‑238 (MQ=255)

       aTACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑aaa                                                                                                                                                                                                                               <  1:1895929/238‑1 (MQ=255)

           gtgtTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAAccc                                                                                                                                                                                                                          <  1:161487/239‑1 (MQ=255)

               tGAAGACATGATGATGTCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGAGGGTG‑CCACGGTTA‑TTGGC‑CCGCTTATCTGGCGTCCT‑GTGC‑ATAAAccccctc                                                                                                                                                                                                                      >  1:296119/1‑235 (MQ=255)

                  aGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAAcc                                                                                                                                                                                                                   <  1:1505056/239‑1 (MQ=255)

                      atgatgATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGCTTA‑TTTGC‑GCGGTTTTC‑CCCGTTTTCGGGC‑AAAAAACCCCGacacgcct                                                                                                                                                                                                               >  1:574585/1‑232 (MQ=255)

                      atgatgATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGACAG‑CCACGCTTA‑TTTGC‑CCGTTTTTC‑CGTCTTTTCCGGC‑ACAAAACCTCTGACCACtt                                                                                                                                                                                                               >  1:1174652/1‑239 (MQ=255)

                        gatgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGACAACGTTGT‑TTAGT‑CGGTTTCTC‑GCCGTTCTCGCGG‑ACAAACCCTccacacacac                                                                                                                                                                                                               >  2:2233610/1‑229 (MQ=255)

                        gatgatATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGTTTT‑TTTGC‑GCGGTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAAACGcct                                                                                                                                                                                                               >  1:665305/1‑235 (MQ=255)

                              atCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCGA‑CCCCGTTTT‑TTTGCGGCGTTT‑TC‑CGCGATTTCCGGC‑CCAAACCCCCCAACCCCTTAC‑CTGCGt                                                                                                                                                                                                      >  1:1913254/1‑239 (MQ=255)

                              atCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGt                                                                                                                                                                                                      <  1:574573/239‑1 (MQ=255)

                                ccTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGTGCAG‑GCCCGTTTT‑TTTGC‑GCGGTTTTC‑GTCGCTTTCTGGC‑TAAAAACCTCGAAACACTTTA‑TGGCGttt                                                                                                                                                                                                    >  1:1760600/1‑239 (MQ=255)

                                     aCTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGG‑GCACGTTTT‑TTTGT‑GGGGTTCTC‑TGCCGTCTCGTGCGAAAAACCCCCG‑ACCTCTTTCTTGGCGtttttct                                                                                                                                                                                                >  2:824879/1‑236 (MQ=255)

                                     aCTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCt                                                                                                                                                                                               <  1:1586460/239‑1 (MQ=255)

                                         aaGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGTGGGAG‑CCACGGTTT‑TTTGC‑GCGTTTTTT‑CGCCTTTTCCGGC‑AAAAAACCCCCAACCCCCTAC‑TTGGGGTTTTGCtctgt                                                                                                                                                                                           >  1:930548/1‑235 (MQ=255)

                                          aGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAG‑CCACGGTTA‑TTTGG‑GCGTTTTTC‑GGCGTTTTCGGGC‑GAAAACCATCCAAACGCCTAC‑TGGCGGTTTTGCTTgggt                                                                                                                                                                                          >  1:1299600/1‑237 (MQ=255)

                                             tgcacttttGATATCCTCACAATTCGCTTATTCGTCGGAGTAAGAACGCGGATTACAATGTTGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGttt                                                                                                                                                                                         <  2:1378483/232‑1 (MQ=255)

                                                 atgttGATATCCTTAGAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCAGCAGTTATTTTGACGATCTGACAGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGAGCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAGCCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTgcggc                                                                                                                                                                                   <  2:830293/236‑1 (MQ=255)

                                                 aTTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTgcggc                                                                                                                                                                                   <  1:1556834/239‑1 (MQ=255)

                                                      gATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGa                                                                                                                                                                              <  1:824879/239‑1 (MQ=255)

                                                      gATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑GTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGa                                                                                                                                                                              <  1:1186859/239‑1 (MQ=255)

                                                         atCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCa                                                                                                                                                                           <  1:305870/239‑1 (MQ=255)

                                                           ccTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATg                                                                                                                                                                         <  1:1701224/239‑1 (MQ=255)

                                                            cTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑aaa                                                                                                                                                                                                                               <  1:1445241/185‑1 (MQ=255)

                                                                   tACGCTTGTTCGGCGGAGTAAGACCCCGGATAACGATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATATGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcat                                                                                                                                                                 <  2:1871490/239‑1 (MQ=255)

                                                                   tACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTGCCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcat                                                                                                                                                                 <  1:727967/239‑1 (MQ=255)

                                                                   tACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcat                                                                                                                                                                 <  1:1196281/239‑1 (MQ=255)

                                                                   tACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGctcat                                                                                                                                                                 <  1:2076275/239‑1 (MQ=255)

                                                                         tGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCAtt                                                                                                                                                           <  1:481961/239‑1 (MQ=255)

                                                                         tGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCAtt                                                                                                                                                           <  1:2044597/239‑1 (MQ=255)

                                                                                 ggAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGaa                                                                                                                                                    <  1:1724104/238‑1 (MQ=255)

                                                                                           cccGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGc                                                                                                                                         <  1:1711156/239‑1 (MQ=255)

                                                                                           cccGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGc                                                                                                                                         <  1:1511285/239‑1 (MQ=255)

                                                                                                 tAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACt                                                                                                                                   <  1:1353641/239‑1 (MQ=255)

                                                                                                   aCAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTACCCATACTGGCGGGGCCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAATCCCTCGAACCGCTTAC‑TGGCTTTTCCCCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTag                                                                                                                                 <  2:1503750/239‑1 (MQ=255)

                                                                                                           gatgatCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGaa                                                                                                                         <  2:939464/239‑1 (MQ=255)

                                                                                                                tcatcaGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTgcgg                                                                                                                                                                                    <  1:85506/175‑1 (MQ=255)

                                                                                                                tcatcaGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAAt                                                                                                                    <  1:228696/239‑1 (MQ=255)

                                                                                                                tcatcaGTTATTGTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTTCCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAAt                                                                                                                    <  1:1507758/239‑1 (MQ=255)

                                                                                                                        tATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCCCGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCATAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGc                                                                                                               <  1:1984167/236‑1 (MQ=255)

                                                                                                                        tATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGc                                                                                                               <  2:2176934/236‑1 (MQ=255)

                                                                                                                        tATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑GTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGc                                                                                                               <  1:1232470/236‑1 (MQ=255)

                                                                                                                        tATTTGGACGTGCTGCCTGAAGGTGAGGATTTATAAGGGGTTGTCGTTGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCAGGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTTTCGGGC‑AAAAGCCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTGTGCGGCGGGTATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCTCACTAGCAGCTGAAGGAATGTCGc                                                                                                               <  2:335307/236‑1 (MQ=255)

                                                                                                                          ttttTACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTTCCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCtgtg                                                                                                          <  1:1018362/239‑1 (MQ=255)

                                                                                                                          ttttGGCGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCtgtg                                                                                                          <  1:2233610/239‑1 (MQ=255)

                                                                                                                          ttttGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCtgtg                                                                                                          <  1:1842767/239‑1 (MQ=255)

                                                                                                                            ttGACTATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGtt                                                                                                        <  1:1602709/239‑1 (MQ=255)

                                                                                                                            ttGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCCG‑CCACGTTTA‑TTGGC‑TCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGtt                                                                                                        <  1:950450/239‑1 (MQ=255)

                                                                                                                            ttGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGtt                                                                                                        <  1:1296460/239‑1 (MQ=255)

                                                                                                                                      gCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGa                                                                                              <  1:995026/239‑1 (MQ=255)

                                                                                                                                      gCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGa                                                                                              <  1:697103/239‑1 (MQ=255)

                                                                                                                                      gCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGa                                                                                              <  2:685371/239‑1 (MQ=255)

                                                                                                                                           aaGGCGAAGATTTATAAGGTGTTGTCGATGTCAGTACCGCTCATTCTGCCCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                                                         <  1:2232926/239‑1 (MQ=255)

                                                                                                                                            aGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                                                         <  1:227937/238‑1 (MQ=255)

                                                                                                                                                     tttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCg                                                                                 <  1:1747907/237‑1 (MQ=255)

                                                                                                                                                      ttATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGa                                                                                                                                                                              <  1:496275/143‑1 (MQ=255)

                                                                                                                                                                 tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCG‑TTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtt                                                                  <  1:1317286/239‑1 (MQ=255)

                                                                                                                                                                       tGTCAGTGCCTCTCATTCTGCCCATACTGGCGGGGGCAG‑CCCCGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGc                                                             <  1:928156/239‑1 (MQ=255)

                                                                                                                                                                       tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGc                                                             <  1:1569406/239‑1 (MQ=255)

                                                                                                                                                                          cAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                                                           <  1:1825770/238‑1 (MQ=255)

                                                                                                                                                                             tACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTagcag                                                                                                                              <  1:485680/168‑1 (MQ=255)

                                                                                                                                                                             tACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGc                                                                                                               <  1:944747/183‑1 (MQ=255)

                                                                                                                                                                                   tcATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGTCTATTTTGGCCTGGACCGCATg                                                 <  1:474579/239‑1 (MQ=255)

                                                                                                                                                                                   gcATTCTGACCCTACTGGCGTGGGTGG‑CCCCGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTTTCGGTG‑AAAAACCCCCGAACCGCTTGC‑TGGGGTTTTGGCTAGTTTTTGCGGGGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCATGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATg                                                 <  2:1920877/238‑1 (MQ=255)

                                                                                                                                                                                           aCCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGaa                                                                                                                         <  1:1678689/159‑1 (MQ=255)

                                                                                                                                                                                           aCCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCt                                                           <  1:111533/221‑1 (MQ=255)

                                                                                                                                                                                                       gggggcgacgccGTTTC‑TTGGC‑TCGTTTCTC‑GGCGTTTCCGTGC‑CAGACCCCTCGAACCTCTACA‑TTGCTTTTTCCCTAGTTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAATTGCTTCCTGCCGCGCTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCTTGGACCGCATGTTGCCACATGCTCATCCg                               <  2:718280/227‑1 (MQ=255)

                                                                                                                                                                                                                    tttA‑TTGGC‑GCTTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTc                                    <  1:1599124/220‑1 (MQ=255)

                                                                                                                                                                                                                     ttA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGACGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCaa                >  1:1449556/1‑239 (MQ=255)

                                                                                                                                                                                                                          tGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAACCCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAAt            <  1:1288563/239‑1 (MQ=255)

                                                                                                                                                                                                                          tGGC‑GCGTTTCGC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCGGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAAt            <  1:641478/239‑1 (MQ=255)

                                                                                                                                                                                                                                     ctc‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTTGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGcc  <  1:288597/239‑1 (MQ=255)

TGCTGGCATACGTGTTGAAGACATGATGATATCCTTAACTAAAGGTGTCATTTTGATATCCTCACAATACGCTTGTTCGGCGGAGTAAGAACCCGGATAACAATGATGATGATCATCAGTTATTTTGACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAG‑CCACGTTTA‑TTGGC‑GCGTTTCTC‑GGCGTTCTCGGGC‑AAAAACCCTCGAACCGCTTAC‑TGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCC  >  NC_007779/3181040‑3181504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`