BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	295,781	A→T	8.4%	S180R (AGT→AGA)	Y75_RS01450 ←	integrase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	295,781	0	A	T	8.4%	825.7 / 12.2	288	S180R (AGT→AGA)	Y75_RS01450	integrase family protein
Reads supporting (aligned to +/- strand): ref base A (94/165); new base T (22/5); total (117/171)
Fisher's exact test for biased strand distribution p-value = 6.79e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

ACGTTGTAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAAC  >  NC_007779/295548‑296012

aCGTTGTAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTTAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGTGTGAGCAAACCAGACAATGGGCCCGACGCAGTC‑GGGGCATGGCCTCTTTTTTTAttttt                                                                                                                                                                                                                                      >  2:1992976/1‑238 (MQ=255)

aCGTTGTAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCACCGATAACGGCAGGGGGGACAAAATAACAAATGGGCCGGACACAAGC‑GGGCCTCGGGCACTTTTTTTACtttt                                                                                                                                                                                                                                      >  2:1075421/1‑238 (MQ=255)

     gTAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAACAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTc                                                                                                                                                                                                                                 <  2:910189/239‑1 (MQ=255)

      tAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAAACAGCCAATGGGCCAGCCGCAGCC‑GGGCCTTGGCCTCTTTTTTTACTTTTCCCTc                                                                                                                                                                                                                                 >  2:2106973/1‑238 (MQ=255)

            tgtgCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTTGGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCCTGGCCTCTTTTTTTACTCTTGCCTCCCCg                                                                                                                                                                                                                             >  1:2066789/1‑236 (MQ=255)

             gtgCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTg                                                                                                                                                                                                                         >  1:1792275/1‑239 (MQ=255)

              tgCCAGCTCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGGGAGCCAAACATACCATGGGTCAGCCGACGGT‑AGGCCTTGGTCCCTTTTTTTTATTTCTACTCCACAAcgta                                                                                                                                                                                                                        >  2:2015658/1‑236 (MQ=255)

              tgCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGATGCTATGTAATCTGGGGATAGCTCCGATAACGGCAGGGTTGGCCATCTAGCCACTCGATCGACCGAACTC‑GGGGGCTGGGCTCTTTGTTTTCTTCCACCCCCACGACCgg                                                                                                                                                                                                                        >  2:1348375/1‑239 (MQ=255)

              tgCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTGGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCCATGGTCCAGCCGAAGTC‑GGGCCTTGGGCTCTTTTTTTTCTTTCGCCTCCACGACTgg                                                                                                                                                                                                                        >  1:1420096/1‑239 (MQ=255)

              tgCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATCACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGGCTTGGCCTCTTTTTTTACTTTCGCCCCCACGACTgg                                                                                                                                                                                                                        >  1:1299030/1‑239 (MQ=255)

               gCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGa                                                                                                                                                                                                                       <  1:283372/239‑1 (MQ=255)

                       gCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGa                                                                                                                                                                                                                            <  1:1438516/226‑1 (MQ=255)

                             aaTGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAAtgtg                                                                                                                                                                                                           <  1:1538230/237‑1 (MQ=255)

                              aTGTTCACTTGAATTTTTTTAGGATACTTACACCA‑TTTGTTGAAAGCCCGTATGAGGCTATAAGCGTGAGCAGTGACGGTAGTTCTATTTTGCCTTTCTGT‑ACTCTGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGTCAGGTTGAGCAATCTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTTGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGAt                                                                                                                                                                                                         <  2:338278/238‑1 (MQ=255)

                              aTGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGAt                                                                                                                                                                                                         <  1:1654355/238‑1 (MQ=255)

                              aTGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGAt                                                                                                                                                                                                         <  1:1747957/238‑1 (MQ=255)

                                  tCCCTTGTAATTTTTTCGGTTAATTA‑TCCAGTTTGATGAAAGCACGTGATAGGCCATAGGCGGGTGCATTGACGGTAGGGCTATTTTGCCTTTCTGT‑ACTCGGCTACGCTCCTATGTAATTTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAGCTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCa                                                                                                                                                                                                    <  2:950794/239‑1 (MQ=255)

                                  tCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCCCGTAGTAGGCGATAAGCGTGAGCAGGGACGGTAGGTCTATTTTGCCTTTCGGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGc                                                                                                                                                                                                     <  1:72022/238‑1 (MQ=255)

                                      ttGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAACTATCCAATGGGCCAGCCGCAGGC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCCCGACTGGACAAGGTAATGTGATCGGCAATGt                                                                                                                                                                                                >  1:1500436/1‑239 (MQ=255)

                                       tggtatTTTTTCTTATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTTTTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTa                                                                                                                                                                                               <  2:271202/236‑1 (MQ=255)

                                       tGATATTTTTTTTGATAATTATTCCT‑GTTGATGAAAGCACGTGGTAGGCGATAGGCGTGAGCAGTGACGTTAGGTGTATTTGGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTa                                                                                                                                                                                               <  2:377231/239‑1 (MQ=255)

                                       tGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTTAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTa                                                                                                                                                                                               >  1:2111426/1‑239 (MQ=255)

                                       tGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCCAACTCGCCAATGTGCCAGCCGAAGAC‑GGGCCTTGCGCTCCTTTTTTACTTTCTCCTCCACGCCTCGGAAAGCTAATGTGATCGCCAATGTa                                                                                                                                                                                               >  2:928691/1‑239 (MQ=255)

                                       tGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGGGCAAAATAGCAAAAGGTCCAGCCGGAGTC‑GGGCCTTGGCCTCTTTTTTTATTTTTTCCTCCCCGACTGGACAAGGTAATGTGATCGGCCATgga                                                                                                                                                                                               >  2:476924/1‑237 (MQ=255)

                                            ttttttCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGTTTAGCTCCGATAACGGCAGTTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAg                                                                                                                                                                                          <  1:635413/239‑1 (MQ=255)

                                            ttttttCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAg                                                                                                                                                                                          <  1:1023860/239‑1 (MQ=255)

                                              ttttCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGTGCCAACTAGCCAATGGTCTAGCCGACGTC‑TGGCCTTGGGCCCTTTTTTTATTTTCTCCTCCACGCCTGGACAAGTTTAAGTGATCGGCACTGTATCGAgga                                                                                                                                                                                        >  2:1662816/1‑237 (MQ=255)

                                                               cA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATTGTCCAGCCGCAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCCCGACTGGGCCAGTTAATGTGAGCGGCAATGTATTGAGTTGAATATTGGCGTTTAGt                                                                                                                                                                       >  1:691268/1‑239 (MQ=255)

                                                                a‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑CCTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAg                                                                                                                                                                                          <  1:278778/219‑1 (MQ=255)

                                                                  tttGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAACTAACCAATGGTGCAGCCGGAGGCGGGGCCTGGGCCTTGTTTTTTA‑TTTCGCCCCCACGACCGGGACAGTTAATGTGATCGGCAATGTATCCAGTAGCATATTGGGTGTTAGttt                                                                                                                                                                     >  2:843913/1‑239 (MQ=255)

                                                                       tgaAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCCAACTAGCCAAAGGTCCAGCCGAAATC‑GGGCCTTGGCCTCTTTTTTTTCCTTCCCCTCCAAGAACGGGCAAGTTAATGTGGTCGGCCATGtt                                                                                                                                                                                               >  1:2222047/1‑207 (MQ=255)

                                                                       tgaAAGACCGTAGTAGGCGATAAGCGTGGGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGTTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCa                                                                                                                                                                <  2:1938117/239‑1 (MQ=255)

                                                                        gaAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCCAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTTGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGc                                                                                                                                                                 >  1:1772478/1‑237 (MQ=255)

                                                                             ctcGTATTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTGCTGT‑ACGCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCGGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACa                                                                                                                                                                                                                     <  1:2141651/178‑1 (MQ=255)

                                                                                gtagtaGGCGATAAGCGTGAGCAGTGACGGTAGTTCTATTTTGCCTTTCTGT‑ACGCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCg                                                                                                                                                      <  1:1966112/239‑1 (MQ=255)

                                                                                gtagtaGGCGATAAGCGTGAGCAGTGACGGTAGGACTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCGAACTAAACAATGGGCCAGCCGAAGTG‑GGGCCATGGCCTCCTTTTTTACTTTCGCCTCCACGACTGGACCAGTTCAt                                                                                                                                                                                                              >  1:5661/1‑184 (MQ=255)

                                                                                      ggCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCAAGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTTCTTTCGCCTCCACGACTGGACAAGTTAATGTGACCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACCG‑ATACCGGTtctc                                                                                                                                                >  1:1611340/1‑239 (MQ=255)

                                                                                       ggtaTAAGCGTGGGCGGTGACGTTGGGTCTACTTTGCCTTT‑TGTGATTCAGGCATCCTGTCATGTAATCTGGGGTTAGCTCTGTAAAGGGCTGGTTGAGATAACTACCGAATGGTCCAGCCGAAGTC‑GTGTCGTGGCCTCTTTTTGTACTTTCGCGTCCACTACTGGACAAGTTAATGTTATCGGCAATGTATCGTTTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATCCCGGTtctc                                                                                                                                                <  2:1779235/235‑1 (MQ=255)

                                                                                          aTAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGGGCAAAAAAGCCAATGGGCCAGCCGAAGTC‑GGGGCTTGGCCCCTTTTTTTACTTTCCCCCCCCCGAATGGACAAGGTAATGTGATCGGCAATGTATTGAGTAGAATATgg                                                                                                                                                                                >  1:227016/1‑204 (MQ=255)

                                                                                            aaGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTGGCGCAAACCAGCCAATGGTCCCGCCGAAGAC‑GGGCCCTGGGCTCTTTTTTTACTTTTGCCTCCCCGCCCGGTGAAGATAATGTGGTCGGGCATTTATCGTGTAGCAAATGGGCGTGTAGTTTTTGTACTGAATACCGCttacaatttt                                                                                                                                           >  2:290709/1‑231 (MQ=255)

                                                                                                        tGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAata                                                                                                                                                                                   >  1:192238/1‑187 (MQ=255)

                                                                                                        tGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGCAGTC‑GGGGCCTGGGCTCTTTTTTTAATTTCGCCTCCACGACTGGACCAGCTAATGTGCACGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGGTCTCAATTCTTTAAGATAGTCt                                                                                                                              >  1:120105/1‑239 (MQ=255)

                                                                                                         gACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGGTGAGCAAACTAGCCACTGGGTCAGCCGAAGTC‑GGGGCCTGGCCCCTTTTTTTTCTTTCGCCTCCCCGACTGGGAAAGTTAATGGGTTTGGGCATGTTTTGGGTGGAATATGGGCGGGTAGTTTTTGCACTG‑TAACCGGTTCTCAATTATTGTAGATAGTAtt                                                                                                                             >  2:104521/1‑239 (MQ=255)

                                                                                                                  tCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTGCGATAACGGCAGTTTGAGTAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCGTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGgaga                                                                                                                    <  2:1517410/239‑1 (MQ=255)

                                                                                                                    ttttttGCCTTTCTGT‑ACTCAGCCCGGCTGCTATGTAATTTGGGGTTAGCTCCGATAACGGAAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACg                                                                                                                  <  2:1772478/237‑1 (MQ=255)

                                                                                                                    tATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACg                                                                                                                  >  1:1001995/1‑239 (MQ=255)

                                                                                                                      ttttCCCTGTGTGT‑ACGCAGCCAGTCTGCTATGTAATCTGGGGTTAACTCGAAGAACGGAGCGTGGAACAACCTGGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGga                                                                                                                <  2:1902005/239‑1 (MQ=255)

                                                                                                                           ccTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGttt                                                                                                                                                                          <  2:344469/177‑1 (MQ=255)

                                                                                                                           ccTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAAATAGCCCATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGttt                                                                                                                                                                          >  1:344469/1‑177 (MQ=255)

                                                                                                                                       tCAGCCACGCGGTTATGTAATCTGGGGTTAGCTCCGATACCGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGtt                                                                                               <  2:934793/239‑1 (MQ=255)

                                                                                                                                         aGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGtttt                                                                                             >  1:1727598/1‑239 (MQ=255)

                                                                                                                                           ccACGCTGCTATGTAATCTGGGGTTAGCTCCGATATCGGCAGGGTGGGCAAACTAGCCAATGGTGCAGCCGGAGCC‑GGGCCTTGGCCTCTTTTTTTACTTTCCCCTCCACGACTGGACCAGGTAATGTGATCGGCCATGTATCGGGCAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGGTCTCAATTCTTGTAGATAGTCTTCCCAGGCGACGGAGAAAAGTC‑CTTTTTTTTTat                                                                                           >  2:497945/1‑238 (MQ=255)

                                                                                                                                              cGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTg                                                                                        >  1:610347/1‑239 (MQ=255)

                                                                                                                                                ctgctATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGACCAAAATAGCCAATGGGCCAGCCGCAGTC‑GGGCCTTGGCCTCTTTTTTTACCTTCACCTCCACCGCTGGACAAGTTAATGTGGTCTGCAATGTATCGTGTAGGATATGGGCGGTTAGTTTTTTAACTG‑ATAACGTGTCTCAATTATTGAAGATAGTCTTCCCCTGCGAGGGAG‑AAAGTCCCTTTTGtttttagttgt                                                                                      >  2:784824/1‑232 (MQ=255)

                                                                                                                                                   ctATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGAt                                                                                      >  1:827440/1‑236 (MQ=255)

                                                                                                                                                      tGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAg                                                                                                                                                                                          <  1:1589255/135‑1 (MQ=255)

                                                                                                                                                      tGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGAAATCTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCt                                                                                                                                          <  2:2192517/182‑1 (MQ=255)

                                                                                                                                                           tCTGGGGTTAGCTCCGATACCGGAAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGa                                                                                                                                                                                                                            <  2:433091/96‑1 (MQ=255)

                                                                                                                                                           tCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGtttt                                                                                                                                                                    >  1:1671025/1‑152 (MQ=255)

                                                                                                                                                           tCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAAATAGACAATGGTCCAGCCGCAGTC‑GGGGCTTTGGCTCTTTTTTTACTTTCGGCTCCACGCCTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATTGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGTAGATAGTCCTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTTCGTGATTCTGCCTGCAg                                                                           >  1:1173437/1‑239 (MQ=255)

                                                                                                                                                             tGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCa                                                                                                                                                                                                    <  2:2078752/118‑1 (MQ=255)

                                                                                                                                                             tGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAggg                                                                         >  1:98294/1‑239 (MQ=255)

                                                                                                                                                             tGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGGCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGGGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAggg                                                                         >  1:951495/1‑239 (MQ=255)

                                                                                                                                                                ggTTAGCTCCGATAACGGCAGGTTGGGGAAAATAGCCCATGGTCCAGCCGAAGTC‑GGGCCTTGGGCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGGGATCGGCGATGTATTGAGGAGAATATgg                                                                                                                                                                                >  2:1589255/1‑135 (MQ=255)

                                                                                                                                                                ggTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAtt                                                                      >  1:133382/1‑239 (MQ=255)

                                                                                                                                                                   tAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGAttct                                                                    >  1:630383/1‑238 (MQ=255)

                                                                                                                                                                    aGCTCCGATAACGGCAGGGTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTTCTTTTGCCTCCCCGACTGGACAAGTTAATGTGGTCGGCAATTTATTCAGTAGGATATGGGGGGTTAGTTTTTGCAATG‑ATACCGGGTCTTAATTCTTGAAGATAGTCTTCCCAGGCCAAGGTG‑AAAGTCCCTTTTTTTTTTAGCGATTCTGCCTTCAGGGGttct                                                                    >  2:628545/1‑237 (MQ=255)

                                                                                                                                                                     gCTCCGATAACGGCAGGGTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAgg                                                                                                                                                                                                                  >  1:433091/1‑95 (MQ=255)

                                                                                                                                                                            tAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGa                                                          <  2:2111426/239‑1 (MQ=255)

                                                                                                                                                                                  cgagtgtgccAAACTAGCCACTGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                     <  2:1611340/229‑1 (MQ=255)

                                                                                                                                                                                  cAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGAttttt                                                     <  2:1727598/238‑1 (MQ=255)

                                                                                                                                                                                   agtttGAGCAATCTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTa                                                    <  2:1136753/235‑1 (MQ=255)

                                                                                                                                                                                    ggTTTAGCAAACTAGCCAATGGGCCCACCGAAGTC‑TGGCCCTGGCCTCTTTTTTTTCTTTCGCCTCCACCACCGGGCAAGATAATGTGATCGGCAATGTATCGAGTAGCATCTGGGCGTTTAGTTTTTTCACTG‑ATACCGGTTCTCCATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACa                                                  >  2:507504/1‑239 (MQ=255)

                                                                                                                                                                                    ggTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTAc                                                   <  2:2175921/238‑1 (MQ=255)

                                                                                                                                                                                         aGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATAc                                                                                                                                                        >  1:1387355/1‑133 (MQ=255)

                                                                                                                                                                                          gCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTa                                            >  1:1848237/1‑239 (MQ=255)

                                                                                                                                                                                                  cccAATGGTCCAGCCGAAGTC‑GGGCCTGGGCCTCTTTTTGTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTGTACAGCCTTAGCAATTCg                                    <  2:1173437/238‑1 (MQ=255)

                                                                                                                                                                                                    cAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                   <  1:2092615/238‑1 (MQ=255)

                                                                                                                                                                                                    cAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                   <  1:2177994/238‑1 (MQ=255)

                                                                                                                                                                                                    cAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                   <  1:476924/238‑1 (MQ=255)

                                                                                                                                                                                                    cAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                   <  1:843913/238‑1 (MQ=255)

                                                                                                                                                                                                    cAATGCTCCCGCCGAAGTC‑GGGCCTTGGCCTCTTGTTTTACTTTCGACTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGTAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGt                                   <  1:1071616/238‑1 (MQ=255)

                                                                                                                                                                                                       tGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGa                                >  2:706128/1‑238 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTGGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:1713118/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  1:2020004/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:1001995/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  1:882627/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:827440/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:1420096/239‑1 (MQ=255)

                                                                                                                                                                                                        ggTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:610347/239‑1 (MQ=255)

                                                                                                                                                                                                         gTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:1843200/238‑1 (MQ=255)

                                                                                                                                                                                                         gTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATc                              <  2:2234150/238‑1 (MQ=255)

                                                                                                                                                                                                            cAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTa                                                    <  1:503814/213‑1 (MQ=255)

                                                                                                                                                                                                                   aGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTAt                   <  1:784824/239‑1 (MQ=255)

                                                                                                                                                                                                                    gTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGAt                               >  1:1250717/1‑226 (MQ=255)

                                                                                                                                                                                                                    gTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGAt                               <  2:1250717/226‑1 (MQ=255)

                                                                                                                                                                                                                         ggCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATc              >  2:2040831/1‑239 (MQ=255)

                                                                                                                                                                                                                          gCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCCATTCGTGGATCTATCCCTGTATCGATCa             >  2:1147587/1‑239 (MQ=255)

                                                                                                                                                                                                                           catttgCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATTCCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCa             <  2:1388595/233‑1 (MQ=255)

                                                                                                                                                                                                                                    ctTTTTTTTCTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTGTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCGGCGATTTTTACAGCCTTAGCAATTCGTGGATCTACCCCTGTATCGATCAATGTTTGTaa   <  1:131082/239‑1 (MQ=255)

ACGTTGTAATCTTGTGCCAGATCGCCAGGAATGATCCCTTGATATTTTTTCTGATAATTACTCCA‑TTTGATGAAAGCACGTAGTAGGCGATAAGCGTGAGCAGTGACGGTAGGTCTATTTTGCCTTTCTGT‑ACTCAGCCACGCTGCTATGTAATCTGGGGTTAGCTCCGATAACGGCAGGTTGAGCAAACTAGCCAATGGTCCAGCCGAAGTC‑GGGCCTTGGCCTCTTTTTTTACTTTCGCCTCCACGACTGGACAAGTTAATGTGATCGGCAATGTATCGAGTAGAATATGGGCGTTTAGTTTTTGCACTG‑ATACCGGTTCTCAATTCTTGAAGATAGTCTTCCCAGGCGACGGAG‑AAAGTCACTTTTGTTTTACGTGATTCTGCCTGCAGGGATTCTGCTTCTGCGATTTTTACAGCCTTAGCAATTCGTGGATCTATCCCTGTATCGATCAATGTTTGTAAC  >  NC_007779/295548‑296012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`