BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	3,941,168	T→C	8.2%	E345G (GAA→GGA)	bcsG ←	cellulose biosynthesis protein BcsG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	3,941,168	0	T	C	8.2%	847.5 / 7.0	307	E345G (GAA→GGA)	bcsG	cellulose biosynthesis protein BcsG
Reads supporting (aligned to +/- strand): ref base T (189/81); new base C (2/30); total (191/116)
Fisher's exact test for biased strand distribution p-value = 1.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

AAGGCGTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGCGGAGTTAAAGTTCTTGAACTCAAT  >  NC_007779/3940931‑3941406

aagGCGTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑c                                                                                                                                                                                                                                                >  1:59672/1‑239 (MQ=255)

aagGCGTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑c                                                                                                                                                                                                                                                >  1:1774485/1‑239 (MQ=255)

 agGCGTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑Cg                                                                                                                                                                                                                                               <  2:1669550/239‑1 (MQ=255)

     gTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGc                                                                                                                                                                                                                                              >  1:1590828/1‑236 (MQ=255)

     gTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAACCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGc                                                                                                                                                                                                                                              >  1:85406/1‑236 (MQ=255)

       ccAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCAACAA‑T‑T‑CGCCCTg                                                                                                                                                                                                                                          >  2:1454786/1‑238 (MQ=255)

          gTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCAGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTGCGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CACTCTGCAt                                                                                                                                                                                                                                       >  1:1408172/1‑238 (MQ=255)

                caAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCCTCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑C‑CGCCCAGCATgcccc                                                                                                                                                                                                                                  >  2:1617855/1‑235 (MQ=255)

                      ttttCTGCGCCCGCGCTTTGTAATCCGCTGTATTGCTGACCCTCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTTCGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCTGTTAAGCACGGTGGTATGGT‑CATAAACCGACATGCGTACAAGGCCCAGCAAAGTAACCGGCAG‑AGCTGTTTGATCCTTCAT‑T‑T‑AGATCTGAATGCGGCC‑Attgccg                                                                                                                                                                                                                         <  2:1008429/238‑5 (MQ=255)

                          cTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGaa                                                                                                                                                                                                                     <  1:661797/239‑1 (MQ=255)

                               cccGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑Cg                                                                                                                                                                                                                                               <  1:351063/209‑1 (MQ=255)

                               cccGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAActtct                                                                                                                                                                                                                >  1:1874500/1‑239 (MQ=255)

                                 cgcgCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTTTTTGATCAATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCttt                                                                                                                                                                                                              >  2:643847/1‑239 (MQ=255)

                                        tGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCaaaaaa                                                                                                                                                                                                       <  1:386952/239‑1 (MQ=255)

                                              ccGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAAccgccg                                                                                                                                                                                                 >  1:717390/1‑239 (MQ=255)

                                              ccGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAAccgccg                                                                                                                                                                                                 >  1:1450735/1‑239 (MQ=255)

                                                gCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑A‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGaa                                                                                                                                                                                               >  2:1570244/1‑239 (MQ=255)

                                                  tGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AATGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGa                                                                                                                                                                                                >  1:1046756/1‑236 (MQ=255)

                                                  tGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGTGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCCGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CCCTCTGCATGCCGCC‑CTTTTCGCGAACTTTTTTCCAAAAAacgcctcac                                                                                                                                                                                              >  2:1940170/1‑234 (MQ=255)

                                                  tGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑CTTTTCGCGAACTTCTTTCAAAAAACCGCCGa                                                                                                                                                                                                >  2:152497/1‑236 (MQ=255)

                                                  tGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCCCC‑CTTTTCGCGAACTTCTTTCCAAAAAccgccgc                                                                                                                                                                                                >  2:2088159/1‑235 (MQ=255)

                                                      ttGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGGTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATTCATCAC‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCCAAAAACCGCCCCACTGGcc                                                                                                                                                                                         >  2:220250/1‑239 (MQ=255)

                                                         cTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGtt                                                                                                                                                                                      >  1:1841376/1‑239 (MQ=255)

                                                          gggaccccGGATAAAGGGTGCCGTCCATCCAGT‑GAGGCGGGTTTGTACACGGTGGCA‑CTCCTGTTTTTTTTATTTTTTTCGTT‑ACCCCCCACCCACGGGTAAAGCACAGCGGTTTCGT‑AATAAACCGGCGAACCATCAAAGCCCACCAAAATACCCGGCAG‑ATTTTTTTGATCCA‑CAA‑T‑TCCTCTCTGCTTGCCGCC‑ATTTCCGCGACCTTCTTTCAAAAAACCGCCGAACTGGCCGtt                                                                                                                                                                                      <  2:900740/235‑1 (MQ=255)

                                                           gACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGaa                                                                                                                                                                                               >  2:506663/1‑228 (MQ=255)

                                                            aCCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATg                                                                                                                                                                                   >  1:769541/1‑239 (MQ=255)

                                                              ccccGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGt                                                                                                                                                                                  >  1:1614800/1‑238 (MQ=255)

                                                                ccGGGTAGTGGTTGCGGT‑TTTGGAGGGGAGGGGTG‑TTGGAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTCCGGT‑AAGGACAAGCCGGCGTTTAAGCACAGGGGTATCGT‑CATAAACCGGCAAACCATCAAAGCCCAGAAAAAAACCGGGCAG‑ATTTGTTTGATCAACCAA‑T‑C‑CGCTCTGCATGCCGCC‑ATTTCCGCGAACTTCTTTCAAAAATCCGCCGAACTGGCCGTTATGTccc                                                                                                                                                                               <  2:477706/239‑1 (MQ=255)

                                                                      aaTGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTGTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAACCCGGCGACCCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAACCCGCCGAACTGGCCGTTATGTCCCatcatc                                                                                                                                                                         <  1:504366/239‑1 (MQ=255)

                                                                        tGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATATCCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAg                                                                                                                                                                       >  1:919605/1‑239 (MQ=255)

                                                                           ttGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGt                                                                                                                                                                     >  1:27718/1‑238 (MQ=255)

                                                                             gCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑TATAAACCGGCGAACCATACAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCCCTGCATGCCGCC‑ATTTTCGCGAACTTCGTTCAAAAAACCGCCGAACTGGCCGGTATTTCCCCTCATCAGGTGcg                                                                                                                                                                  >  2:1758790/1‑238 (MQ=255)

                                                                             gCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGGTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGGATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTGTGAGCCATCAA‑T‑T‑TGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAAACGCCGAACTGGCCGGTATGTCCCATCATCAGGGGcg                                                                                                                                                                  >  2:2055254/1‑238 (MQ=255)

                                                                              ccGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTTCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACt                                                                                                                                                                                             >  2:215775/1‑211 (MQ=255)

                                                                                   cATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGTGGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAACCCGGCCAACCATCAAAGCCCAGCAAAATACCCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCCCGTCAGGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTa                                                                                                                                                             <  1:1940170/239‑1 (MQ=255)

                                                                                    aTGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTCTCTTTTTCGGT‑AACGTCCAGCCAGCGCTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAACATAACCGGCAG‑ATTTGTTTGATCCCTCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTaa                                                                                                                                                            >  1:534656/1‑239 (MQ=255)

                                                                                    aTGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTaa                                                                                                                                                            >  2:612007/1‑239 (MQ=255)

                                                                                          tGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATTTCCCCTCATCCGGTGCTGGGTAAAACCCa                                                                                                                                                      >  2:1206185/1‑239 (MQ=255)

                                                                                          tGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCa                                                                                                                                                      >  1:1229649/1‑239 (MQ=255)

                                                                                                  tg‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaa                                                                                                                                              >  1:1882990/1‑239 (MQ=255)

                                                                                                  tg‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaa                                                                                                                                              >  1:1384299/1‑239 (MQ=255)

                                                                                                  tg‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGGTTCGaa                                                                                                                                              >  1:567436/1‑239 (MQ=255)

                                                                                                  tg‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTCTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaa                                                                                                                                              >  2:528677/1‑239 (MQ=255)

                                                                                                   g‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGACCCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaaa                                                                                                                                             <  1:30676/239‑1 (MQ=255)

                                                                                                   g‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaaa                                                                                                                                             <  1:612007/239‑1 (MQ=255)

                                                                                                   g‑ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaaa                                                                                                                                             <  1:1658072/239‑1 (MQ=255)

                                                                                                     ttgtAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCCTCATCCGGTGCTGGGTAAAGCCCAGTTTCGAAAg                                                                                                                                            >  1:380495/1‑239 (MQ=255)

                                                                                                      ttgaGAACGTGGCATCACCGGGTTTGTTAATTTTTTCCGAA‑ACCGCCCGGCCAGCGTTAAAGCCAAGC‑GTATTGTACATAACCGGGCGACCCATCAACGCCCAGCAAAATACCCGGGAG‑ATTTTTTGGACCAACAAA‑T‑C‑CGCTCTCCATGCCGCC‑ATTTTCCCGAACTTCTTCAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGaaa                                                                                                                                             <  2:388031/235‑1 (MQ=255)

                                                                                                           agcGTGGCA‑CTACGGCTTTTTTTATCTTTTTCGGA‑AACCTCAACCAAGCGTTTAAGCACAGCGGTTTCGT‑CATAACCCGCCGAACCTTCAAAGCCCAGAAAAATAACCGGCAG‑ATTTGTTTTATCCATCAA‑T‑T‑CGCTCTGCTTGCCGCC‑TTTTCCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTAt                                                                                                                                       <  2:27718/236‑1 (MQ=255)

                                                                                                            tggggccA‑CTAGGGCTGTTTTCGTTTGTTTCGTA‑AACGCCCGCCCTGCGTTTAGGAACAGGGTTTTGTT‑GATGACCGGGGGACCTATTAAACCCAAGCAAAATACCCGCGTG‑GTTTTTTGGACCCATGAG‑T‑T‑GCCTCTCACGGCCCCC‑ATTTTCCGGAACTTCTTTAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATc                                                                                                                                      <  2:320507/232‑1 (MQ=255)

                                                                                                            aCGGGGCA‑CTACGGTTGTTTTTATCTTTTCCGGA‑AACGCCCAGCCAGCGGTAAAGCACAGCGGTATCGT‑CATAACCCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑C‑CGCTCTGCATGCCGCC‑ATTTTCGCGACCTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATc                                                                                                                                      <  1:1334767/238‑1 (MQ=255)

                                                                                                             cGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGc                                                                                                                                                                   >  2:1867507/1‑208 (MQ=255)

                                                                                                             cGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGCTATCaa                                                                                                                                    >  1:1921185/1‑239 (MQ=255)

                                                                                                              gTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCaaa                                                                                                                                   <  1:65757/239‑1 (MQ=255)

                                                                                                                ggCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCg                                                                                                                                                                                        <  1:1599697/184‑1 (MQ=255)

                                                                                                                ggCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACa                                                                                                                                 >  1:1977871/1‑239 (MQ=255)

                                                                                                                ggCA‑CTACGGCTGTTTTTATCTTTTTCGGG‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACa                                                                                                                                 >  1:2019454/1‑239 (MQ=255)

                                                                                                                        cGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCaaa                                                                                                                                   <  1:425296/230‑1 (MQ=255)

                                                                                                                        cGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGGATCGT‑CATAAAACGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGATATCAAACAGATAGCa                                                                                                                          >  1:1496637/1‑239 (MQ=255)

                                                                                                                          tcTGTTTTTATTTTTTGCGGA‑AACGCCCAGCCAGGGGTAAAGAAAAGGGGTCTCGT‑AAAAACCCGGCGACCCTACAAACCCCAGCAAAA‑AACCCGTAGTATTTTTTTTTTCCACAAA‑T‑C‑CGCTCGGC‑TGCCCCCTATTTTCGCAACCTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAg                                                                                                                         <  2:420061/237‑1 (MQ=255)

                                                                                                                                 ttATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAACCCGGCGACCCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATAAA‑T‑G‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTAtt                                                                                                                 <  1:22910/239‑1 (MQ=255)

                                                                                                                                   ttCTTTTTCGGT‑ACCGCCACCCCAGCGTTTAAGCACAGCGGTATCTT‑CATAACCCGGCAAACCATCAAAGCCCAGCAAAATACCGGGCAG‑ATTTTTTTGATCCATCAA‑T‑T‑CGCTCTGCGTCCCGCC‑ATTTTCGCGAACTTTTTTAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTg                                                                                                               <  2:1593594/238‑1 (MQ=255)

                                                                                                                                       ttttCGGTAAACGCCCCGCCCGCGGTTAAGCAAAGGTGTAGCTA‑CATAACCGCGCGACACATCAAGCCCCGACAAAAAAACCGGCAG‑ATTTTTTTGACCCAAAAA‑T‑C‑CGCTCTCCATGCCGCA‑TTTTCCGCGAACTTCTTTAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCg                                                                                                            <  2:143547/239‑1 (MQ=255)

                                                                                                                                        tttCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGt                                                                                                          <  1:25273/239‑1 (MQ=255)

                                                                                                                                        tttCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGt                                                                                                          <  1:1439632/239‑1 (MQ=255)

                                                                                                                                                    tCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGAtt                                                                                               <  1:474245/239‑1 (MQ=255)

                                                                                                                                                     ccgcacggcGGTAAACCAAAGCGGTATTCT‑CAAAACCCGGCAAACAACAAAACCCCAGCAAAATTCCCTGCAG‑TTTTGTTTGCACCATAAA‑T‑C‑CGCTCTGCATGCCGCA‑TTTTTCGCGAATTGTTTCCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTa                                                                                              <  2:919605/232‑1 (MQ=255)

                                                                                                                                                          caggGGTTAAGCCAAGCGGTATCGT‑CATAACCCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCAAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGtgtg                                                                                         <  1:1573645/239‑1 (MQ=255)

                                                                                                                                                                ttAAGCAAAGCGGTATCGT‑CATAACCCGGCAACCCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTTTTTGATCCATCAT‑T‑C‑CGCTCTGCATGCCGCC‑TTTTTCGCGAATTTTTTTAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTa                                                                                                                                                             <  2:203862/165‑1 (MQ=255)

                                                                                                                                                                       caGCGGTAGCGA‑CATAAACCGGCGAACCATCCAACCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCCTCCA‑T‑T‑TGCACCGCATGCCGCC‑ACTTTCACGAACTTCTTTCAAAAAACCGCCGAACCAGCCGCTATGCCCCCACATCAGGCCCTGGGCAAAACCCCGTTTCGAAAGGTTATCCAAACGATAACAGCCCTTGATTGCCGGGTGCCACACGATAGGGGTCGAAGTCTGccc                                                                             >  2:594306/1‑238 (MQ=255)

                                                                                                                                                                         gCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTCCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGtgtg                                                                                         >  1:828798/1‑224 (MQ=255)

                                                                                                                                                                         gCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTAGCGAATTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATTAAGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTAACAAACAGATAGCAATAGATATTTGCCGGTTGTTCCAGATTAGTGTGCGAAGCCTGCCCGCa                                                                          >  2:1183972/1‑239 (MQ=255)

                                                                                                                                                                          cGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGACCTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGt                                                                                   >  1:1476348/1‑229 (MQ=255)

                                                                                                                                                                          cGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCa                                                                          >  1:1513091/1‑238 (MQ=255)

                                                                                                                                                                          cGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCa                                                                          >  2:852746/1‑238 (MQ=255)

                                                                                                                                                                                  t‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGgcgc                                                                  >  1:1317420/1‑238 (MQ=255)

                                                                                                                                                                                     aTAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCa                                                               >  1:203991/1‑239 (MQ=255)

                                                                                                                                                                                        aaCCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTa                                                            >  1:2224848/1‑239 (MQ=255)

                                                                                                                                                                                             gCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAg                                                                                                                                                                       >  1:1100147/1‑127 (MQ=255)

                                                                                                                                                                                             gCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACgg                                                       >  1:216289/1‑239 (MQ=255)

                                                                                                                                                                                             gCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACgg                                                       >  1:1535910/1‑239 (MQ=255)

                                                                                                                                                                                              cGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGa                                                      >  1:100082/1‑239 (MQ=255)

                                                                                                                                                                                                     ccaacacccACCAAAAACACCGGCGT‑TTTTTTTTGACACATCAT‑T‑C‑CCCTCGACAGCCCGCA‑TTTTCCCCGAATTTTTTTAAAAAACCCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATcgccgc                                               <  2:1263378/233‑1 (MQ=255)

                                                                                                                                                                                                       aaaGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGccgccg                                             >  1:1263378/1‑239 (MQ=255)

                                                                                                                                                                                                         acccaaaaaaaaaaACCGCCA‑‑ATTTTTTTTAACCATTAACT‑T‑CGCTCTCCCTCCCCCC‑TTTTTCGCAATCTT‑TTTAAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGc                                          <  2:761801/231‑1 (MQ=255)

                                                                                                                                                                                                          cccaaGCAAAAAAACCGACAG‑ATTTGTTT‑AACCACCAA‑TCT‑GGTCCTCCATCCCGCT‑ATTTCCCAGATTTTCTTACAAAAACCCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGc                                          <  2:1977871/235‑1 (MQ=255)

                                                                                                                                                                                                           cccAGCAAAATACCGGGAAT‑ATTTTTTTGATCCAGCAA‑G‑T‑CGCTCTGCATGCCGCC‑TTTTTGCCGACCTTCTTTAAAAAAGCCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGcc                                         <  1:11920/239‑1 (MQ=255)

                                                                                                                                                                                                           ccagacaaagaaaCAGGCAG‑TTTGGTTGGATCAATACA‑T‑G‑CGCTCGGCTGCCCGCC‑TTTTTCGCGAATTTTTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTAGATGCAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGc                                          <  2:132996/227‑1 (MQ=255)

                                                                                                                                                                                                            cctgaaaaaTCCCCGGCAG‑TTTTGTTTGACCCATCAA‑T‑T‑CGCCGTGCGGCCCCCC‑ATTTCCGCGAACTTTTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCa                                        <  1:2088159/234‑1 (MQ=255)

                                                                                                                                                                                                              aGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGa                                                      >  1:811900/1‑223 (MQ=255)

                                                                                                                                                                                                              aGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGcc                                         >  1:1166141/1‑236 (MQ=255)

                                                                                                                                                                                                               gCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGGTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCCCtt                                     >  2:177981/1‑238 (MQ=255)

                                                                                                                                                                                                                          ggaaG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCCGTCGTTATTTGCCGGTTGTTACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCTCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGc                          >  1:1741360/4‑239 (MQ=255)

                                                                                                                                                                                                                          ggCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCcatcat                                                                                                                                                                          >  1:399093/1‑95 (MQ=255)

                                                                                                                                                                                                                                aTTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCGGGGCCACTGTAGGAGGTGGCGGAGt                     >  1:1147312/1‑239 (MQ=255)

                                                                                                                                                                                                                                 tttgtttgATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGGAGGTGGGGGCggtgct                    >  2:382716/1‑235 (MQ=255)

                                                                                                                                                                                                                                          tCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACgg                                                       >  1:521834/1‑195 (MQ=255)

                                                                                                                                                                                                                                          tCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGCGGAGTTAAAGTTCtt           >  2:793146/1‑239 (MQ=255)

                                                                                                                                                                                                                                          tCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGCGGAGTTAAAGTTCtt           >  1:881301/1‑239 (MQ=255)

                                                                                                                                                                                                                                             aTCAT‑T‑C‑CGCTCTGCTTGCGCCT‑ATTTCCGCGACTTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGCGGAGTTAAAGTTCTTGaa        <  1:220250/239‑1 (MQ=255)

AAGGCGTCCAGTTCATCAAAGAATTTCTGCGCCCGCGCTTTGTAATCCGCTGTTTTGCTGACCCCCGGATAATGGTTGCCGT‑CATGCAGTGGAAGCGTG‑TTGTAGAACGTGGCA‑CTACGGCTGTTTTTATCTTTTTCGGT‑AACGTCCAGCCAGCGGTTAAGCACAGCGGTATCGT‑CATAAACCGGCGAACCATCAAAGCCCAGCAAAATAACCGGCAG‑ATTTGTTTGATCCATCAA‑T‑T‑CGCTCTGCATGCCGCC‑ATTTTCGCGAACTTCTTTCAAAAAACCGCCGAACTGGCCGTTATGTCCCATCATCAGGTGCTGGGTAAAGCCCAGTTTCGAAAGGTTATCAAACAGATAGCAGTCGTTATTTGCCGGTTGATACAGATTAGTGTGCGAAGTCTGCCCGCAGCTGGCGCGCAGTAAACGGATCGCCGCCGGGCCACTGTAGGAGGTGGCGGAGTTAAAGTTCTTGAACTCAAT  >  NC_007779/3940931‑3941406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`