Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 4,104,271 T→C 18.1% L147P (CTC→CCC)  ompR → two‑component system response regulator OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,104,2710TC18.1% 213.9 / 12.0 126L147P (CTC→CCC) ompRtwo‑component system response regulator OmpR
Reads supporting (aligned to +/- strand):  ref base T (53/42);  new base C (22/3);  total (77/49)
Fisher's exact test for biased strand distribution p-value = 2.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCGTC  >  NC_007779/4104039‑4104506
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
cTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTa                                                                                                                                                                                                                                             <  1:493142/238‑1 (MQ=255)
cTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTa                                                                                                                                                                                                                                             <  2:1142831/238‑1 (MQ=255)
           gagCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAACGGCGGGAATTT‑ATTTTGGTAAGTTCCAACTTAAACC‑CGGTAAGCGCGcaagtt                                                                                                                                                                                                                                 >  2:1047082/1‑236 (MQ=255)
            agCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAAATT‑GTTTCGGGAAGGTCAAACTTAAACT‑CTGGAAGCGCCAAAAGtt                                                                                                                                                                                                                                >  1:1636501/1‑239 (MQ=255)
              cAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTC‑CTTTCGGGAAGTTAAAACTTAAACG‑CGGGAGGCGCGGAATggtac                                                                                                                                                                                                                              >  1:2153220/1‑235 (MQ=255)
                    gATGCCGATCATTATGGTGAGGGCGAAAGGGGAAGAGGTGGACCGTGTCGTGGGCCTGGAGAGTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTA‑AACGGCTGGCCCGTATCCGTGGGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCCCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGAAAGTTCAGACTTAACCT‑CGGTCCGCGCGAAATGTCCC‑GGGAAg                                                                                                                                                                                                                       <  2:542933/239‑1 (MQ=255)
                        ccGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGtt                                                                                                                                                                                                                                <  1:1987366/227‑1 (MQ=255)
                            tCATTATGGTCACGGCGAAAGGGGAAAAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGCGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGCAACTGCCTTTGGGTAGTTTCAACGTTACCTC‑CGGAAGCCGCAAAATGTTCC‑ACGAAGAccacac                                                                                                                                                                                                                >  2:795713/1‑234 (MQ=255)
                                       aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACCGGAAGAGGCGGTAACTG‑CTCTCGGTAAGATCAAAATTAACCC‑CGGGAAGCGCGAAACGTGCC‑GCGAGGAC‑GAGCCgcagcccgcca                                                                                                                                                                                                    >  2:722086/1‑228 (MQ=255)
                                       aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑CTTTCGGGAAATGCAAACTTAACAC‑CCGTACCGCCGAAATGGTCC‑GCGGAGAA‑GCGCCGaatccccccc                                                                                                                                                                                                    >  2:303309/1‑229 (MQ=255)
                                       aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑CGTTCGGGAAGTTTAAACCTAACCC‑CGGGCAGCGCGAAATGTTCC‑GGGGAAGC‑GAGCCggatccgcccc                                                                                                                                                                                                    >  2:1274458/1‑228 (MQ=255)
                                       aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGGAAAC‑GAGCCGATGCAGCTCa                                                                                                                                                                                                    >  1:1543541/1‑238 (MQ=255)
                                       aCGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAAATG‑CTTTCGGTAAGTTCAAACTTAACCC‑CGGGACGCGCGAAATGTTCC‑GCGCAGAC‑GAGGCCATGCCGCTCa                                                                                                                                                                                                    >  1:1061339/1‑238 (MQ=255)
                                                          tGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAAACT‑CGGTACGCGCGAAATGTTCC‑GCGGAGAC‑GAGCCGATGCCGCCCACCCGCGGTGAGGTTGC‑ggg                                                                                                                                                                                >  1:602260/1‑237 (MQ=255)
                                                           ggcccGTTTCGTAGGCCTGGAGATGGGCGCTGACGACTACATTCCAAACCCGTTTACCCCGCGTG‑AACGGCTGGCCCGTATCCGGGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGt                                                                                                                                                                                <  1:1428397/234‑1 (MQ=255)
                                                           ggACCGTATCGTAGGCCTGGAGATTGGCGCTGCCGACTACATTCCAAAACCGTTTACCCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTa                                                                                                                                                                               <  1:1274458/238‑1 (MQ=255)
                                                           ggACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTccccc                                                                                                                                                                                                 >  1:218498/1‑220 (MQ=255)
                                                            gACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CCGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCCCACCAGCGGTGAGTTTGC‑GGt                                                                                                                                                                                >  1:629494/1‑236 (MQ=255)
                                                            gACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGGAAGTG‑CGTTCGGGAAGGTCCAACTTAACCT‑CGGGACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCACACCAGCGGGGAGTTTGC‑GGt                                                                                                                                                                                >  1:582263/1‑236 (MQ=255)
                                                                 tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑CTTTCGGGAAGGTCCAACTTAACCC‑CGGGAAGCGCGAAATGTTCC‑GCGGAGAC‑CAGACGCACTCCCCCACCCCCGGGGAGTTTGCGGGAACTG‑AAg                                                                                                                                                                        >  1:34877/1‑239 (MQ=255)
                                                                 tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGGTTCAACTTAAACT‑CGGGACGCGCGAAATGTTCC‑GCGAAAAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGT‑GGTACttgagg                                                                                                                                                                        >  1:1309037/1‑234 (MQ=255)
                                                                 tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGGTCAAACTTAACCC‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCCCACCAGCGGGGGGTTTGC‑GGGACTG‑AAgg                                                                                                                                                                       >  1:1254825/1‑239 (MQ=255)
                                                                 tATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAACTCCAAATTAACCT‑CCGTACGCGCCGAACGTTTC‑GCGCAGAG‑GCGCCGGTGCCGGCCCCCAGCGGGGAGGTTGC‑GGtggcgggag                                                                                                                                                                        >  2:955590/1‑231 (MQ=255)
                                                                    cGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTTC‑GCGAAGAC‑GAGCCGATGCCGCCCACCAACGGGGAGGTTGC‑GGTACTG‑AAGGCa                                                                                                                                                                     >  1:1321815/1‑238 (MQ=255)
                                                                      tAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑CTTTCGGTAAGGTCAAACTTAACCT‑CCGGACGCGCGAAAAGGTCC‑GCGGAGAA‑GAAGCGGAGCCGCTCACCAGCGGGGCGGTTGC‑GGGAATGAAAGGCAcc                                                                                                                                                                   >  1:1971777/1‑238 (MQ=255)
                                                                         gCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGGAAGTTTAAACTTAACCC‑CGGTACGGGCGCAAAGTTCC‑CCGAAAAA‑GAGACGGAGCCGCTCACCCGCGGGGTGTTTGC‑GGTACTG‑aaagaccgtgg                                                                                                                                                                >  2:1119816/1‑229 (MQ=255)
                                                                              gagaTTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGGGATTG‑CGTTCGGGAAGTTCAAACTTAAACC‑CCGTACGCGCGCAATGTTCT‑GCGAAGAC‑GAGCCGATACCCCCCACCCGCGGGGTGGTTTA‑GGTGCTC‑AAGGCAC‑AGGTCAGcc                                                                                                                                                          >  2:328522/1‑238 (MQ=255)
                                                                              gagaTTGGCGCTGACGACTACATTCCAAAACCGTTCAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CCTTCGGTAAGTTCAAACTTAACCT‑CGGGACGCGCGAAAAGTTCC‑CCGCAAAA‑GAGCCGATGCCCCCCCCCCGCGGGGGGTTTGC‑GGGACTG‑GAAGCAC‑TGGGCAgac                                                                                                                                                          >  2:519035/1‑236 (MQ=255)
                                                                               agagTGGCGCTTACAGCTACCTTCCAACCCCTTTTAGCCGTGGTG‑AACTGGTGGCGCGTCTCCGGGGGGTGCGGTGTCGGCGGGGGAACGAACTGCCGGCGGGCCCGTCACGGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTACAACCTTAACCT‑CGGTACGCGCGAAATGGTCG‑GCGAAGCC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCa                                                                                                                                                         <  2:1437946/238‑1 (MQ=255)
                                                                                   tGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAA‑GAGCCGATGCCGCCCACCAGCGGGGAGGTTGC‑GGTACTG‑AAGGCAC‑TGGTGAGCCATCcgc                                                                                                                                                    >  1:1255530/1‑239 (MQ=255)
                                                                                   tGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAAAGTTCC‑GCGCAGAA‑GCGCCGATGCCGCTCACCCGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCAAccc                                                                                                                                                     >  1:1562866/1‑237 (MQ=255)
                                                                                   tGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGCCATTG‑CCTTCGGCCACGTCCAACTTAACCT‑CCGTACGCGCGCAAAGCTCC‑GCCGACAC‑GAGCCGAAGCCCCCCACCCGCCCCGAGTTTGC‑CGTCCCG‑AACGCCC‑Tggccccccctccc                                                                                                                                                     >  2:215839/1‑227 (MQ=255)
                                                                                    ggCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCCGGAAGTTCAAACTTAACCC‑CCGGACGCGCGCAATGTTCC‑CCGAACAA‑GAACCCATGGCCCCCACCCACGCGGAGTTTGC‑GGTTCTC‑AAGACAC‑TTGTCATGCACCcgc                                                                                                                                                    >  2:476903/1‑238 (MQ=255)
                                                                                         gaccatcTACATACCAAAACCGTATACCCGGCGTG‑ACTTGCTGCCCCTCTCCCGTGGTGTGCTGCGGCGTCGGGGGAACGAACGGCAAGGCGCACCTTGACAGGAGGGGGGGTTATGTT‑CTTTGGGTAAGTTCAAATTTACCCT‑CGGTACG‑GGGAAATTTTCCGGCTAAGAG‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAg                                                                                                                                               <  2:2120922/232‑1 (MQ=255)
                                                                                           acgacTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGGAAGATCAAAATTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCCCTCACCAGCGGGGAGGTTGC‑GGGAATG‑AAGGCAA‑TGGGGCGCCCTCCGCGGGGGcc                                                                                                                                             >  1:719785/1‑238 (MQ=255)
                                                                                            cgacTAAATTCCAAAACCGTGAAACCCGCGTG‑ACCTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTAAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCg                                                                                                                                            <  1:303309/238‑1 (MQ=255)
                                                                                             gacTTCATTCACAAACCGTTTAACCCGGGTGTATCTGTTGGACCGTTCCGGGGCGGGGCGGGGTGGGCAGGGGAACGATGTGCCGGGCGCCCACCCAAGGAAAGAGGCTGTATTTG‑TTTGGGGTAAGTCAAAGCTGACCCT‑CGGTCCGGGGGAAATGTGCC‑GCGAAGCC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCg                                                                                                                                            <  2:2144379/238‑1 (MQ=255)
                                                                                             gacTACCTTCAAAACCCGTTTCCCCCGCGTG‑AACTGCGGGCCCGTACCCGTGGGGTGCTGCGGCGTCGGGCGAAGGAACTGCCAGGCGCACCGTAACAGGAGGGGGGGTTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTCCC‑GGGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAg                                                                                                                                                            <  2:1818878/221‑1 (MQ=255)
                                                                                                tACGTTCCAAAACCGTTAACCCCGCGTG‑AAGTGCTGGCCCGTGTCCGTGCGGTGGTGCGTCGTCAGGCGAACGACCTGCCAGGCGCACCGTCACAGGAAGAGGCGGTATTTG‑CTTTGGTTAAGTTCAACTTTACCCT‑GGGTCCGCGGGAAATGTCCC‑GGAAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCtctc                                                                                                                                       <  2:629494/239‑1 (MQ=255)
                                                                                                tACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCtctc                                                                                                                                       <  1:206709/239‑1 (MQ=255)
                                                                                                tACATCCCAAAACCTTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCTTCAGGCGAACGAACTGCCAGGCGCCCCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAATTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCg                                                                                                                                                                                                        <  1:731408/177‑1 (MQ=255)
                                                                                                     tCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAAATG‑CTTTTGGGAAGGTCAAACTTAACCT‑CCGTACGCGCGAAATGTTCC‑GCGAAAAC‑GCGCCGATGGCGCCCCCCCGCGGGGTGGTTTC‑GGGACTT‑AAAGCACAGGGTCACCCA‑CCGCCGGAGCGCCCCTCCcgcg                                                                                                                                  >  2:919551/1‑239 (MQ=255)
                                                                                                       cAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGGACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGGGTTTGC‑GGGACTG‑AAGGGAC‑TGGTCAGCCATCcgcgg                                                                                                                                                  >  1:1818878/1‑220 (MQ=255)
                                                                                                          aacgttttAACCCGCGTA‑AACGGCTGCCCCGTTTCCGGGCGGTGCTGCGTCGTCGGGCGAACGAACTGCCAGGCGCCCGGTCACAGGAAGAGGGGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTCCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGATCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAg                                                                                                                             <  2:551354/234‑1 (MQ=255)
                                                                                                               tttAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTTGGGAAGTTCAAAATTAACCC‑CGGTACGCGCGAAATGATCC‑GCGAAACC‑GAGCCGATGCCGCCCAACAGCGGCGGGTTTGC‑GGTACTG‑AAGGAAC‑TGGTCAGGCCTCCCCGTTAACCGCCTGCCCGCGCCAAGCcgaa                                                                                                                        >  2:675655/1‑238 (MQ=255)
                                                                                                               tttAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGGGAGCCGCTCTCCCGCGGTAAGCtgat                                                                                                                        >  1:158610/1‑239 (MQ=255)
                                                                                                               tttAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGGAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGACGCTCACCAGCGGGGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGGCAGCCATCCGCGTGAGCCGCTCTCCCGCGATACGCtgat                                                                                                                        >  1:1924590/1‑239 (MQ=255)
                                                                                                                    cccGCGTG‑ACCTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTCCGGTAAGTTCAAACTTACCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAAcc                                                                                                                   <  1:1271240/239‑1 (MQ=255)
                                                                                                                       gcgTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CCTTCGGGAAGTTCAAAATTAACCT‑CGGGACGCGCGGAATGTTCC‑CCGAAGAC‑GAACCGATGCCGCCCCCCACCGGGGGGTTTGC‑GGGACTG‑AAGGCAC‑TGGGCAGCCACCCGCGGGAGCCGCGCTCCCCCGCTATGCTGCTGaaaccg                                                                                                                 >  2:34539/1‑234 (MQ=255)
                                                                                                                       gcgTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAAATG‑CTTTCGGTAAGTTCAAACCTAACCT‑CGGTACGCGCGAAAAGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGCGa                                                                                                                                                                                          >  1:1205474/1‑168 (MQ=255)
                                                                                                                         gTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGGAATTG‑CTTTCGGGAAGTTCCAAATTAACCC‑CGGGACGCGCGGAATGTTCC‑CCCAAGAC‑GAACCGCTGCCCCTCCCCCCCCGGGAGGTTGT‑GGTACTG‑AAAGCAC‑GGGGCCGCCCTCCCCCGGAGCCCCTCTCCCCCGATCAGCTGGTGGAcccttc                                                                                                               >  2:1990458/1‑234 (MQ=255)
                                                                                                                            acacggcggtcccGTATCCGTGCGTGGCTGCGTCTACGGGTCACCTATCTGCCAGGCGCACCTGCACGGGAGGTGGCGATAATTG‑CTGTCGGTCACTTAAAACTAAGCCT‑GGCTCGGTCGGAAATGTGCC‑GCGAGGGC‑GAGCCGATGCCGCTCACCAGCGCTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCACg                                                                                                            <  2:1232633/229‑1 (MQ=255)
                                                                                                                                ggctgGCCCGTACCGGCGGGCGGCTGGGCCGTAAGGGGAACAACCTCCCAGGCGACCCGTCACAGGAGGAGGCGGTATTTG‑CTTTCGGTAAGTTCAACCTTACCCT‑CGGTACGCGCGAAGTGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGt                                                                                                        <  2:1670237/238‑1 (MQ=255)
                                                                                                                                  ctgGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑CTTTCCGTAAGTTCAAACCTAACCT‑CGGTACGCGCGAAAAGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGGGGTTGC‑GGTACTG‑GAGGCAC‑CGGTCAGCCATCCGCGTGCGCCGCCCTCCCGCGATAAGCCGCAGAACCCTGcccccgggc                                                                                                       >  2:2112892/1‑232 (MQ=255)
                                                                                                                                  ctgGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAAATG‑CTTTCGGTAAGGTCAAACCTAACCT‑CGGGACGCGCGAAAAGTTCC‑GCCGAGAC‑GAGCCGATGCCGCCCCCCCACGGGGAGTTTGC‑GGGACTG‑AAGGGAC‑TGGTCAGGCATCCCCGTGGGCCCCGCTCCCCCCCTCAGCTGATGGACCtttgccccggtcc                                                                                                      >  2:595044/1‑228 (MQ=255)
                                                                                                                                          tATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGGAATTG‑CTTTCGGTGAGGTCAAACTTAACCC‑TGGAACGCGCGGAATGGTCC‑GCGAAAAG‑GGGCCGATCCCCCCCCCCCGCGGGGGGTTTGC‑GGGTCTGAAAGGCAC‑‑GGTCCGCCACCCCCGGGGCGCGCCCGCCCGCGATAAGATGATGAACCTGGCCCGTGGGCGTGAagat                                                                                               >  2:1880059/1‑238 (MQ=255)
                                                                                                                                                tGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCATCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑TCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGAGTTGTC‑GGGACTT‑AAGGCAC‑TCGTCCGCCATCCGCGTGAGCCCCGCTCCCGCGATAAGCTCATGAAACTTCCCCGCGGTGGTGAATATTCCCCa                                                                                         >  2:1830719/1‑238 (MQ=255)
                                                                                                                                                tGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTT‑GCTTCGGTAAGGTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGCAGAC‑GAGCCGATCCCGCCCCCCCGCGGGGTGTTTGC‑GGGACCT‑AAGGAAC‑TGGTCCGCCCACCCCGCGAGCCCCCCTCCCCCGCGAAGCCGATGACCCTTTCCCGCGGTCGTGAAGATTCCGcca                                                                                        >  2:40594/1‑237 (MQ=255)
                                                                                                                                                ggCGGTGCTGGGTCGTCAGGCGAACGAACTGCCAGGCGCCCCATCAAGGGAGGAGCGGGTAGTTG‑CTTTCGGTAAGTACAAACTTACCCT‑CGGCACGGGCAAATTGGCCC‑GGGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCa                                                                                         <  2:894440/237‑1 (MQ=255)
                                                                                                                                                     tgctgcGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGaa                                                                                   >  1:680018/1‑239 (MQ=255)
                                                                                                                                                     tgctgcGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGGAATTG‑CTTTCTGGGAGATCAAAATTAACCT‑CGGTACGCGCGAAATGTTTC‑GCGCAGAC‑GAGCCGATCCCGCCCACCAGCGGTGAGATTGC‑GGGACTG‑AAGGCGA‑CGGGCCGACATACCCGCGGGGCGCCCTCCCCCGACAAGGTTATTAAACCTGTCCGCGGGTGTGAAGATTcccccactgaa                                                                                   >  2:130919/1‑230 (MQ=255)
                                                                                                                                                     tgatgcGTCGTCAGGGGAACGAACTCCCGGGCGCACCGTCACAGGAGGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGGGAAATTTTCC‑GCGAAGCC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGct                                                                                                                                          <  1:2016116/181‑1 (MQ=255)
                                                                                                                                                      gctgcGTCGTGAGGAGAACAACATGCCAGGCACACGGTCACAGGAAGAGGCGGTAATTG‑CTTTGGGTAAGTTCAAACTTACCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGTaa                                                                                   <  1:841082/238‑1 (MQ=255)
                                                                                                                                                      gctgcGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTATTTG‑CTTTCGGTAATTTCAAACTGACCCT‑CGGTACGGGCGAAATGTTCC‑GCGAAGCC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGaa                                                                                   <  1:16645/238‑1 (MQ=255)
                                                                                                                                                      gcgtgtgcgtcAGGCAAAGTACCTGCCAGGCGCCCCGTCACAGGAGGGGGCGTTAATTG‑CTTTCGGAAAGTTCAACCTTACCCT‑CGTGACCCGCGAAATTTTCC‑GCAAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGaa                                                                                   <  2:1020575/231‑1 (MQ=255)
                                                                                                                                                               tcAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCC‑CGGGACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCCCCCCCGCGGTGGGTTTGC‑GGGACTG‑ACGGCCC‑TGGTCAGCCATCCGCGGGAGCCGCCCTCCCGCGATAAGCTGATGAACCTTTCCCGGGGTCGGGAATATTCCGCCAGTGAACGCCCCCTCg                                                                         >  2:1373610/1‑239 (MQ=255)
                                                                                                                                                                   gcgcACGACCGGCCAGGCGCACCGTCGCGGGAAGGGGAGGTAATTG‑TTTCCGGTAAGTTCAAACTTACCCT‑GGGTACGGGAGAAGTGTTGC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACg                                                                      <  2:668949/238‑1 (MQ=255)
                                                                                                                                                                     gaacgaacTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTAAAACTTACCCG‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTg                                                                    <  1:2021467/238‑1 (MQ=255)
                                                                                                                                                                      aacgaacTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGCTAAGCTGATGAACCTTGCCCGGGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGcc                                                                  >  1:188253/1‑238 (MQ=255)
                                                                                                                                                                           acTGCCAGGCGCCCCGCCACAGGAAGGGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTACCCT‑CGGTGCGCGGGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGAttt                                                             <  1:519035/239‑1 (MQ=255)
                                                                                                                                                                             tGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGGAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTTC‑GGTACTG‑AAGGCAC‑GGGTCAGCCATCCGCGTGAGCCGCCCTCCCGCGATAAGCTGATGAACCTTTTCCCGGGGCGGGAAAATTCCGCAATGGAACGCCCCCTCGCCGTGCCGACTTc                                                            >  2:1808084/1‑238 (MQ=255)
                                                                                                                                                                               ccAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGAGTTTGC‑GGGACTG‑AAGGCAC‑TGGTCAGCCCTCCGCGTGAGCCGCCCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCCATGGAACGCTCCATCGACGTGCAGGTTTcgcg                                                         >  1:1750559/1‑239 (MQ=255)
                                                                                                                                                                                cAGGCGCACCGTCACAGGAAGAGGCGTTAATTG‑CTTTCGGTAAGTTCAAACTTACCCG‑CGGTCCGCGGGAAATGTTCC‑GGGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGt                                                        <  1:1607465/239‑1 (MQ=255)
                                                                                                                                                                                     cccccgcccagggAAGAGGCGGTATTTC‑TTTGCGTTAAGTTCAAACTTACCCT‑CGGTGCGCGCGAAAGGTTCC‑GCGAAGAG‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTgcg                                                   <  2:1750559/228‑1 (MQ=255)
                                                                                                                                                                                         cttCACAGGAAGGGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTACCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATa                                                                                                                               <  1:16469/157‑1 (MQ=255)
                                                                                                                                                                                          tgaacaGGAGGGGGGGTTAATTT‑CTTTGGTTAAGTACAACTTTAACCT‑CGGTGCGCGGTAAGTTTCCG‑GGGAGGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCa                                              <  2:1543541/236‑1 (MQ=255)
                                                                                                                                                                                          gTCGCAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTCCAAACTTACCCG‑CGGTACGCGCGAATTGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCa                                              <  1:1119816/239‑1 (MQ=255)
                                                                                                                                                                                           tCACGGGAAGAGGGGGTAATTT‑TTTGCGGTAAGTCCAACCTTAACCT‑CGGTCCGCGGAAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCa                                              <  1:328522/238‑1 (MQ=255)
                                                                                                                                                                                                 gAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGGGga                                       >  1:294758/1‑239 (MQ=255)
                                                                                                                                                                                                  aaGAGGCGTTATTTG‑CTTTCGGTAAGTTCAAACTTACCCT‑CGGTACGCGCGAATTGTTCC‑GCGAGGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCAtggtg                                         <  1:844673/236‑1 (MQ=255)
                                                                                                                                                                                                  aaGAGGCGGTAATTG‑TTTGCGGCAAGGTCACACTAACCCT‑CGGTACGCGGGAAGTGTTCC‑GCAAAGCC‑GCGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGga                                       <  1:919551/238‑1 (MQ=255)
                                                                                                                                                                                                       gCGGTAATTG‑CTTTCGGTCAGTTCAAACTTAACCC‑CGGGACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCCCACCAGCGGGGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGGCAGCCATCCGCGTGAGCCGCTCTCCCCCGCTAAGCGGATGAAACTTTCCCGCGGTCGTGAATATTCCCCAATGGAACGCCCCATCGACGTGCAGGTGTTGCGTCTGCCCCGCCTGGTGGGagaaga                                 >  2:1206825/1‑239 (MQ=255)
                                                                                                                                                                                                       gCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGGGAGTTTGC‑GGGACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGCTAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGGagaaga                                 >  1:207397/1‑239 (MQ=255)
                                                                                                                                                                                                       gCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCC‑CGGTACGCGCGAAATGTTCC‑GCGGAGAC‑GAGCCGATGCCGCCCACCAGCGGTGAGTTTGC‑GGTACTG‑AAAGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCCCCCGCGGTAAACTGATGAACCTTGGCCGTGGTCGGGAATATTCCGCCACGGAACCCTCCATCGGCGGGCAGATTTCACGTCTTCGCCGCACGGTGGGaggaga                                 >  2:1044507/1‑239 (MQ=255)
                                                                                                                                                                                                                   tttCGGTAAGTTCAAACTTACCCG‑GGGTCCGCGCGAAATTTTCC‑GCGAAGAG‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCAt                       <  1:1701725/238‑1 (MQ=255)
                                                                                                                                                                                                                   tttCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGGGGTCGTGAATATTCCGCAATGGAACGCCCCATCGACGTGCAGATTTCCCGTCTGCGCCCCATGGTTGAAGAAGATCCCGCGCaa                       >  2:1194353/1‑237 (MQ=255)
                                                                                                                                                                                                                    ttCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGgaagaa                                   >  1:2109969/1‑225 (MQ=255)
                                                                                                                                                                                                                    ttCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATcc                     >  1:870631/1‑239 (MQ=255)
                                                                                                                                                                                                                         tAAGTTCAAACTTACCCC‑CGTCCCGCGCGAAATGTCCC‑GCGAGGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt                 <  1:418866/238‑1 (MQ=255)
                                                                                                                                                                                                                         tAAGTTAAAACTTACCCC‑CGGGACGCGGGAATTTTCCC‑GCG‑AGACGGAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt                 <  2:602260/238‑1 (MQ=255)
                                                                                                                                                                                                                         aaagtcaaatcTTACCCT‑CGGTACGCGCGAAATGTTCC‑GCAAGGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt                 <  1:722086/228‑1 (MQ=255)
                                                                                                                                                                                                                          aagggtaaaCCTAACCCCCGGTAGGGGGGAAATTTTCG‑GCGAAGCG‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGt                 <  1:2198091/232‑1 (MQ=255)
                                                                                                                                                                                                                            gTTCAACCTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGc                                                                                                                                                                      <  1:1001476/87‑1 (MQ=255)
                                                                                                                                                                                                                            gTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGc                                                                                                                                                                      >  2:1001476/1‑87 (MQ=255)
                                                                                                                                                                                                                                    ttAACCT‑CGGTCCGCGCGAAATGTCCC‑CGGAAGAG‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGa       <  2:1924590/237‑1 (MQ=255)
                                                                                                                                                                                                                                       cccT‑CGGTCGGCGCAAAATGTCCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCgtc  <  1:1990458/238‑1 (MQ=255)
                                                                                                                                                                                                                                       aCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCg    >  2:627886/1‑237 (MQ=255)
                                                                                                                                                                                                                                       aCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCg    >  1:341098/1‑237 (MQ=255)
                                                                                                                                                                                                                                       aCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCg    >  1:1373263/1‑237 (MQ=255)
                                                                                                                                                                                                                                       aCCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGCGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCAGCCGCGTTACATTCAGACCg    >  2:1717530/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
CTTCGTAGTCAGAGCAACCCGATGCCGATCATTATGGTGACGGCGAAAGGGGAAGAAGTGGACCGTATCGTAGGCCTGGAGATTGGCGCTGACGACTACATTCCAAAACCGTTTAACCCGCGTG‑AACTGCTGGCCCGTATCCGTGCGGTGCTGCGTCGTCAGGCGAACGAACTGCCAGGCGCACCGTCACAGGAAGAGGCGGTAATTG‑CTTTCGGTAAGTTCAAACTTAACCT‑CGGTACGCGCGAAATGTTCC‑GCGAAGAC‑GAGCCGATGCCGCTCACCAGCGGTGAGTTTGC‑GGTACTG‑AAGGCAC‑TGGTCAGCCATCCGCGTGAGCCGCTCTCCCGCGATAAGCTGATGAACCTTGCCCGTGGTCGTGAATATTCCGCAATGGAACGCTCCATCGACGTGCAGATTTCGCGTCTGCGCCGCATGGTGGAAGAAGATCCAGCGCATCCGCGTTACATTCAGACCGTC  >  NC_007779/4104039‑4104506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑