BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_007779	4,278,399	A→T	7.7%	I78K (ATA→AAA)	Y75_RS21180 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,278,399	0	A	T	7.7%	436.2 / 6.8	174	I78K (ATA→AAA)	Y75_RS21180	membrane protein
Reads supporting (aligned to +/- strand): ref base A (68/91); new base T (10/5); total (78/96)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 177 total aligned reads displayed.

GCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAAC  >  NC_007779/4278166‑4278636

gcgcgcAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTGATTCAT‑T‑CTCTCTatcatc                                                                                                                                                                                                                                                 >  2:1427668/1‑239 (MQ=255)

  gggcggTCCCGCCCGCATCAGTCCGCTCCGGCAGGGCCGTATAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTAtcatca                                                                                                                                                                                                                                                <  1:66326/233‑1 (MQ=255)

            gCCCGCATAGGTCCGCCCGGGCGGCGCCTTCTACGGACCCGCCGAGGGATTTTGATGATGATATTCCGTTCTGATTTGTCATTAAAA‑CAATGGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑tg                                                                                                                                                                                                                                    <  2:250890/239‑1 (MQ=255)

            gCCCGCAGGGGTCCGCTCCGGAGGGGCCGTTTAACGGCCCGCCGATGGACTTGGATGATGACATACCGTTCTGATTGGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGGGGAGGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑tg                                                                                                                                                                                                                                    <  2:887384/239‑1 (MQ=255)

                  tgcatccctcTCCGGCAGCCCCTCCAAGGGAGCCGCCGATGGATTTTGATGAGGACTTTCCGTTTTGATTTGTCATAAAAA‑CAATATGTTATATTGTTTTAAGGTGGATGATTAACGCATCTCCCAGCCATAAAAAATAAGCCTCCG‑TTATGGAGGCTTCTTCGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTtgtg                                                                                                                                                                                                                               <  2:1813110/229‑1 (MQ=255)

                      ttCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑a                                                                                                                                                                                                                         <  1:529986/238‑1 (MQ=255)

                              cGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGG‑TGTTGTGCCCG‑ATA‑ACGGTg                                                                                                                                                                                                                >  1:2116746/1‑239 (MQ=255)

                                    ccccGTCTACCGCGCCGCCGAGGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATAAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTc                                                                                                                                                                                                           <  2:525921/236‑1 (MQ=255)

                                                  ccgccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTTCTCC‑CC‑TCTATCATCACCGACCCGA‑TGTTGTGCCCC‑AAA‑ACGGGGTGGTCCGCCATTGTTAAGcc                                                                                                                                                                                            >  2:1722290/1‑239 (MQ=255)

                                                     ccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCCACCCGA‑TGTTGTGCCCA‑ATA‑GGGGTGATGTCCGCCATTGTGATGCCaa                                                                                                                                                                                          >  1:1339128/1‑238 (MQ=255)

                                                            acgttGATGATGACATTCCTTTCTAATTTAT‑ATTAAAAGCAATAATTTATATTGTTTTAAGGTGCATTATTAACCCATCTGCCAGCCATAAAAAATAAGCCTCCG‑TTATGGAGGCTTCTACTTATCAGTTCAAAATCATTTTTCATTGTGTCGGTGGCTTATTCACCT‑CT‑TCTATCATCTCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCGAGCAGccc                                                                                                                                                                                   <  1:365893/235‑1 (MQ=255)

                                                              tgttatgatggCATTCCGTTCTGATTTGTCATAAAAA‑CAATAGGTAATATTGTTTTAAGGTGGATTATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGGCCGCCATTGTGATGCCAAGCAACCCCAt                                                                                                                                                                                <  1:904974/235‑1 (MQ=255)

                                                                       gaCATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCCTCT‑CT‑TCTATCATCACCCACCCGG‑TGTTGTGGCAG‑ATA‑GCGGGGATTTTCGCCATTGTGGAGCCCAACAACCCCCATGCG‑AACCa                                                                                                                                                                      >  2:415998/1‑239 (MQ=255)

                                                                         tgttCGGTTTTGATTTCTCATTAAAA‑CAATAGATTATATTTTTTAAAGGTGGATGATAAAAGCTTCTCCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAAc                                                                                                                                                                    <  2:650751/237‑1 (MQ=255)

                                                                         atttCGGTACTGTTTTGCAATAAAAA‑AAATAGTTTATATTTTTTAAAGGGGGAAGTTTAAAGTCTCTCCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTACCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGAGGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCaa                                                                                                                                                                     <  2:1651588/236‑1 (MQ=255)

                                                                            tCCGTTCTGATTTGTCATTAAAA‑CCATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGGTTCATCC‑CT‑TCTATCATCACCCACCCGG‑GGTTGGGCCAG‑ATT‑GCGGGGTTGTCCGCCCTTGTGGTGCCCCGCCACCCCCTTTAG‑GACCAACA‑gg                                                                                                                                                                >  1:1651588/1‑239 (MQ=255)

                                                                            tCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGAGTTTGAATTCTCT‑CT‑TTTTTCATCACCGACCCGA‑TGTTGGGGCAG‑ATA‑GCGGTGATGTTCGCCATTGTGATGCCAAG‑AAACCCATTGCG‑AAACAACA‑GGc                                                                                                                                                               >  1:286101/1‑239 (MQ=255)

                                                                                ttCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTTATCT‑CT‑TCTATCAACACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCCACCCCCTTGCG‑AACCAACA‑GGCGGa                                                                                                                                                            >  1:1859244/1‑239 (MQ=255)

                                                                                ttCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATTT‑CT‑TCTATCATCACCGACCCTA‑TGTTGGGCCAG‑ATA‑ACGGGGATGTCCGCCATTGTGATGCCAAGCAAACCCATTGCG‑AACCAACA‑AGCGGa                                                                                                                                                            >  1:112106/1‑239 (MQ=255)

                                                                                ttCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGTTGAATTATCT‑CT‑TTTATAAATAACGGCCCCA‑TGTTTGGTCAG‑AAA‑AGGGGGATTGTGGCCATTGTGATGCCCAGCAAACCCCTTTTG‑AACCAAAA‑GGCGGa                                                                                                                                                            >  2:323847/1‑239 (MQ=255)

                                                                                    gATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTGTGATTTATCT‑CT‑TTTATCATCACCAACACGA‑TGTTGTGCCCG‑ATA‑ACGGGGATGTTCGCCATTGTGATTCCCAGCAACCCCATTGCG‑AACCAACA‑GGCGGaaaaa                                                                                                                                                        >  1:6769/1‑237 (MQ=255)

                                                                                     aTTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTGATTCATCT‑CT‑TTTATCATCACCCACTCCC‑TGTGTTGCCCG‑ATA‑GCCGTGATGTCCCCCCTTTTTATCCCACGCAAACCCCTTCCG‑AACAAACA‑GGCGGAaacaa                                                                                                                                                       >  2:711803/1‑239 (MQ=255)

                                                                                        tGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAACCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTCATTCATCC‑CT‑TTTATCAACACCGCCTCGATTGTTG‑GCCAG‑ATA‑ACAGTGATATCACTCATTTTTATGCCAAACAACACCACTGCC‑AACCAAAA‑GGCgggcaaaaac                                                                                                                                                     >  2:1839955/1‑230 (MQ=255)

                                                                                         gTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGcc                                                                                                                                                   <  1:1225209/239‑1 (MQ=255)

                                                                                                    cAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCT‑TTATGGAGGCTTCTACGTATCAGTTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCgctg                                                                                                                                        <  1:1381279/239‑1 (MQ=255)

                                                                                                       tAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGctgctg                                                                                                                                     >  1:1329886/1‑239 (MQ=255)

                                                                                                         ggTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCaa                                                                                                                                                                     <  1:408275/207‑1 (MQ=255)

                                                                                                           ttATATTGTTTTAGGGTGGATGATTAAAGCATCGGCCAGCCATAAAAAAGAAGCCTCCG‑TGATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GAc                                                                                                                                             <  1:1517927/227‑1 (MQ=255)

                                                                                                              tatTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑a                                                                                                                                                                                                                         <  1:757805/152‑1 (MQ=255)

                                                                                                                   ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTGTGATTCATCT‑CT‑TCTATCATCACCGACTCGG‑ATTTGTGGCAG‑ATA‑GCGGTGATGTCCGCCATTTTGATGCCAAGCAAACCCATTTCG‑GACCAACA‑GGCGGCAAAAACGCCCA‑GACCGCCGCTGATCAcccccac                                                                                                                          >  1:1022928/1‑233 (MQ=255)

                                                                                                                   ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATTACCGACTCGA‑TGTTGTGCCAG‑ATA‑TCGGTGATGTCCCCCACTTTGATGCCAAACCACCCCACTTCG‑AAACAAAA‑GGCGGAAAAAACGCCCC‑GACCGCTGCTGGTCACCGCAAg                                                                                                                          >  1:336959/1‑238 (MQ=255)

                                                                                                                   ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑tg                                                                                                                                                                                                                                    <  1:1995157/137‑1 (MQ=255)

                                                                                                                   ttttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTCTGTATCCTCT‑CT‑TCCTACATCAACGCCGCGA‑TTGTGTGCCA‑‑ATATAAGGTGATGTTCGCCATTGTTGTGGTCAGCAACCCCCTTGGG‑GAAAAATG‑GGCGGGAAACACGCCCA‑CACCGCCGCTGCTCACCGCCAGc                                                                                                                         >  2:1280109/1‑239 (MQ=255)

                                                                                                                   ttttAAGGGTGTTGTTTAAGGCATCTCCCCGCCA‑AAAAAAGAAGGCCCCGTTTAGGGAGGTTTTTAGGTATCAGTTAAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGc                                                                                                                         <  2:1542677/239‑1 (MQ=255)

                                                                                                                    tttAAGGTGGTTAATTAAAGCTTTTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTACCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGGCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGc                                                                                                                         <  1:711803/238‑1 (MQ=255)

                                                                                                                    tttAAGGTGGATGATAAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGGGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑TACCGCTGCTGATCACCGCAAGc                                                                                                                         <  1:475631/238‑1 (MQ=255)

                                                                                                                     ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTATTCATCT‑CT‑TCTATCATCACCCACTCGA‑TGTTGTGCCCG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAAACCCCTTGCG‑AACCAAAA‑GGCGGAAACAACGCCCA‑GACCCCTGCTGATCACCGCAAGccc                                                                                                                       >  1:1826054/1‑239 (MQ=255)

                                                                                                                     ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGTATCATCT‑CT‑TCTATCATCACCCACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAACAAACCCACTGCG‑AACCAAAA‑AGCGGCAACAACGCCCC‑CAACCCTGCCCATCACCCCAAccc                                                                                                                        >  2:1915624/1‑238 (MQ=255)

                                                                                                                     ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTTGTTCATCTCCT‑TCTTTC‑TCACCCCACCCA‑TGTTGGGCCAGAAAA‑GCGGTG‑TATTCGGCACTGTGTAGCCCACGAACCCCCTTTCG‑AACCAACA‑GGGGGAAACAACGCCCA‑AACAGCCGCCGATCACCGccaacc                                                                                                                        >  2:1406904/1‑233 (MQ=255)

                                                                                                                     ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTGATTTATCT‑CT‑TCTATCATCACCAACTCGA‑TGTTGTGCCAG‑ATA‑GCGGGGATTTCCGCCATTGTGATGCCAAGCAACCCCCTTGCG‑AACCAACA‑GGCGGAAAAAAAGCCCA‑GACCGCTGCCGATCACCCCAAAcc                                                                                                                        >  1:494066/1‑238 (MQ=255)

                                                                                                                     ttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGGCTGATTCATCT‑CT‑TTTATCATCACCCACCCGA‑TTGTGGGGCAG‑ATA‑GCGGGGATGTCCGCCATTTTGATTCCCAGACACCCCACTGCGAAACCAAAA‑GGCGGAAA‑AACGCCCC‑GGCCCGCGCTCATCTCCGCaaagc                                                                                                                        >  2:915511/1‑235 (MQ=255)

                                                                                                                       aaGGTGTATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑GTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCTTTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCTCCGCAAGCCCGa                                                                                                                     <  1:915850/239‑1 (MQ=255)

                                                                                                                       aaGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGa                                                                                                                     <  1:2024420/239‑1 (MQ=255)

                                                                                                                        aGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGTCTGATTTATTT‑CT‑TTTATCATCACCCACTCGA‑TGTTTTGCCAG‑ATA‑GCGGGGGTGTCCCCCCTTTTGGTGCCCAGCAACCCCCTTGCG‑AACCAAAAGGGCGGAAA‑AAAGCCCA‑AACCCCCGCCGAACCCCCCAAAccccc                                                                                                                     >  2:1843269/1‑236 (MQ=255)

                                                                                                                             gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTTTTCC‑CT‑TCTATCATTAACCACTCGA‑TCTTTTGCCAC‑ATT‑ACTGGGCTGTCCGCCCTTGTGATGCCCAACCACCCCGTTGCG‑AACCAACA‑GACGGCAAAAAAACCCACAACCCCGCCGGATC‑CCGCAAGCCCCACAC‑AAt                                                                                                              >  2:285393/1‑239 (MQ=255)

                                                                                                                             gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCCTTT‑CT‑TTTATTATCACCGACCCGA‑TGTTTTGCCAG‑ATA‑GCGGGGATGTCCGCCCTTGTGATGCCCAACAAACCCCATGCG‑AACCAAAA‑GGGGGGAACAAACCCCA‑CACCCCCGCCGAACACCCCCACCCCGACAT‑aa                                                                                                               >  2:1046180/1‑238 (MQ=255)

                                                                                                                             gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTTTGATGCCAAGCAAACCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑AAACGCTGCTGATCCCCGCAAACCCGACAA‑AAt                                                                                                              >  1:2047583/1‑239 (MQ=255)

                                                                                                                             gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCCACTCGA‑TGTTGTGCCAG‑ATA‑GCGGGGATGTCCGCCCTTTTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCCCAAGCCCGACAT‑aa                                                                                                               >  1:1315917/1‑238 (MQ=255)

                                                                                                                             gatgatTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCAACACCGCCTCGG‑TGTTGTGCCCG‑ATA‑GCGGTGATGTCCCCCATTGTGTTGCCAAGCAACCCCATTCCG‑AACCAACA‑GGCGGAAACAACGCCCC‑GACCGCTGCTGATCACCGCAAGCCCCACAT‑AAt                                                                                                              >  1:600588/1‑239 (MQ=255)

                                                                                                                                 atTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GAc                                                                                                                                             <  1:1477504/205‑1 (MQ=255)

                                                                                                                                 atTAAACCTTCTCCACGCCAAAAAAAAGATGCCTCCG‑TTATGGAGGCTTTTACTTTTCCGTTCAAAATCATTGGCCATTGGGGCGGTGTCGGATTCATCT‑CT‑TCTACCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGa                                                                                                          <  2:1504320/239‑1 (MQ=255)

                                                                                                                                  ttAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGa                                                                                                          <  1:1742560/238‑1 (MQ=255)

                                                                                                                                     aaGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGAATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTATTCATCT‑CT‑TCTATCATCACCGACACGG‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCCAGGAACCCCATTGCG‑AACCAACA‑GGCGGAAACCACGCCCA‑GACCGCCGCTGAATACCGCCAACCCCACAT‑AATCacaacc                                                                                                       >  1:282196/1‑233 (MQ=255)

                                                                                                                                          tCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTTCGCCATTTTGATGCCAAGCAACCCCCTTTCG‑AACCAACA‑GGCGGAAACAACGCCCC‑GACCGCTGCTGATCACCCCCAACCCGACAA‑AATCAGACCGACGaaa                                                                                                 >  1:924455/1‑239 (MQ=255)

                                                                                                                                           cTGCCAGCCATAAAAAAGAAGCCGCCG‑TTATGGAGGCTTCTCCGTATCAGGTCAAAATCATTGGCCAGTGTGGCGGTGTCGGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGaaaa                                                                                                <  1:1362729/239‑1 (MQ=255)

                                                                                                                                                agccaTAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTAGCGGTGTCTTATTCAACT‑CT‑TCTATCACCACCCACTCGA‑TTTGTTGACAC‑ATA‑ACAGCAGTGTACACCATTTAGATGCCCCGCCACCCCCTTCCG‑GACCAACC‑AGCAGGAACAACCCCAC‑AACCGCCACTGATCACCACCAGCCCgcacaaat                                                                                                               >  1:1585420/1‑213 (MQ=255)

                                                                                                                                                  ccaTAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTATGTATCAGGTCAAAATCATTGGCCATTGTGGCGGGGGTTGGTTCATTT‑CT‑TCTTTTATCACCCACCCGA‑AGTTGTGGCCG‑ATA‑GTGGTGGAGTTCGCCACTTTGGTGCCAACCAAACCCATTGCG‑CAACAAAA‑GGCGGAAACAACCCACA‑GAACGCCGCTGCTCCACGCAAGCCCGACAC‑AATCAGAACGCCGCAAACgggagg                                                                                         >  2:564891/1‑235 (MQ=255)

                                                                                                                                                  ccaTAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGCGGATGATTTATCT‑TT‑TCTTACATCACCGACAAGC‑TTTTGTGCCCG‑ATA‑ACGGTGATGTTCGCCACTGAGCAGCCAAGAAACACCAATGCG‑AACCAACA‑GGAGGAAAAAACGCCCC‑GACCGCCGCTGATCACCGCAACACCCACAT‑ACTCAAACCCACGaaaaacgaagt                                                                                         >  2:651369/1‑232 (MQ=255)

                                                                                                                                                   caTAAAAAAAAGGCCTCCG‑TTATGGAGGTTTTTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCGGATTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TTTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGAt                                                                                           <  2:720672/236‑1 (MQ=255)

                                                                                                                                                     tAAAAAAGAAGCCTCCT‑TTATGGAGCCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGa                                                                                      <  1:1792413/239‑1 (MQ=255)

                                                                                                                                                          aagaggCCTCCT‑TTAGGGAGTCTTTTACGTTTCAGGTAAAAACCATTGGCCATTGGGGCGGTGTCTGATTCATCT‑CT‑TCTTTCATCCCCGACTAGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGtgtg                                                                                 <  2:1329886/239‑1 (MQ=255)

                                                                                                                                                            gaagCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTTTCATCACCCACTCGA‑TGTTGTGCCCG‑ATA‑GCGGTGATGTCCGCCATTGTTATGCCAAACAACCCCCTTGCG‑AACCCACA‑GGCCGAAACAACACCCA‑GACCCCCGCCGATCACCCCCAACCCGACATAAATCA‑ACCGACGAAAAAGGATGTTGGgtgt                                                                                  >  2:607869/1‑236 (MQ=255)

                                                                                                                                                            gaagCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGGCTGCTTCATTT‑CT‑TCTATCATCACCGGCCCGA‑AGTTGTGCCAG‑AGA‑GCGGGGATGTCCGCCATTGTGAAGCCCAGCAACCCCATTGCG‑AACCAACA‑GGAGGAAACAACGACCA‑CACCGCTGCTCATCCCCGCAAACCCGACAT‑AATCAAACCCACGAAAAAGGAAGGTGAgtgt                                                                                  >  2:2042910/1‑236 (MQ=255)

                                                                                                                                                                 cTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGc                                                                            <  1:1046180/237‑1 (MQ=255)

                                                                                                                                                                   cgg‑TTAGGGAGCGTTTTACGTTTCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGGGCCAG‑ATA‑GCGGGGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                        <  2:1746092/237‑1 (MQ=255)

                                                                                                                                                                   ccG‑TTTGGGAGGCTTTTACGTATCAGGTCAAAATCATTTACCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCAAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                        <  2:112106/239‑1 (MQ=255)

                                                                                                                                                                   ccG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                        <  1:961593/239‑1 (MQ=255)

                                                                                                                                                                   ccG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                        <  1:415998/239‑1 (MQ=255)

                                                                                                                                                                    cgttttgggAGGTTTTTACGGATCAGGTCAAAATCATTGGCCATTGTGGGGGTGTCTGTTGCATCT‑TT‑TAAATCATCACGGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGa                                                                        <  2:772347/232‑1 (MQ=255)

                                                                                                                                                                         ggggatgtttCTACGTTTCGGGTAAAAATCTTTGGCCATGGGGGGGGTGTCTGATTCACCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATG‑GCGGTGATGCCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAAcat                                                                   <  2:1380234/231‑1 (MQ=255)

                                                                                                                                                                               gtttttACGTTTCAGGTCAAAATCATTGGCCTTTGTGGGGGTGTCTGATTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                              <  2:618916/233‑1 (MQ=255)

                                                                                                                                                                               gCTCCTCCGTATCAGGTCAAAATCTTTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                              <  1:650803/238‑1 (MQ=255)

                                                                                                                                                                                cTTCTACGTATCGGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACGCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                              <  1:1918387/237‑1 (MQ=255)

                                                                                                                                                                                 ttCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACg                                                            <  1:524274/238‑1 (MQ=255)

                                                                                                                                                                                    tACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTTATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑ACGGTGATTTCCGCCATTGTGATGCCAAACAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGc                                                          >  1:618916/1‑237 (MQ=255)

                                                                                                                                                                                    tACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTa                                                              <  1:1937741/233‑1 (MQ=255)

                                                                                                                                                                                     tcGTATCAGGTCAAAATCATTGGCCATGTTGGCGGTGTCTGAGTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACt                                                       <  1:836641/238‑1 (MQ=255)

                                                                                                                                                                                         aTCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTAATTT‑CT‑TCTATCATCACCGACCCGC‑TGTTGTGCCAG‑AAC‑GCGGGGATGTCCGCCATTGTGATGCCAAGAAACCCCCCTGCG‑AAACAAAA‑GGCGGAAACAAAGCCCA‑GACCGCTGCTCGTCACCCCCCGCCCGACAG‑AAACAAAGCGCCGAAAACGGAGGTTGAGTGGGCTGGCGCGAAACAACATTACAGCaacaaaa                                                   >  2:1647787/1‑233 (MQ=255)

                                                                                                                                                                                             ggTCAAAATCATTGGCCATTGTGGCGGTGTTTGATTTATCT‑CT‑TCTATCCTCCACCACTCGA‑TTTTGTGCCAC‑ATA‑GCGGGGATGTCCGCCCTTGTTATGCCCAACAACCCCCATGCG‑AAACCACA‑GGCGGGAACAACGCCCA‑GACCGCCCCTGATCCACGCAAGCCCGACAT‑AATACAACCGACAAAAACGGAAGGTTAGCGGGCTGGCCAGCAACAAACTTACGGCCCtcaaaaagt                                               >  2:823770/1‑231 (MQ=255)

                                                                                                                                                                                               acAAAATCATTGGCCTTGGGGGAGGTGTTGGATTCATCT‑CT‑TCTTTAATCACCGACTCGA‑TTGTGTGCCAG‑ATA‑GCGGTTAGGTCCGCCATTGGGAGGCCAAGCAGCCCCATAGCG‑AACCAACA‑GGCGTAAACAACGCCCA‑GCCCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGGCGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGc                                             <  2:336959/238‑1 (MQ=255)

                                                                                                                                                                                                  aaaTCATTGGCCTTTGTGGCGGTGTCTTATTCATCC‑CT‑TCTATCACCGCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGAGGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                                          <  1:514032/239‑1 (MQ=255)

                                                                                                                                                                                                  aaaTCATTGGCCATTGTGTCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                                          <  1:1626618/239‑1 (MQ=255)

                                                                                                                                                                                                  aaaTCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAg                                          <  1:323847/239‑1 (MQ=255)

                                                                                                                                                                                                       aTTGGCCATTGTGGCGGTGGCTGATTCATCT‑CT‑TCTATCATCACCGCCTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaag                                     <  1:1582785/239‑1 (MQ=255)

                                                                                                                                                                                                        ttGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCgaaga                                    <  1:577156/239‑1 (MQ=255)

                                                                                                                                                                                                             cATGGTGGCGGTGTCTGATCCACTT‑CT‑TCTACCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAAc                               <  1:1974474/239‑1 (MQ=255)

                                                                                                                                                                                                               ttGGGGCGGTGTCTGATTCATCT‑CT‑TCTATCACCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCa                             <  1:804573/239‑1 (MQ=255)

                                                                                                                                                                                                                tgtgGCGGTGTCTGATTCATCT‑CT‑TCTATCTTCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGc                                                                                                                         <  1:1239457/147‑1 (MQ=255)

                                                                                                                                                                                                                   gggggTGTCTGATTCATCT‑CT‑TCTATCATCGCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCAATTGGGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGc                          <  1:1265766/235‑1 (MQ=255)

                                                                                                                                                                                                                   ggCGGTGTCTGATTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGc                          <  1:252548/238‑1 (MQ=255)

                                                                                                                                                                                                                   ggCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGc                          <  1:1406904/238‑1 (MQ=255)

                                                                                                                                                                                                                     cGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCa                        <  1:607869/238‑1 (MQ=255)

                                                                                                                                                                                                                        tgtCTGATTCATCT‑CT‑TCTATCATCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAgtg                     <  1:1843269/238‑1 (MQ=255)

                                                                                                                                                                                                                               ttCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCAAG‑ATA‑GCGGTGAGGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGt             <  1:1704651/239‑1 (MQ=255)

                                                                                                                                                                                                                                    ct‑ct‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGAGAGCCa        >  1:925330/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ct‑ct‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGCGCCGGCCAGAAACATCATTACGGCAATAAGACGTGGCCAGAGAACAAGAACCACGCCAGTGGTGCGCGTAGCCa        >  2:1310569/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ct‑ct‑TCTATCACCCCCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCa        <  1:760991/239‑1 (MQ=255)

                                                                                                                                                                                                                                          tctATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAAAGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCaa    >  2:233184/1‑239 (MQ=255)

                                                                                                                                                                                                                                           ctATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCaa    >  1:2034439/1‑238 (MQ=255)

                                                                                                                                                                                                                                            tATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAAc  >  1:290948/1‑239 (MQ=255)

GCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAA‑CAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCG‑TTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCT‑CT‑TCTATCATCACCGACTCGA‑TGTTGTGCCAG‑ATA‑GCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCG‑AACCAACA‑GGCGGAAACAACGCCCA‑GACCGCTGCTGATCACCGCAAGCCCGACAT‑AATCAGACCGACGAAAACGGATGTTGAGTGTGCTGGCCAGAAACATCATTACGGCACTAAGAAGTTGCCAGCGAAGAAGAACCACGCCAGTGGTGAGGGTAGCCATCAAAC  >  NC_007779/4278166‑4278636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`